Nesfatin-1-like peptide is a novel metabolic factor that suppresses feeding, and regulates whole-body energy homeostasis in male Wistar rats

Abstract

Nucleobindin-1 has high sequence similarity to nucleobindin-2, which encodes the anorectic and metabolic peptide, nesfatin-1. We previously reported a nesfatin-1-like peptide (NLP), anorectic in fish and insulinotropic in mice islet beta-like cells. The main objective of this research was to determine whether NLP is a metabolic regulator in male Wistar rats. A single intraperitoneal (IP) injection of NLP (100 μg/kg BW) decreased food intake and increased ambulatory movement, without causing any change in total activity or energy expenditure when compared to saline-treated rats. Continuous subcutaneous infusion of NLP (100 μg/kg BW) using osmotic mini-pumps for 7 days caused a reduction in food intake on days 3 and 4. Similarly, water intake was also reduced for two days (days 3 and 4) with the effect being observed during the dark phase. This was accompanied by an increased RER and energy expenditure. However, decreased whole-body fat oxidation, and total activity were observed during the long-term treatment (7 days). Body weight gain was not significantly different between control and NLP infused rats. The expression of mRNAs encoding adiponectin, resistin, ghrelin, cholecystokinin and uncoupling protein 1 (UCP1) were significantly upregulated, while leptin and peptide YY mRNA expression was downregulated in NLP-treated rats. These findings indicate that administration of NLP at 100 μg/kg BW reduces food intake and modulates whole body energy balance. In summary, NLP is a novel metabolic peptide in rats.

Nesfatin-1 and nesfatin-1-like peptide suppress growth hormone synthesis via the AC/PKA/CREB pathway in mammalian somatotrophs

“…For testing the effects of NESF or NLP on GH, cells at 90% of confluence were used. The growth culture media was supplemented with rat NESF (custom synthesized peptide 6 , > 95% purity, ABGENT, USA) or rat NLP (custom synthesized peptide 7 , > 95% purity, ABGENT) at 0 (Control), 0.001, 0.01, 0.1, 1, 10, 100 or 1000 nM concentration and cells were incubated for 1, 6 or 24 h. The 24 h time point was chosen as previous studies with the same cell lines reported mRNA changes at that time 38 , 39 . After incubation, cells were washed twice with 1X PBS before the collection of total RNA or the protein content, as explained below.…”

The potential role of the novel hypothalamic neuropeptides nesfatin-1, phoenixin, spexin and kisspeptin in the pathogenesis of anxiety and anorexia nervosa

“…During proteolytic processing of NUCB2 two inactive derivatives: nesfatin 2 and 3 are also created (Oh-I et al 2006). Interestingly, its sister protein nucleobindin-1 (NUCB1) is a precursor of another very weakly studied neuropeptide called nesfatin-1-like peptide (NLP) that also has anorexigenic properties in animals (Gawli et al 2017). Nesfatin-1 has a highly conserved molecular structure which is characterized by a distinct sequence homology between mammals including human, and the lower vertebrate species (Gonzales et al 2010).…”

Suppressive action of nesfatin-1 and nesfatin-1-like peptide on cortisol synthesis in adrenal cortex cells

“…The presence of NLP was reported in tissues were NUCB2/NESF-1 was previously identi ed, including pancreas, pituitary, gonads, and gut [12,13]. Similarly, NLP was shown to suppress food intake and modulate the expression of appetite-regulatory hormones in gold sh [13] and rats [14].…”

“…324911] attached to a 27G needle in the lower right quadrant of abdomen to avoid damage to internal organs. The selected dose was validated in a previous study [14]. After 30 min, mice were euthanized, and blood samples and internal organs were collected.…”