Nerve growth factor-mediated paracrine regulation of hepatic stellate cells by multipotent mesenchymal stromal cells

Lin, Nan; Hu, Kunpeng; Chen, Si; Xie, Shi-Bin; Tang, Zhaofeng; Lin, Jizong; Xu, Rui‐hua

doi:10.1016/j.lfs.2009.06.007

Cited by 52 publications

(41 citation statements)

References 25 publications

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“…There is evidence that BMSCs can enhance apoptosis of activated HSCs through secreting paracrine factors (Parekkadan et al, 2007). These results are consistent with our previous studies (Lin et al, 2009). Using the Transwell coculture model, we have also found that HSCs indirectly modulate the proliferation and differentiation of BMSCs in vitro via the hedgehog-mediated paracrine signaling pathway (Lin et al, 2008).…”

Section: Introductionsupporting

confidence: 92%

“…Using a Transwell coculture assay, our previous study suggested that BMSCs promote apoptosis of HSCs. This effect was mediated in part through paracrine signaling by NGF secreted by BMSCs (Lin et al, 2009). The present data indicate that BMSCs in direct cell-cell contact not only result in growth inhibition in HSCs compared with the Transwell system, but also suppress HSC activation in vitro (Fig.…”

Section: Discussionsupporting

confidence: 51%

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Activation of Notch1 signaling by marrow-derived mesenchymal stem cells through cell–cell contact inhibits proliferation of hepatic stellate cells

Chen

Lin

et al. 2011

Life Sciences

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Section: Introductionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 51%

Activation of Notch1 signaling by marrow-derived mesenchymal stem cells through cell–cell contact inhibits proliferation of hepatic stellate cells

Chen

Lin

et al. 2011

Life Sciences

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“…However, the underlying mechanisms are not yet well understood. Therefore, in vitro models and further experimentation are required to better understand how MSCs modulate HSC activation and their paracrine properties [23–25]. Ideally, these systems should use human-derived cells since rodent cells may behave and react differently.…”

Section: Introductionmentioning

confidence: 99%

Human liver mesenchymal stem/progenitor cells inhibit hepatic stellate cell activation: in vitro and in vivo evaluation

et al. 2017

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BackgroundProgressive liver fibrosis leads to cirrhosis and end-stage liver disease. This disease is a consequence of strong interactions between matrix-producing hepatic stellate cells (HSCs) and resident and infiltrating immune cell populations. Accumulated experimental evidence supports the involvement of adult-derived human liver mesenchymal stem/progenitor cells (ADHLSCs) in liver regeneration. The aim of the present study was to evaluate the influence of ADHLSCs on HSCs, both in vitro and in vivo.MethodsActivated human HSCs were co-cultured with ADHLSCs or ADHLSC-conditioned culture medium. The characteristics of the activated human HSCs were assessed by microscopy and biochemical assays, whereas proliferation was analyzed using flow cytometry and immunocytochemistry. The secretion profile of activated HSCs was evaluated by ELISA and Luminex. ADHLSCs were transplanted into a juvenile rat model of fibrosis established after co-administration of phenobarbital and CCl4.ResultsWhen co-cultured with ADHLSCs or conditioned medium, the proliferation of HSCs was inhibited, beginning at 24 h and for up to 7 days. The HSCs were blocked in G0/G1 phase, and showed decreased Ki-67 positivity. Pro-collagen I production was reduced, while secretion of HGF, IL-6, MMP1, and MMP2 was enhanced. Neutralization of HGF partially blocked the inhibitory effect of ADHLSCs on the proliferation and secretion profile of HSCs. Repeated intrahepatic transplantation of cryopreserved/thawed ADHLSCs without immunosuppression inhibited the expression of markers of liver fibrosis in 6 out of 11 rats, as compared to their expression in the vehicle-transplanted group.ConclusionsThese data provide evidence for a direct inhibitory effect of ADHLSCs on activated HSCs, which supports their development for the treatment of liver fibrosis.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-017-0575-5) contains supplementary material, which is available to authorized users.

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“…UCMSCs are excellent seed cells for tissue engineering. Related studies have found that mesenchymal stem cells can induce the apoptosis of hepatic stellate cells through paracrine function (Lin et al, 2009). In the current study, it was found that, after non-contact co-culture of UCMSCs and endometriotic cells, the apoptosis of endometriotic cells was obviously increased compared with the control group.…”

Section: Discussionmentioning

confidence: 99%

Effect of human umbilical cord mesenchymal stem cells on endometriotic cell proliferation and apoptosis

Ln¹,

Lin²,

Bn³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The objective of this study was to observe the effects of human umbilical cord mesenchymal stem cells (UCMSCs) on the proliferation and apoptosis of endometriotic cells. Endometriotic cells and UCMSCs were primarily cultured in vitro. In the experimental group, a UCMSC and endometriotic cell non-contact co-culture system was established. The control group consisted of 1 x 10 5 endometriotic cells cultured alone. The proliferation and apoptosis of endometriotic cells were respectively detected using the MTT method and flow cytometry. The mRNA expression level of the tensin homologue gene (PTEN) in endometriotic cells was detected by reverse transcriptionpolymerase chain reaction amplification. Compared with the control group, the proliferation of endometriotic cells in the experimental group was clearly inhibited (P < 0.05) and time-dependent (P < 0.05). In addition, the number of apoptotic cells were significantly increased (P < 0.05), and the amount of cells, which entered S phase from G1 phase, decreased significantly. Furthermore, the mRNA expression level of the PTEN gene in the experimental group was significantly higher than in the control group (P < 0.05). These results suggest that UCMSCs might inhibit the proliferation of human endometriotic cells in vitro and promote their apoptosis by upregulating the expression of PTEN.

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Nerve growth factor-mediated paracrine regulation of hepatic stellate cells by multipotent mesenchymal stromal cells

Cited by 52 publications

References 25 publications

Activation of Notch1 signaling by marrow-derived mesenchymal stem cells through cell–cell contact inhibits proliferation of hepatic stellate cells

Activation of Notch1 signaling by marrow-derived mesenchymal stem cells through cell–cell contact inhibits proliferation of hepatic stellate cells

Human liver mesenchymal stem/progenitor cells inhibit hepatic stellate cell activation: in vitro and in vivo evaluation

Effect of human umbilical cord mesenchymal stem cells on endometriotic cell proliferation and apoptosis

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