Nestin<sup>+</sup>cells and healing the infarcted heart

Calderone, Angelino

doi:10.1152/ajpheart.00716.2011

Cited by 40 publications

(29 citation statements)

References 66 publications

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“…Interestingly, the aforementioned findings and spatiotemporal patterns of nestin-positive cells are analogous to those described for cells derived from the adventitial fibroblasts following brain infarct (Brierley and Brown 1982). In addition, nestin is induced in fibroblast-like cells under fibrotic conditions or after injury in various tissues including heart and kidney (Calderone 2012;Kishaba et al 2010). Our data raise the possibility that these nestin-positive cells are derived from fibroblasts comprising the vascular adventitia of the larger caliber vessels at this later time point, when the capillary bed is actually being destroyed.…”

Section: Discussionsupporting

confidence: 62%

“…Nevertheless, increasing evidence has identified nestin as an intermediate filament protein that interacts with other cytoskeleton proteins. Nestin is involved in cell division, the structural integrity and mobility of cells and tissue stability (Calderone 2012;Chen et al 2006;Herrmann and Aebi 2000;Wang and Stamenovic 2000). Nestin is also reactivated in response to injury during tissue and wound healing (for a review, see Wiese et al 2004).…”

Section: Discussionmentioning

confidence: 99%

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Characterization of nestin expression and vessel association in the ischemic core following focal cerebral ischemia in rats

et al. 2012

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The present study aimed to provide a detailed characterization of the cellular phenotypes of nestin-positive cells in a rat model of ischemic stroke. Nestin-positive cells included reactive astrocytes in the peri-infarct region. In the ischemic core, in which astrocytes had virtually disappeared, nestin expression was exclusively associated with the vasculature, including the microvasculature and larger caliber vessels. Induction of nestin expression in the ischemic core occurred by 3 days post-ischemia. Nestin expression continued through at least 28 days post-ischemia but the cellular profiles of nestin-positive cells changed over this period. In the ischemic core at day 3, nestin-positive cells frequently had long processes that ran parallel along the longitudinal axis of the vasculature. These cells were highly proliferative and expressed the transcription factor for neural/glial progenitors, Sox9. Based on their morphological characteristics and on a double-labeling study, most nestin-positive cells were clearly distinguishable from vasculature-associated cells including endothelial cells, smooth muscle cells and microglia/macrophages. Immunoelectron microscopic findings demonstrated that most nestin-positive cells lay in the perivascular space and had macrophage-like features, indicating morphological similarity to perivascular macrophages. Nestin expression was still associated with the vasculature 14 days after ischemia but appeared in fibroblast-like cells. Thus, our data indicated that, in the ischemic core, nestin expression was not limited to a progenitor/stem cell population but was induced in the vasculature-associated cells. These cell types included perivascular macrophages and fibroblast-like cells that appeared to undergo dynamic structural changes. These results suggest that nestin facilitates cellular structural remodeling in response to ischemic injury.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Characterization of nestin expression and vessel association in the ischemic core following focal cerebral ischemia in rats

et al. 2012

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“…These PCs likely originate from SGCs [10]. In human and rat trigeminal ganglia SGCs were found positive for nestin, a marker of neuroepithelial stem cells [13][14][15][16][17]. Nestin expression in cells may indicate multi-potentiality and regenerative potential [18].…”

Section: Introductionmentioning

confidence: 99%

Quiescent satellite glial cells of the adult trigeminal ganglion

Rusu¹,

Mănoiu

Mirancea

et al. 2014

Open Medicine

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AbstractSensory ganglia comprise functional units built up by neurons and satellite glial cells (SGCs). In animal species there was proven the presence of neuronoglial progenitor cells in adult samples. Such neural crest-derived progenitors were found in immunohistochemistry (IHC). These findings were not previously documented in transmission electron microscopy (TEM). It was thus aimed to assess in TEM if cells of the human adult trigeminal ganglion indeed have ultrastructural features to qualify for a progenitor, or quiescent phenotype. Trigeminal ganglia were obtained from fifteen adult donor cadavers. In TEM, cells with heterochromatic nuclei, a pancytoplasmic content of free ribosomes, few perinuclear mitochondria, poor developed endoplasmic reticulum, lack of Golgi complexes and membrane trafficking specializations, were found included in the neuronal envelopes built-up by SGCs. The ultrastructural pattern was strongly suggestive for these cells being quiescent progenitors. However, further experiments should correlate the morphologic and immune phenotypes of such cells.

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“…[15][16][17][18][19] Importantly, previous studies have reported that Nestin was up-regulated in the infarcted myocardium within 48 hours, and further indicated that Nestin + cells participate in the wound healing NESTIN + CELLS ENHANCE SURVIVAL OF DOXORUBICIN INDUCED HL-1 CELLS after myocardial injury. 20,21) However, whether Nestin + cells participated in the pathogenesis of the DCM has not been reported yet.…”

mentioning

confidence: 99%

Cardiac Nestin<sup>+</sup> Cells Derived from Early Stage of Dilated Cardiomyopathy Enhanced the Survival of the Doxorubicin-Injured Cardiac Muscle HL-1 Cells

Xie

Liao

et al. 2018

Int. Heart J.

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SummaryDilated cardiomyopathy (DCM), as one of the common cardiomyopathies, is a disease of the heart muscle; however, the etiology and pathogenesis of DCM were still poorly understood. Nestin has been reported a special marker of stem/progenitor cells in various tissues, and the tissue resident Nestin + cells could promote the wound healing and tissue remodeling. However, it remains unclear whether Nestin + cells participate in the protection of cardiomyocytes during the pathogenesis of DCM. Here the model of mice DCM was induced by doxorubicin (DOX) intraperitoneal injection and observed heart failure and ventricular enlargement via echocardiography and histologic analysis, respectively. During DCM pathogenesis, the number of Nestin + cells showed a significant peak on day 6 after DOX treatment, which then gradually decreases to lower than normal levels after day 30 in the total population of the heart. Furthermore, we found that the isolated increased heart-derived Nestin + cells are mesenchymal property and could protect DOX-induced HL-1 cells toxicity in vitro by promoting their proliferation and inhibiting their apoptosis. Collectively, our results showed that Nestin + cells increased during DCM pathogenesis and played an important role in protecting against the DOX-induced HL-1 cells loss via regulating proliferation and apoptosis. Thus, the loss of Nestin + cells might be an etiology to DCM pathogenesis, and these cells could be a promising candidate cell source for study and treatment of DCM patients.(Int Heart J 2018; 59: 180-189)

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Nestin⁺cells and healing the infarcted heart

Abstract: Calderone A. Nestin ϩ cells and healing the infarcted heart.

Cited by 40 publications

References 66 publications

Characterization of nestin expression and vessel association in the ischemic core following focal cerebral ischemia in rats

Characterization of nestin expression and vessel association in the ischemic core following focal cerebral ischemia in rats

Quiescent satellite glial cells of the adult trigeminal ganglion

Cardiac Nestin<sup>+</sup> Cells Derived from Early Stage of Dilated Cardiomyopathy Enhanced the Survival of the Doxorubicin-Injured Cardiac Muscle HL-1 Cells

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Nestin+cells and healing the infarcted heart

Abstract: Calderone A. Nestin ϩ cells and healing the infarcted heart.

Cited by 40 publications

References 66 publications

Characterization of nestin expression and vessel association in the ischemic core following focal cerebral ischemia in rats

Characterization of nestin expression and vessel association in the ischemic core following focal cerebral ischemia in rats

Quiescent satellite glial cells of the adult trigeminal ganglion

Cardiac Nestin<sup>+</sup> Cells Derived from Early Stage of Dilated Cardiomyopathy Enhanced the Survival of the Doxorubicin-Injured Cardiac Muscle HL-1 Cells

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Nestin⁺cells and healing the infarcted heart