Nerve growth factor (NGF) and epidermal nerve fibers in atopic dermatitis model NC/Nga mice

Horiuchi, Yasuhiro; Bae, Sang Jae; Katayama, Ichiro

doi:10.1016/j.jdermsci.2005.03.012

Cited by 28 publications

(25 citation statements)

References 9 publications

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“…Immunostaining to detect PGP9.5, a pan-neuronal marker present as a cytoplasmic protein in peripheral nerves, is currently the most consistent method to visualize ENF. 30,31 A progressive decrease in epidermal nerve fiber density over time developed in SIV-infected macaques resembling ENF decreases reported in HIV-infected individuals. A significant decline in ENF density developed 8 weeks postinoculation and was reduced further at the 12-week postinoculation time point (P Ͻ 0.001; Figure 1).…”

Section: Epidermal Nerve Fiber Density Declines With Siv Infectionmentioning

confidence: 75%

Macrophage-Mediated Dorsal Root Ganglion Damage Precedes Altered Nerve Conduction in SIV-Infected Macaques

Laast

Shim

Johanek

et al. 2011

The American Journal of Pathology

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Section: Epidermal Nerve Fiber Density Declines With Siv Infectionmentioning

confidence: 75%

Macrophage-Mediated Dorsal Root Ganglion Damage Precedes Altered Nerve Conduction in SIV-Infected Macaques

Laast

Shim

Johanek

et al. 2011

The American Journal of Pathology

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“…Treatment with K52a led to a significant improvement of psoriatic plaques [35]. TrkA inhibition in NC/nga mice, a well-characterized mouse model of AD with increased NGF in lesional skin [36], significantly inhibited the development of scratching behavior and dermatitis and reduced nerve fibers in the epidermis [37, 38]. Thus, there is first evidence for a relevance of NGF as immunomodulator also in psoriasis.…”

Section: Discussionmentioning

confidence: 99%

Clinical Relevance of Nerve Growth Factor Serum Levels in Patients with Atopic Dermatitis and Psoriasis

Schulte‐Herbrüggen

Fölster‐Holst

Elstermann

et al. 2007

Int Arch Allergy Immunol

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Background: Nerve growth factor (NGF) is known to act as a potent mediator in neuroinflammatory processes. Recent studies point to a role of NGF in the skin pathophysiology of atopic dermatitis (AD) and psoriasis. Hereby, NGF was found to interact with the major cellular components (mast cells and eosinophils) of both skin diseases. Objectives: In order to investigate NGF serum levels as a possible clinical marker of disease activity and immunological status, we determined serum NGF, eosinophil cationic protein (ECP), total IgE and score of AD (SCORAD) in 57 patients with AD as well as NGF and the psoriasis area and severity index (PASI) in 17 patients with psoriasis. Fifty healthy subjects served as controls. Methods: We used a highly sensitive improved fluorometric two-site ELISA system for serum NGF detection. IgE and ECP were measured by CAP-FEIA and radioimmunoassay. Results: We did not find a significant correlation between NGF and either ECP, total IgE, or severity of disease assessed by SCORAD. Also in patients with psoriasis, there was no significant correlation with disease activity determined by PASI. Conclusion: Even though there is increasing evidence showing NGF to be involved in the local inflammatory pathophysiology of AD within skin lesions, our findings suggest that NGF systemic serum concentration is not a suitable parameter to estimate the clinical or immunological status of AD or psoriasis patients. This result is inconsistent with some previous studies showing a positive correlation of serum NGF with AD severity, which might be, for example, due to the fact that the present results are corrected for unspecific binding.

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“…In contrast, PGP9.5-positive neurites were abundant in the epidermis of mite-infested Cftr DF508/DF508 mice and NC/Nga mice ( Figure 2a, DF508/DF508 and NC/Nga). 29 The area of intraepidermal neurites was significantly increased in the epidermis of mite-infested Cftr DF508/DF508 mice compared with that of mite-infected CFTR þ / þ mice (Figure 2b). It has been reported that NGF has effects on neurite outgrowth.…”

Section: Increased Scratching Behavior and Skin Fibrotic Phenotypes Imentioning

confidence: 93%

“…Increased Neurite Density and NGF Level in Skin of Mite-Infested Cftr DF508/DF508 Mice Several studies suggest that epidermal neurite outgrowth is related to itching in the atopic skin, 29,30 therefore, we examined whether neurite outgrowth in epidermis occurred in the skin of mite-infested Cftr DF508/DF508 mice at the eighth week of co-housing with skin-lesioned NC/Nga mice. We analyzed mice skin sections using PGP9.5 antibody, which stains sensory nerve endings.…”

Section: Increased Scratching Behavior and Skin Fibrotic Phenotypes Imentioning

confidence: 99%

CFTR-deficiency renders mice highly susceptible to cutaneous symptoms during mite infestation

Hashimoto

Shuto

Mizunoe

et al. 2011

Laboratory Investigation

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Pruritus, also known as itch, is a sensation that causes a desire to scratch. Prolonged scratching exacerbates skin lesions in several skin diseases such as atopic dermatitis. Here, we identify the cystic fibrosis transmembrane conductance regulator (CFTR/Cftr), an integral membrane protein that mediates transepithelial chloride transport, as a determinant factor in mice for the susceptibility to several cutaneous symptoms during mite infestation. Mice that endogenously express dysfunctional Cftr (Cftr DF508/DF508 ) show significant increase of scratching behavior and skin fibrosis after mite exposure. These phenotypes were due to the increased expression of nerve growth factor (NGF) that augments the sensitization of peripheral nerve fibers. Moreover, protein gene product 9.5 (PGP9.5)-positive neurites were abundant in the epidermis of mite-infested Cftr DF508/DF508 mice. Furthermore, mite-infested Cftr þ / þ mice orally administered with a chloride channel inhibitor glibenclamide had higher scratching count and increased level of NGF than vehicle-treated mice. Consistently, mite extract-exposed primary and transformed human keratinocytes, treated with CFTR inhibitor, had significantly higher level of NGF mRNA compared with vehicle-treated, mite extract-exposed cells. These results reveal that CFTR in keratinocytes plays a critical role for the regulation of peripheral nerve function and pruritus sensation, and suggest that Cftr DF508/DF508 mice may serve as a novel mouse model that represents NGF-dependent generation of pruritus.

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Nerve growth factor (NGF) and epidermal nerve fibers in atopic dermatitis model NC/Nga mice

Cited by 28 publications

References 9 publications

Macrophage-Mediated Dorsal Root Ganglion Damage Precedes Altered Nerve Conduction in SIV-Infected Macaques

Macrophage-Mediated Dorsal Root Ganglion Damage Precedes Altered Nerve Conduction in SIV-Infected Macaques

Clinical Relevance of Nerve Growth Factor Serum Levels in Patients with Atopic Dermatitis and Psoriasis

CFTR-deficiency renders mice highly susceptible to cutaneous symptoms during mite infestation

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