Nerve growth factor (NGF) restores depletions of calcitonin gene-related peptide and substance P in sensory neurons from diabetic mice in vitro

Sango, Kazunori; Verdes, José Manuel; Hikawa, Naoshi; Horie, Hidenori; Tanaka, Shun‐ichi; Inoue, Shuji; Sotelo, Julio; Takenaka, Toshifumi

doi:10.1016/0022-510x(94)90087-6

Cited by 49 publications

(24 citation statements)

References 14 publications

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“…Diemel et al [65]showed that NGF administration prevents the alterations in levels of substance P and CGRP in the sciatic nerve and normalises the mRNA levels of γ-preprotachykinin and CGRP in DRG cells in rats. Similar results were obtained by Sango et al [66]who showed that NGF reverses the depletion of substance P and CGRP in sensory neurons in diabetic mice. In a different animal model of peripheral neuropathy using transgenic diabetic mice, Elias et al [67]demonstrated that NGF administration successfully restores C-fibre function.…”

Section: Therapeutic Effects Of Ngf In Experimental Modelssupporting

confidence: 80%

Biological Actions of Nerve Growth Factor in the Peripheral Nervous System

Rask¹

1999

Eur Neurol

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Since the discovery of nerve growth factor (NGF), its role in the physiology/pathophysiology of nerve function has been under intense investigation. More recently, the potential of recombinant human NGF (rhNGF) as a putative treatment for peripheral neuropathies, in particular diabetic polyneuropathy and HIV-associated sensory neuropathy, is being explored. In animal models of diabetes, depletion of endogenous NGF levels has been demonstrated in foot skin and skeletal muscle; these levels reduce further with increasing disease duration. Preclinical studies in animal models of diabetes have shown that administration of NGF can reverse or alleviate impairment in nerve function.

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Section: Therapeutic Effects Of Ngf In Experimental Modelssupporting

confidence: 80%

Biological Actions of Nerve Growth Factor in the Peripheral Nervous System

Rask¹

1999

Eur Neurol

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“…The complex is transported to the DRG or 14, 31 -35 The production of the nociceptive neuropeptides substance P in DRG depends on NGF. 16,17,36 Therefore, we measured NGF levels in the fore-and hindpaw skin as well as in the DRG. Our results showed no decrease in the NGF level of the skin of either paw at either 2 months or 4 months after diabetes induction.…”

Section: Discussionmentioning

confidence: 99%

Spatial Distribution of Nociceptive Neuropeptide and Nerve Growth Factor Depletion in Experimental Diabetic Peripheral Nervous System

et al. 2002

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This study investigated the time-course of the nociceptive neuropeptide substance P and nerve growth factor (NGF), which facilitates substance P production, in lumbar and cervical dorsal root ganglia (DRG) of streptozotocin-induced diabetic rats. Levels of substance P and NGF were measured by radioimmunoassay and sandwich enzyme-linked immunosorbent assay, respectively, 2 months, 4 months and 8 months after induction of diabetes, and compared with age-matched non-diabetic control rats. At 2 months and 4 months, substance P and NGF levels were lower in the lumbar DRG of the diabetic rats than in controls. At 8 months, substance P and NGF were lower in both the lumbar and cervical DRG of the diabetic rats than in controls. These data demonstrate that a decrease in substance P levels in primary sensory neurons with NGF depletion occurs in an axonal length-dependent manner in diabetic rats, and that this decrease may be correlated with the duration of diabetes.

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“…Our hypothesis was that diabetes is associated with neuronal dysfunction, thus we had anticipated that the combination of diabetes and CGRP depletion would have an even greater suppressive effect on insulin-induced vasodilatory response. This is especially true since diabetes alone is associated with a decreased CGRP content in tissue [16,20]. However, this was not the case.…”

Section: Discussionmentioning

confidence: 50%

“…CGRP has been shown to be localized in the perivascular nerves of blood vessels throughout the cardiovascular system [13][14][15]. The CGRP content of vascular neurons is influenced by insulin status where it has been shown to be decreased in diabetes [16]. In other studies CGRP or CGRP agonist have been demonstrated to decrease the tissue responses to insulin [17].…”

Section: Introductionmentioning

confidence: 97%