Nestin expression in reactive astrocytes following focal cerebral ischemia in rats

Duggal, Neil; Schmidt‐Kastner, Rainald; Hakim, Antoine M.

doi:10.1016/s0006-8993(97)00588-x

Cited by 191 publications

(124 citation statements)

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“…It has been reported that nestin is induced in the reactive astrocyte by ischemia (Lin et al, 1995;Duggal et al, 1997;Li and Chopp, 1999), mechanical injury (Kaya et al, 1999), and glutamate toxicity (Clarke et al, 1994). After focal ischemia, nestin was strongly induced around the infarct region in the acute phase (Duggal et al, 1997), and it was sustained for 28 days after ischemia .…”

Section: Discussionmentioning

confidence: 95%

“…After focal ischemia, nestin was strongly induced around the infarct region in the acute phase (Duggal et al, 1997), and it was sustained for 28 days after ischemia . After transient global ischemia, nestin was expressed in the reactive astrocytes in the hippocampus (Lin et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

“…The expression profile of nestin after brain injury, including ischemia, has been studied intensively. Interestingly, after injury, reactive astrocytes express nestin immunoreactivity (Clarke et al, 1994;Lin et al, 1995;Duggal et al, 1997;Li and Chopp, 1999). Given that SVZ and SGZ astrocytes have been identified as precursor cells Seri et al, 2001), these findings may suggest that reactive *Correspondence to: Dr. Kazuo Kitagawa, Division of Strokology, Departement of Internal Medicine and Therapeutics (A8), Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita City, Osaka 565-0871, Japan.…”

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Differential expression of Musashi1 and nestin in the adult rat hippocampus after ischemia

Yagita

Kitagawa

Sasaki

et al. 2002

J of Neuroscience Research

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Both nestin and the neural RNA-binding protein Musashi1 (Msi1) are expressed in neural stem cells in the subventricular zone. Neurogenesis in the hippocampus has received much attention, so we evaluated the expression of Msi1 and nestin in the adult rat hippocampus after transient forebrain ischemia. Both Msi1 and nestin were induced in the reactive astrocytes after ischemia, especially in the CA1 region, until 35 days after ischemia. Induction of both molecules suggested that reactive astrocytes might have immature characteristics. In the subgranular zone (SGZ) of the hippocampal dentate gyrus, Msi1-positive cells formed clusters after ischemia. These cells were labeled by bromodeoxyuridine (BrdU) but did not express glial fibrillary acidic protein. In contrast, very few nestin-positive cells were labeled by BrdU. Our results suggest that neuronal progenitor cells in the SGZ expressed Msi1 but not nestin. © 2002 Wiley-Liss, Inc.Key words: hippocampus; ischemia; neurogenesis; nestin; Neural stem/progenitor cells remain in the adult mammalian brain (Reynolds and Weiss, 1992;Weiss et al., 1996;Johansson et al., 1999;Doetsch et al., 1999), including human brain (Pincus et al., 1998). Neurogenesis continues throughout life in the two restricted zones, the hippocampal subgranular zone (SGZ; Das, 1965, 1967;Gould et al., 1998;Eriksson et al., 1998) and the rostral migratory stream, where newly generated immature neurons migrate from the anterior subventricular zone (SVZ) into the olfactory bulb (Lois and Alvarez-Buylla, 1994). It has been reported that brain injury stimulates neurogenesis in the SGZ and SVZ. Mechanical injury to the dentate gyrus (Gould and Tanapat, 1997), olfactory bulbectomy (Kirschenbaum et al., 1999), seizure (Parent et al., 1997), and ischemia (Liu et al., 1998;Takagi et al., 1999;Kee et al., 2001;Jin et al., 2001;Yoshimura et al., 2001) can affect cell proliferation in these neurogenic regions. Although in situ detection of neuronal progenitor cells is necessary to understand their response in vivo, no specific markers are available with which to detect progenitor cells.Both Musashi1 (Msi1) and nestin are considered selective markers for neural stem/progenitor cells. Msi1 was primarily isolated as the required molecule for asymmetric division of sensory organ precursor cells in Drosophila (Nakamura et al., 1994). Msi1 posttranscriptionally regulates numb gene expression, which is involved in Notch signaling (Imai et al., 2001). In the adult mammalian brain, Msi1 is present in the ependymal cells, subependymal cells, and astrocytes but not in the microglia, oligodendrocyte, or mature neurons (Sakakibara et al., 1996;Sakakibara and Okano, 1997;Kaneko et al., 2000). We recently demonstrated that the cluster-forming proliferative cells in the SGZ after transient forebrain ischemia and after permanent focal ischemia express Msi1 but not glial fibrillary acidic protein (GFAP; Yagita et al., 2001;Takasawa et al., 2002).Nestin was originally identified as an antigen of monoclonal antibody RAT401, whic...

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Differential expression of Musashi1 and nestin in the adult rat hippocampus after ischemia

Yagita

Kitagawa

Sasaki

et al. 2002

J of Neuroscience Research

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“…22) Nestin is expressed in reactive astrocytes in central nervous system injury, together with other intermediate filaments, vimentin, and glial fibrillary acidic protein, and is important in scar formation/gliosis. 5,6,14) Therefore, nestin is a potential marker for endothelial cells during rapid growth. 22) The present study evaluated the proliferative potential of endothelial cells in CAs and AVMs by immunohistochemical analysis of nestin, and comparison with proliferating cell nuclear antigen T. Shimizu et al…”

Section: Introductionmentioning

confidence: 99%

Nestin Expression in Vascular Malformations: A Novel Marker for Proliferative Endothelium

Shimizu

Sugawara

Tosaka

et al. 2006

Neurol. Med. Chir.(Tokyo)

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Cavernous angiomas (CAs) and arteriovenous malformations (AVMs) sometimes show growth or de novo appearance. Proliferative capacity of the endothelium is evident in such malformations. Intermediate filament nestin is a newly identified marker for proliferative endothelium, which was originally detected in the neuroepithelial stem cells of the embryonal central nervous system. Immunohistochemical analysis of nestin was evaluated as a marker for proliferative capacity of endothelial cells by comparison with proliferating cell nuclear antigen (PCNA). Sixteen of 27 CAs and 13 of 20 AVMs were positive for nestin. Likewise, 12 of 27 CAs and 10 of 20 AVMs were positive for PCNA. Nestin staining was stronger in the typical malformative vessels of CAs than in AVMs, in both the endothelial cells and the interstitial cells. Newly formed endothelial cells expressed nestin strongly in the reactive tissues including organizing or encapsulated hematomas. These results suggest that neovascularization occurs in the process of repeated bleeding and remodeling of hematomas.

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“…For instance, in primitive neuroectodermal tumors, elevated nestin characterizes the most malignant cell type (13). Also, nestin protein is elevated in the infiltrating parts of highly metastatic human glioblastomas and astrocytomas (13)(14)(15)(16) and has been proposed to play a role in tumor invasion in melanomas (17).…”

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Nestin Is a Potential Mediator of Malignancy in Human Neuroblastoma Cells

Thomas

Messam

Spengler

et al. 2004

Journal of Biological Chemistry

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Nestin expression in reactive astrocytes following focal cerebral ischemia in rats

Cited by 191 publications

References 45 publications

Differential expression of Musashi1 and nestin in the adult rat hippocampus after ischemia

Differential expression of Musashi1 and nestin in the adult rat hippocampus after ischemia

Nestin Expression in Vascular Malformations: A Novel Marker for Proliferative Endothelium

Nestin Is a Potential Mediator of Malignancy in Human Neuroblastoma Cells

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