Nerve growth factor production in sheep antral follicles☆

Mattioli, Mauro; Barboni, Barbara; Gioia, Luisa; Lucidi, Pia

doi:10.1016/s0739-7240(99)00056-9

Cited by 30 publications

(31 citation statements)

References 12 publications

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“…Although we do not have any evidence of the cell types in the ovary involved in the production of NGF in Shiba goats, our present data show that NGF and its receptors (TrkA and p75LNGFR) are expressed in granulosa and theca cells of the ovary. This is in agreement with a previous study demonstrating granulosa and theca cells as a possible source of NGF [18].…”

Section: Discussionsupporting

confidence: 94%

“…This finding is similar to that found in other mammals, such as rats [15], cattle [16], monkeys [17] sheep [18] and mice [19]. In the bovine ovary, NGF, TrkA and p75LNGFR mRNAs are present in theca cells.…”

Section: Discussionsupporting

confidence: 88%

“…In ewes, production of NGF in both in vitro and in vivo is negligible in small follicles and becomes apparent in medium and large follicles. The presence of NGF accumulated in follicular fluid further supports the proposal that NGF may play a role in the control of follicular development [18]. In mice and rats, the mRNA signal for ovarian NGF w a s d e t e c t e d f r o m t h e l a t e s t a g e o f f e t a l development, and decreased postnatally at the time of folliculogenesis.…”

Section: Discussionsupporting

confidence: 65%

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Immunolocalization of Nerve Growth Factor (NGF) and Its Receptors (TrkA and p75LNGFR) in the Reproductive Organs of Shiba Goats

Ren

Medan

Wang

et al. 2005

Journal of Reproduction and Development

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Abstract. The objective of this study was to determine the immunolocalization of NGF and its receptors (TrkA and p75LNGFR) in the reproductive tract of the Japanese Shiba goats. Five adult goats were used in this study and sections of ovaries, uteri and oviducts were immunostained by the avidin-biotin-peroxidase complex method (ABC). The results showed that NGF and its receptors (TrkA and p75LNGFR) were expressed in granulosa cells, theca cells, interstitial cells and lutein cells in ovaries. Immunoreactions for NGF, TrkA and p75LNGFR were also detectable in epithelial cells and muscle cells of the ampulla and isthmus of the oviduct, and in epithelial cells and uterine glands of the uterus. These results strongly suggest autocrine and paracrine regulation of reproductive function by NGF in the reproductive tract of female Shiba goats.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 65%

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Immunolocalization of Nerve Growth Factor (NGF) and Its Receptors (TrkA and p75LNGFR) in the Reproductive Organs of Shiba Goats

Ren

Medan

Wang

et al. 2005

Journal of Reproduction and Development

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“…Western blot analysis also showed that both NGF and TrkA protein levels were higher in the large follicles than in the small or medium follicles. A similar phenomenon was found for NGF production in antral follicles of ewes [20]. The increasing expression of NGF and TrkA in porcine antral follicles, together with the size of these structures, implies that NGF contributes not only to innervation of rapidly growing follicles, but also to steroidogenic cell proliferation, steroid production and follicular vasculature, as has been observed in rodents [16,37,38].…”

supporting

confidence: 72%

Expression of Nerve Growth Factor and Its Receptors, TrkA and p75, in Porcine Ovaries

Jana

Koszykowska

Czarzasta

2011

Journal of Reproduction and Development

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Abstract. The cellular localization of nerve growth factor (NGF) and its receptors (TrkA, p75) was investigated during the estrous cycle in gilts. Also, the levels of expression of these factors in walls of tertiary follicles and corpora lutea (CLs) were determined using Western blot. The ovaries from days 3, 7, 16 and 20 of the cycle revealed the presence of NGF and its receptors in oocytes of secondary and tertiary follicles, follicular cells of primary and secondary follicles, thecal and granulosa cells of tertiary follicles and steroidogenic cells of CLs. In wall cells of primary follicles, NGF, TrkA and p75 staining was strongest on day 16, while in secondary follicles, only p75 was more intensely stained on day 16 and 20. In walls of small (to 3 mm in diameter) and medium (4-6 mm in diameter) follicles, NGF staining was lower on day 16, and the p75 reaction was strongest on day 20. On day 20, NGF staining in large follicles (7-10 mm in diameter) was higher than in smaller follicles. The levels of NGF and p75 in small and medium follicles were highest on day 20. The contents of NGF and TrkA in large follicles on day 20 were higher than in smaller follicles. NGF and TrkA contents in CLs were highest on day 7. Our study demonstrates that NGF, TrkA and p75 are expressed in the ovary during the estrous cycle in gilts. These results suggest that NGF and its receptors may be important for ovarian function in cycling gilts. Key words: Nerve growth factor, Ovary, Pig, p75, TrkA (J. Reprod. Dev. 57: [468][469][470][471][472][473][474] 2011) erve growth factor (NGF) belongs to a family of neurotrophin (NTs) target-derived trophic factors required for survival and differentiation of neuronal populations in both the central and peripheral nervous system. Other NTs have been identified besides NGF, including brain-derived neurotrophic factor (BDNF) [1], , and . NTs bind to two kinds of receptors with dissociation constants of 10 -9 M and 10 -11 M that denominate low-and high-affinity receptors, respectively, as reviewed by Friedman and Greene [5]. The low-affinity receptor is p75, that belongs to the tumor necrosis receptor family and binds all NTs with similar affinity [6]. There are 3 high-affinity receptors, all tyrosine kinase (Trk) receptors: TrkA, TrkB and TrkC. The Trk proteins bind preferentially to specific NTs. TrkA binds NGF, TrkB binds BDNF and NT-4/5 and TrkC binds NT-3 ligands [7,8]. Most of the survival and growth properties elicited by NGF are mediated by TrkA [7]. The p75 can bind to TrkA and enhance responsiveness to NGF. Without TrkA, activation of p75 can induce apoptosis [9].It is very well known that the action of NTs including NGF is not limited to the nervous system: NTs and their receptors have been found in the immune and endocrine systems [8][9][10]. Ovarian cells have been shown to express NGF and/or TrkA and p75 in a variety of mammals, including women [11][12][13], rats [14,15], golden hamsters [16], cows [17,18], sheep [19,20] and Shiba goats [21]. NGF and its receptors regulate severa...

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“…In humans, the ability of hCG to rapidly increase trkA receptor expression in ovarian cell dispersates indicates that a significant fraction of the preovulatory increase in trkA mRNA abundance is an LH-dependent phenomenon [18]. Similarly, the functional relationship between gonadotropins and NGF in studies of the sheep ovary shows that large follicles respond with increased NGF release to in vitro stimulation with a combination of LH and FSH [44]. Taken together, this suggests that the present results are compatible with the broader concept that NGF and its receptors are expressed in ovarian tissues, including interstitial cells, that these expressions are LH-dependent and that NGF and its receptors may contribute to events either leading to or associated with the ovulatory process.…”

Section: Discussionmentioning

confidence: 99%

Immunodetection of NGF, trkA, p75 and Inhibin .ALPHA.-Subunit in Interstitial Cells of Golden Hamsters Treated with hCG

et al. 2009

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Abstract. The aim of this study was to investigate the effect of luteinizing hormone (LH) surge on expression of nerve growth factor (NGF), trkA, p75 and inhibin α-subunit in ovarian interstitial cells of golden hamsters after human chorionic gonadotropin (hCG) treatment. NGF, two NGF receptors (trkA, p75) and inhibin α-subunit were immunolocalized by immunohistochemistry, and gonadotropins, steroid hormones and immunoreactive (ir-) inhibin concentrations were measured by radioimmunoassay. Stronger positive staining of NGF, trkA and p75 were found in interstitial cells at 6, 12 and 18 h after hCG injection in the treated group compared with the control groups. Inhibin α-subunit staining was found in interstitial cells at 12 and 18 h after hCG injection in the treatment group, but not in the control group. Plasma concentrations of progesterone increased significantly from 6 to 18 h after hCG treatment, whereas plasma concentrations of estradiol-17β significantly decreased compared with the control group. An increased plasma concentration of FSH and decreased concentrations of ir-inhibin from 6 to 18 h after hCG treatment verified the negative relationship. There were no significant changes in the concentrations of LH in the hCG-treated group. In contrast, the typical preovulatory LH surge was found at 1700 h on day 4 (proestrus) in the control group. These results suggested that LH surge can induce expression of NGF, trkA, p75 and inhibin α-subunit in ovarian interstitial cells and that NGF, trkA, p75 and inhibin α-subunit may have a paracrine or autocrine role in modulation of ovarian interstitial cell function in golden hamsters. Key words: Golden hamster, Inhibin α-subunit, Interstitial cell, Nerve growth factor (NGF), p75 and trkA (J. Reprod. Dev. 55: [622][623][624][625][626][627][628] 2009) erve growth factor (NGF) belongs to a family of related proteins required for survival, maintenance and development of discrete neuronal populations in the central and peripheral nervous systems [1][2][3]. The effect of NGF has been shown to be mediated through a specific high-affinity membrane receptor, tyrosine kinase A (trkA), that is responsible for NGF's biological activities [4,5]. Furthermore, the effect of NGF is also mediated via low affinity p75 receptor, which is also a receptor of other neurotropins [6]. It is now well known that the mammalian ovary not only contains four of the known neurotropins [7][8][9][10][11][12], but also expresses the receptors for each of them [13,14]. Previous papers have indicated that NGF and its receptors play a critical role in the development of mammalian ovary, oogenesis and folliculogenesis [15][16][17][18][19][20][21]. Inhibin and activins are dimeric glycoproteins formed by two of three different subunits (α, βA and βB). Inhibins consist of either of the β-subunits dimerized with a common α-subunit (α:βA and α:βB; inhibin A and inhibin B, respectively). Activins are dimers of β-subunits (βA:βA, βA:βB and βB:βB; activin A, activin AB and activin B, respectively). Inhibin/...

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Nerve growth factor production in sheep antral follicles☆

Cited by 30 publications

References 12 publications

Immunolocalization of Nerve Growth Factor (NGF) and Its Receptors (TrkA and p75LNGFR) in the Reproductive Organs of Shiba Goats

Immunolocalization of Nerve Growth Factor (NGF) and Its Receptors (TrkA and p75LNGFR) in the Reproductive Organs of Shiba Goats

Expression of Nerve Growth Factor and Its Receptors, TrkA and p75, in Porcine Ovaries

Immunodetection of NGF, trkA, p75 and Inhibin .ALPHA.-Subunit in Interstitial Cells of Golden Hamsters Treated with hCG

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