Nerve growth factor (NGF) and lenses: effects of NGF in an in vitro rat model of cataract

Ghinelli, Emiliano; Aloe, Luigi; Cortês, M.M.M.; Micera, Alessandra; Lambìase, Alessandro; Bombardieri, Stefano

doi:10.1007/s00417-003-0733-6

Cited by 11 publications

(6 citation statements)

References 39 publications

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“…Although there are many reports on neurotrophins, these data are derived mostly from in vitro studies (Danzer et al 2002; Martin et al 2002; Seifer et al 2002; Takano et al 2002; Ghinelli et al 2003; Price et al 2005). From rodents to primates and in humans, in situ hybridization has provided useful information regarding the endogenous expression of mRNAs (Shelton and Reichardt 1986; Huntley et al 1992; Hayashi et al 1993,1997; Hashimoto et al 1999) and tyrosine kinase receptors (Schatteman et al 1988; Mufson et al 1991; Zhou and Rush 1993; Sandell et al 1998; Hashimoto et al 1999; Zhou and Yang 1999; Yamuy et al 2000) for members of the neurotrophin family.…”

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confidence: 99%

Immunohistochemical Distribution of NGF, BDNF, NT-3, and NT-4 in Adult Rhesus Monkey Brains

Zhang

Zou

et al. 2007

J Histochem Cytochem.

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Immunohistochemical distribution and cellular localization of neurotrophins was investigated in adult monkey brains using antisera against nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). Western blot analysis showed that each antibody specifically recognized appropriate bands of approximately 14.7 kDa, 14.2 kDa, 13.6 kDa, and 14.5 kDa, for NGF, BDNF, NT-3, and NT-4, respectively. These positions coincided with the molecular masses of the neurotrophins studied. Furthermore, sections exposed to primary antiserum preadsorbed with full-length NGF, BDNF, NT-3, and NT-4 exhibited no detectable immunoreactivity, demonstrating specificities of the antibodies against the tissues prepared from rhesus monkeys. The study provided a systematic report on the distribution of NGF, BDNF, NT-3, and NT-4 in the monkey brain. Varying intensity of immunostaining was observed in the somata and processes of a wide variety of neurons and glial cells in the cerebrum, cerebellum, hippocampus, and other regions of the brain. Neurons in some regions such as the cerebral cortex and the hippocampus, which stained for neurotrophins, also expressed neurotrophic factor mRNA. In some other brain regions, there was discrepancy of protein distribution and mRNA expression reported previously, indicating a retrograde or anterograde action mode of neurotrophins. Results of this study provide a morphological basis for the elucidation of the roles of NGF, BDNF, NT-3, and NT-4 in adult primate brains.

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confidence: 99%

Immunohistochemical Distribution of NGF, BDNF, NT-3, and NT-4 in Adult Rhesus Monkey Brains

Zhang

Zou

et al. 2007

J Histochem Cytochem.

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“…In vitro studies on cultured LECs might elucidate these aspects using pathological LECs and neutralizing factors (mediators) or control LECs and pathological AH (differentiation studies). Regarding the appearance of the EMT phenotype, the participation of other mediators such as FGF, EGF, NGF, and BDNF, which are known to be increased in AH and able to work as modulators of cell differentiation/contractile activity, cannot be excluded [ 41 , 42 , 43 , 44 , 45 , 46 , 47 ]. In previous studies, these growth factors have been reported to be profibrogenic and anti-inflammatory [ 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…Regarding the appearance of the EMT phenotype, the participation of other mediators such as FGF, EGF, NGF, and BDNF, which are known to be increased in AH and able to work as modulators of cell differentiation/contractile activity, cannot be excluded [ 41 , 42 , 43 , 44 , 45 , 46 , 47 ]. In previous studies, these growth factors have been reported to be profibrogenic and anti-inflammatory [ 46 , 47 ]. In particular, NGF has been reported to trigger the EMT phenotype and TGFβ and FGF, but the presence of two specific receptors (trkA NGFR and p75 NTR ) allows NGF to mediate a selective response in EMT cells from survival to apoptosis, suggesting additional homeostatic considerations [ 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

Biomarker Signature in Aqueous Humor Mirrors Lens Epithelial Cell Activation: New Biomolecular Aspects from Cataractogenic Myopia

De Piano,

Cacciamani,

Balzamino

et al. 2023

Biomolecules

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Inflammatory, vasculogenic, and profibrogenic factors have been previously reported in vitreous (VH) and aqueous (AH) humors in myopic patients who underwent cataract surgery. In light of this, we selected some mediators for AH and anterior-capsule-bearing lens epithelial cell (AC/LEC) analysis, and AH expression was correlated with LEC activation (epithelial–mesenchymal transition and EMT differentiation) and axial length (AL) elongation. In this study, AH (97; 41M/56F) and AC/LEC samples (78; 35M/43F) were collected from 102 patients who underwent surgery, and biosamples were grouped according to AL elongation. Biomolecular analyses were carried out for AH and LECs, while microscopical analyses were restricted to whole flattened AC/LECs. The results showed increased levels of interleukin (IL)-6, IL-8, and angiopoietin-2 (ANG)-2 and decreased levels of vascular endothelium growth factor (VEGF)-A were detected in AH depending on AL elongation. LECs showed EMT differentiation as confirmed by the expression of smooth muscle actin (α-SMA) and transforming growth factor (TGF)-βR1/TGFβ isoforms. A differential expression of IL-6R/IL-6, IL-8R/IL-8, and VEGF-R1/VEGF was observed in the LECs, and this expression correlated with AL elongation. The higher VEGF-A and lower VEGF-D transcript expressions were detected in highly myopic LECs, while no significant changes were monitored for VEGF-R transcripts. In conclusion, these findings provide a strong link between the AH protein signature and the EMT phenotype. Furthermore, the low VEGF-A/ANG-2 and the high VEGF-A/VEGF-D ratios in myopic AH might suggest a specific inflammatory and profibrogenic pattern in high myopia. The highly myopic AH profile might be a potential candidate for rating anterior chamber inflammation and predicting retinal distress at the time of cataract surgery.

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“…NGF has also been quantified in human tears, indicating that the lacrimal gland basally releases NGF, suggesting a role in the maintenance of the tear film homeostasis [84]. NGF and its receptors, TrkA and p75NTR, are also expressed by the rat lacrimal gland tissue [85, 86]. The roles played by NGF in the homeostasis of the eye and in vision are, therefore, crucial and have been widely investigated both in vitro and in vivo [80, 87].…”

Section: Ngf and Ocular Disordersmentioning

confidence: 99%

Nerve Growth Factor: Early Studies and Recent Clinical Trials

Rocco

Soligo

Manni

et al. 2018

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174

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Since its discovery, nerve growth factor (NGF) has long occupied a critical role in developmental and adult neurobiology for its many important regulatory functions on the survival, growth and differentiation of nerve cells in the peripheral and central nervous system. NGF is the first discovered member of a family of neurotrophic factors, collectively indicated as neurotrophins, (which include brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin 4/5). NGF was discovered for its action on the survival and differentiation of selected populations of peripheral neurons. Since then, an enormous number of basic and human studies were undertaken to explore the role of purified NGF to prevent the death of NGF-receptive cells. These studies revealed that NGF possesses important therapeutic properties, after topical administration, on human cutaneous pressure ulcer, corneal ulcers, glaucoma, retinal maculopathy, Retinitis Pigmentosa and in pediatric optic gliomas and brain traumas. The aim of this review is to present our previous, recent and ongoing clinical studies on the therapeutic properties of NGF.

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Nerve growth factor (NGF) and lenses: effects of NGF in an in vitro rat model of cataract

Cited by 11 publications

References 39 publications

Immunohistochemical Distribution of NGF, BDNF, NT-3, and NT-4 in Adult Rhesus Monkey Brains

Immunohistochemical Distribution of NGF, BDNF, NT-3, and NT-4 in Adult Rhesus Monkey Brains

Biomarker Signature in Aqueous Humor Mirrors Lens Epithelial Cell Activation: New Biomolecular Aspects from Cataractogenic Myopia

Nerve Growth Factor: Early Studies and Recent Clinical Trials

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