Nerve trophic effects: Partial purification from chick embryo brains of proteins that stimulate protein synthesis in cultured newt blastemata

Choo, Alfred F.; Logan, David M.; Rathbone, Michel P.

doi:10.1016/0014-4886(81)90289-2

Cited by 14 publications

(9 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Curiously, the blastema tissue shows a bell-shaped concentration dependence for CBGF activity, whereas 3T3 cells exhibit a more characteristic sigmoidal dependence. Such a bell-shaped concentration dependence was also observed by Choo et al (1978Choo et al ( ,1981 for the stimulation of protein synthesis in cultured blastemas by crude extracts and partially purified fractions from chick brain. This discrepancy in response of the two tissues to increased concentrations of UM-05 retentate material might in fact reflect dissimilarities in culture conditions, the presence of tissue-specific inhibitors, or inherent differences in the cells' responsiveness to the same growth factor.…”

Section: Purification Of Cbgf Activity From Brain Extractssupporting

confidence: 61%

“…Statistical significance of the results was determined using Student's t test for paired observations, Swiss mouse 3T3 fibroblast bioassay. Previous results of Choo et al (1981) raised the possibility that at least one of their partially purified neurotrophic factors for blastema growth was similar in size to the fibroblast growth factors (FGF) first isolated from bovine brain (Gospodarowicz et al, 1978) and pituitary (Gospodarowicz, 1975). Consequently, fractions with activity in our blastema bioassay were simultaneously monitored for their effects on fibroblast growth using a bioassay described by Gospodarowicz et al (1978).…”

Section: Methodsmentioning

confidence: 91%

“…These are based on the ability of test materials to enhance the rates of macromolecular synthesis (Vethamany-Globus et al, 1978;Choo et al, 1981) or mitosis (Vethamany- Globus et al, 1978;Carlone and Foret, 1979) in excised blastemas in organ culture. With this system, Choo et al (1981) were able to characterize at least two proteins with neurotrophic activity from extracts of embryonic 0 1985 ALAN R. LISS, INC chick brain. These factors, basic proteins of relatively low molecular weight, were, however, purified in extremely low yields and were discovered to be quite labile.…”

mentioning

confidence: 99%

“…In the present paper we report the isolation of a lowmolecular-weight peptide from adult chicken brain extracts that is a potent stimulator, at nanomolar concentrations in vitro, of blastema cell DNA synthesis. Moreover, unlike the factors isolated by Choo et al (1981), our neurotrophic activity is extremely heat stabile and can be partially purified in relatively high yield. This factor, which we have tentatively named chick brain growth factor (CBGF), appears to be biochemically distinct from other neural peptides reported to enhance blastema cell proliferation either in vivo or in vitro.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Partial purification of a low‐molecular‐weight growth factor from chicken brain

Carlone

Rathbone

1985

Journal Cellular Physiology

Self Cite

View full text Add to dashboard Cite

The regenerating amphibian limb serves as a useful model for studying factors influencing cell proliferation and differentiation. In particular, peripheral nerves are thought to provide a stimulus for growth of the blastema, presumably via the elaboration of an as yet unidentified neurotrophic factor. In the present study, pressure ultrafiltration coupled with chromatofocusing have proven to be effective methods of partially purifying a neurotrophic factor from adult chicken brains. This chick brain growth factor (CBGF) appears to be a heat-stable, basic peptide of low molecular weight (less than 6,000). It is a potent mitogen in vitro, at nanomolar concentrations, for both blastema cells and Swiss mouse 3T3 fibroblasts. CBGF is apparently distinct from other peptide mitogens and/or neuromodulators that have been reported to stimulate blastema growth in vivo and in vitro. These include substance P, FGF from bovine brain and pituitary, EGF, transferrin (sciatin), and spinal cord growth factor (SCGF). The possible relationship of CBGF to other neural regulatory molecules is discussed.

show abstract

Section: Purification Of Cbgf Activity From Brain Extractssupporting

confidence: 61%

Section: Methodsmentioning

confidence: 91%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Partial purification of a low‐molecular‐weight growth factor from chicken brain

Carlone

Rathbone

1985

Journal Cellular Physiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…First, in vivo bioassays involve injection or infusion of nerve extracts into denervated amputated limb [12,16,19,25]. Second, in vitro bioassays involve cultivation of blastemas in medium supplemented with nerve extracts [7,[9][10][11]21].…”

Section: Introductionmentioning

confidence: 99%

Blastema cell proliferation in vitro: Effects of limb amputation on the mitogenic activity of spinal cord extracts

Boilly

Albert

1988

Biology of the Cell

View full text Add to dashboard Cite

Primary cultures of mesenchymal cells of axolotl limb blastemas provide a very sensitive in vitro bioassay for studying nerve dependence of newt regeneration. These cells can be stimulated by crude spinal cord extracts of non-amputated animals in a dose-dependent manner up to 60 micrograms protein/ml of culture medium; at this concentration the mitotic index is increased 4-fold. Spinal cord extracts of axolotls 14 days after forelimb amputation (i.e., late bud stage) are more efficient in stimulating blastema cell proliferation (+50%) than extracts of axolotls 7 days after forelimb amputation (i.e., early bud stage) or of axolotls without amputation. In a similar manner, spinal cord extracts of young axolotls 14 days after forelimb amputation, are more stimulatory than older axolotls 14 d after forelimb amputation which regenerate only a very small blastema during the same time. It appears that spinal cord mitogenic activity is enhanced after limb amputation, probably in correlation with blastema cell requirements for limb regeneration.

show abstract

Levels of cyclic GMP and cyclic AMP in regenerating forelimbs of adult newts following denervation

et al. 1983

View full text Add to dashboard Cite

The effect of short-term denervation (0, 12, 24, and 72 hours) on the levels of cyclic 3'5'-guanosine monophosphate (cGMP) and cyclic 3'5'-adenosine monophosphate (cAMP) in adult newt (Notophthalmus viridescens) forelimbs at 15, 22, and 35 days of regeneration was investigated. Regenerate blastema and stump cyclic nucleotide levels were compared with those of the contralateral intact forelimb and hindlimb, with levels in the normally regenerating blastema, and with levels measured in the forelimbs of intact, nonoperated animals. Variations in cyclic nucleotide levels occurred according to regeneration stage and tissue type. Changes in level were noted immediately upon denervation and subsequently at other sample times in all regenerate and control series. Parallel fluctuations occurred in regenerate stump and contralateral intact forelimbs. Our results from nonamputated denervated and sham-denervated animals indicate that short-term, denervation-associated cyclic nucleotide fluctuations cannot be attributed solely to the loss of innervation.

show abstract

Nerve trophic effects: Partial purification from chick embryo brains of proteins that stimulate protein synthesis in cultured newt blastemata

Cited by 14 publications

References 27 publications

Partial purification of a low‐molecular‐weight growth factor from chicken brain

Partial purification of a low‐molecular‐weight growth factor from chicken brain

Blastema cell proliferation in vitro: Effects of limb amputation on the mitogenic activity of spinal cord extracts

Levels of cyclic GMP and cyclic AMP in regenerating forelimbs of adult newts following denervation

Contact Info

Product

Resources

About