Nested polymerase chain reaction for assessing the clinical course of tuberculous meningitis

Takahashi, Teruyuki; Nakayama, Tomohiro; Tamura, Masato; Ogawa, Kazuhiko; Tsuda, Hideo; Morita, Akihiko; Hara, Mayumi; Togo, Masaki; Shiota, Hiroshi; Suzuki, Yuko; Minami, Mayumi; Ishikawa, Hiroshi; Miki, Kenji; Shikata, E.; Takahashi, Shirushi; Kuragano, Takahiro; Matsumoto, Koichí; Sawada, Shigemasa; Mizutani, T.

doi:10.1212/01.wnl.0000162052.13838.b2

Cited by 36 publications

(61 citation statements)

References 7 publications

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“…This study, similar to other studies, showed elevated serum ADA levels in pulmonary TB patients compared to those in normal individuals (7,11,(15)(16)(17)(18)(19)(20)(21)(22)(23); however, ADA level was not found to be a reliable indicator for differentiating between pulmonary TB and other pulmonary infections.…”

Section: Nosupporting

confidence: 89%

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Adenosine Deaminase Level as an Indicator for Differentiating Between Active Pulmonary Tuberculosis Infection and Other Pulmonary Infections

et al. 2012

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Background:In spite of recent advents, and novel improvements in the diagnosis and treatment of pulmonary tuberculosis (TB), definitive diagnosis of pulmonary TB in children is problematic. Therefore, measurement of the serum level of adenosine deaminase (ADA) was later introduced as a helpful marker for the diagnosis of TB. Objectives: The purpose of this study was to determine the usefulness of the ADA test in the diagnosis of pulmonary TB, and compare enzyme activity between patients with pulmonary TB and those with other pulmonary infections. The sampling strategy was randomized, and the design of study was case-control diagnostic descriptive, we used IBM SPSS V-16.0.2 software for analyzing our data. Patients and Methods: This study was performed at the National Research Institute of Tuberculosis and Lung Diseases (NRITLD) in Tehran, IR Iran. In all, 49 children, divided into 3 groups, were examined. Of these, 22 had pulmonary TB, 17 had other pulmonary infections, and the remaining 10 children were normal and were assigned to the control group. Results: Serum ADA levels were higher in the pulmonary TB patients than in the control group (P < 0.05), however, these levels did not differ significantly between TB patients and patients with other pulmonary infections (P = 0.391). Our evaluation revealed that ADA level in TB patients with a positive smear of gastric washing were higher than those in patients with a negative smear (P = 0.006). Conclusions: Similar to the other studies, this study showed higher serum ADA level in pulmonary TB patients than in normal individuals. However, ADA was not found to be a suitable marker for differentiating between pulmonary TB and other pulmonary infections.

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Section: Nosupporting

confidence: 89%

“…In the study that performed by Atalas in 2003, their results suggest that ADA level in pulmonary TB patients is higher than normal population, additionally the ADA levels in patients with tuberculosis pleurisy was higher than patients with other tuberculosis manifestations (15,18).…”

Section: Nomentioning

confidence: 93%

Adenosine Deaminase Level as an Indicator for Differentiating Between Active Pulmonary Tuberculosis Infection and Other Pulmonary Infections

et al. 2012

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“…In the diagnosis of TBM, PCR has been widely used to detect M. tuberculosis DNA in CSF samples as a more rapid, sensitive and specific diagnostic method as compared to the conventional procedures involving acid-fast microscopy and isolation by culture [15]. Although few reports have shown that PCR for TBM is less sensitive than culture, its rapidity helps to ensure timely diagnosis [16].…”

Section: Discussionmentioning

confidence: 99%

Rapid diagnosis of tuberculous meningitis: A comparative evaluation of in-house PCR assays involving three mycobacterial DNA sequences, IS6110, MPB-64 and 65 kDa antigen

Rafi

Venkataswamy

Ravi

et al. 2007

Journal of the Neurological Sciences

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“…A recent example of this method includes the use of an IS6110 uniplex PCR assay that had 98% sensitivity (NPV, 99%) and a specificity of 100% (PPV ϭ 100%) against the "gold standard" of culture (167) and an overall sensitivity of 76.4% (NPV, 59.9%) and specificity of 89.2% (PPV, 94.7%) when clinical TBM was included (166). Several methods of PCR have been evaluated, including quantitative nested real-time PCR (208)(209)(210), the Amplicor Mycobacterium tuberculosis Test (23,24), and a variety of other in-house PCR assays (45,46,51,83,104,111,120,123,138,141,165,189,193). These assays have been evaluated specifically in HIV-coinfected patients (59,180) and also as a means to monitor therapy (180,210).…”

Section: Molecular and Biochemical Analysismentioning

confidence: 99%

Central Nervous System Tuberculosis: Pathogenesis and Clinical Aspects

et al. 2008

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SUMMARY Tuberculosis of the central nervous system (CNS) is a highly devastating form of tuberculosis, which, even in the setting of appropriate antitubercular therapy, leads to unacceptable levels of morbidity and mortality. Despite the development of promising molecular diagnostic techniques, diagnosis of CNS tuberculosis relies largely on microbiological methods that are insensitive, and as such, CNS tuberculosis remains a formidable diagnostic challenge. Insights into the basic neuropathogenesis of Mycobacterium tuberculosis and the development of an appropriate animal model are desperately needed. The optimal regimen and length of treatment are largely unknown, and with the rising incidence of multidrug-resistant strains of M. tuberculosis, the development of well-tolerated and effective antibiotics remains a continued need. While the most widely used vaccine in the world largely targets this manifestation of tuberculosis, the BCG vaccine has not fulfilled the promise of eliminating CNS tuberculosis. We put forth this review to highlight the current understanding of the neuropathogenesis of M. tuberculosis, to discuss certain epidemiological, clinical, diagnostic, and therapeutic aspects of CNS tuberculosis, and also to underscore the many unmet needs in this important field.

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Nested polymerase chain reaction for assessing the clinical course of tuberculous meningitis

Cited by 36 publications

References 7 publications

Adenosine Deaminase Level as an Indicator for Differentiating Between Active Pulmonary Tuberculosis Infection and Other Pulmonary Infections

Adenosine Deaminase Level as an Indicator for Differentiating Between Active Pulmonary Tuberculosis Infection and Other Pulmonary Infections

Rapid diagnosis of tuberculous meningitis: A comparative evaluation of in-house PCR assays involving three mycobacterial DNA sequences, IS6110, MPB-64 and 65 kDa antigen

Central Nervous System Tuberculosis: Pathogenesis and Clinical Aspects

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