Nerve growth factor with fibrin glue in end-to-side nerve repair in rats

Silva, Daniel Nunes e; Silva, Andréia Conceição Milan Brochado Antoniolli da; Aydos, Ricardo Dutra; Viterbo, Fausto; Pontes, Elenir Rose Jardim Cury; Odashiro, Danilo N.; Castro, Rafael José de; Augusto, Diogo Gomes

doi:10.1590/s0102-86502012000400008

Cited by 15 publications

(10 citation statements)

References 13 publications

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“…Glues deserve separate considerations; in basic experiences, fibrin glue has been shown to be a good sealant in end-to-end nerve repair [ 98 , 99 , 100 ], and starting from Palazzi’s data [ 98 ], it has also been investigated as an interesting conduit for nerve regeneration, but its role has been questioned as not effective in end-to-side nerve regeneration [ 5 ]; however, these data have been questioned and fibrin’s role reconsidered [ 101 , 102 ].…”

Section: Discussionmentioning

confidence: 99%

Epineurial Window Is More Efficient in Attracting Axons than Simple Coaptation in a Sutureless (Cyanoacrylate-Bound) Model of End-to-Side Nerve Repair in the Rat Upper Limb: Functional and Morphometric Evidences and Review of the Literature

et al. 2016

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End-to-side nerve coaptation brings regenerating axons from the donor to the recipient nerve. Several techniques have been used to perform coaptation: microsurgical sutures with and without opening a window into the epi(peri)neurial connective tissue; among these, window techniques have been proven more effective in inducing axonal regeneration. The authors developed a sutureless model of end-to-side coaptation in the rat upper limb. In 19 adult Wistar rats, the median and the ulnar nerves of the left arm were approached from the axillary region, the median nerve transected and the proximal stump sutured to the pectoral muscle to prevent regeneration. Animals were then randomly divided in two experimental groups (7 animals each, 5 animals acting as control): Group 1: the distal stump of the transected median nerve was fixed to the ulnar nerve by applying cyanoacrylate solution; Group 2: a small epineurial window was opened into the epineurium of the ulnar nerve, caring to avoid damage to the nerve fibres; the distal stump of the transected median nerve was then fixed to the ulnar nerve by applying cyanoacrylate solution. The grasping test for functional evaluation was repeated every 10–11 weeks starting from week-15, up to the sacrifice (week 36). At week 36, the animals were sacrificed and the regenerated nerves harvested and processed for morphological investigations (high-resolution light microscopy as well as stereological and morphometrical analysis). This study shows that a) cyanoacrylate in end-to-side coaptation produces scarless axon regeneration without toxic effects; b) axonal regeneration and myelination occur even without opening an epineurial window, but c) the window is related to a larger number of regenerating fibres, especially myelinated and mature, and better functional outcomes.

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Section: Discussionmentioning

confidence: 99%

Epineurial Window Is More Efficient in Attracting Axons than Simple Coaptation in a Sutureless (Cyanoacrylate-Bound) Model of End-to-Side Nerve Repair in the Rat Upper Limb: Functional and Morphometric Evidences and Review of the Literature

et al. 2016

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“…A case report used fibrin sealant to help seal a dural CSF leak causing a pseudomeningocele following lumbar disc surgery with a subsequent complete resolution of a concomitant abducens nerve palsy and diplopia [228]. Several papers address the role of fibrin sealant in nerve repair including an end to side nerve repair in rats [229] and a facial nerve repair in dogs [230] failing to find a fibrin sealant benefit and a favorable report of using a fibrin conduit for mesenchymal stem cells particularly in the presence of cylcosporine to enhance regeneration following peripheral nerve injury in rats [231]. Laser soldering in pigs [232] was described as superior to fibrin sealant for dural reconstruction as having higher burst pressure strength (98.00 ± 21.41 versus 70.80 ± 15.09 mmHg, resp.…”

Section: Less Frequently Published Clinical Applications Of Fibrinmentioning

confidence: 99%

Fibrin Sealant: The Only Approved Hemostat, Sealant, and Adhesive—a Laboratory and Clinical Perspective

2014

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Background. Fibrin sealant became the first modern era material approved as a hemostat in the United States in 1998. It is the only agent presently approved as a hemostat, sealant, and adhesive by the Food and Drug Administration (FDA). The product is now supplied as patches in addition to the original liquid formulations. Both laboratory and clinical uses of fibrin sealant continue to grow. The new literature on this material also continues to proliferate rapidly (approximately 200 papers/year). Methods. An overview of current fibrin sealant products and their approved uses and a comprehensive PubMed based review of the recent literature (February 2012, through March 2013) on the laboratory and clinical use of fibrin sealant are provided. Product information is organized into sections based on a classification system for commercially available materials. Publications are presented in sections based on both laboratory research and clinical topics are listed in order of decreasing frequency. Results. Fibrin sealant remains useful hemostat, sealant, and adhesive. New formulations and applications continue to be developed. Conclusions. This agent remains clinically important with the recent introduction of new commercially available products. Fibrin sealant has multiple new uses that should result in further improvements in patient care.

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“…The use of the fibular nerve in studies of nerve regeneration was boosted after the reintroduction of the end-to-side neurorrhaphy 1,2,3,4,5,6,7,8,9,10,11,23,13 . We were not able to find many references to the unmyelinated axons of the fibular nerve in these and other recent studies.…”

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confidence: 99%

A histomorphometric study of unmyelinated fibers of the fibular nerve in Wistar rats

Tognon-Miguel

Nascimento-Elias

Schiavoni

et al. 2016

Arq. Neuro-Psiquiatr.

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There are few histomorphometric studies on the unmyelinated fibers of the fibular nerve in rats, and the number of experimental studies using this nerve has been increasing in the last years. Sixty-two percent of the endoneurial area from 10 fibular nerves of adult Wistar rats was scanned by electron microscopy, and digitized. The total number of unmyelinated axons (1.882 ± 271) was significantly lesser, and their axon diameters (0.2 µm to 2.8 µm) significantly higher than that determined in previous studies. The histogram peaked at 1 µm. The differences could be due to the nerve sampled area, the number and the age of the animals evaluated, and the laboratory techniques used. This study brings new and referential data to be used in experimental investigations involving histomorphometric evaluation of the rat fibular nerve.

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Nerve growth factor with fibrin glue in end-to-side nerve repair in rats

Cited by 15 publications

References 13 publications

Epineurial Window Is More Efficient in Attracting Axons than Simple Coaptation in a Sutureless (Cyanoacrylate-Bound) Model of End-to-Side Nerve Repair in the Rat Upper Limb: Functional and Morphometric Evidences and Review of the Literature

Epineurial Window Is More Efficient in Attracting Axons than Simple Coaptation in a Sutureless (Cyanoacrylate-Bound) Model of End-to-Side Nerve Repair in the Rat Upper Limb: Functional and Morphometric Evidences and Review of the Literature

Fibrin Sealant: The Only Approved Hemostat, Sealant, and Adhesive—a Laboratory and Clinical Perspective

A histomorphometric study of unmyelinated fibers of the fibular nerve in Wistar rats

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