Nesting Ecology and Foraging Biology of the Mason Bee<i>Osmia (Helicosmia) latreillei</i>Spinola, 1806 (Hymenoptera: Megachilidae) in Western Algeria

Ouahab, Youssouf; Bendifallah, Leila; Rasmont, Pierre; Hammou, Mohamed Aït

doi:10.1080/0005772x.2020.1854987

Cited by 1 publication

(2 citation statements)

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“…To determine the host plants of bee‐collected pollen, techniques have been developed for preparation and identification of pollen collected from the body surface of bees (Westrich, 1986; Wood & Stuart, 2017), pollen traps (Smart et al, 2017; Stoner et al, 2019), bee nests (Telleria, 1998), the crops of solitary bees (Wilson et al, 2010), and honey (Vorwohl, 1967). Identification of pollen, particularly in bulk samples, has many applications in conservation, ecology, and forensic palynology (Alotaibi et al, 2020; Ouahab et al, 2021; Zhang et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of microscopy and metabarcoding to identify pollen used by the critically endangered rusty patched bumble bee, Bombus affinis

Simanonok

Iwanowicz

Raines

et al. 2022

Insect Conserv Diversity

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1. Taxonomic analysis of pollen collected by bees can provide insights into their host plant use, providing information about the plant species selected for targeted conservation strategies. The two main identification approaches used are morphological analysis of pollen samples affixed to microscope slides (i.e., microscopic palynology) and molecular analysis of samples. Both methods are widely used for freshly collected materials and have been compared in multiple studies, yet their application to archived samples remains to be explored. Archived samples may be particularly useful for the study of rare or protected species, particularly when historical foraging patterns are uncertain.2. We used both methods to analyse pollen collected by the endangered rusty patched bumble bee species, Bombus affinis Cresson, applied to museum-archived specimens. Pollen samples were removed from the corbiculae of bumble bees originally collected in Michigan between 1914 and 1974. Samples included 24 rusty patched bumble bees each with large pollen loads on both corbiculae, allowing for pollen from the same bee to be analysed using both methods.3. DNA metabarcoding detected more plant taxa than light microscopy, and DNA barcoding also had higher taxonomic resolution when compared to taxa determined using light microscopy. In many instances, pollen could only be confidently identified to tribe or family with light microscopy. Discrepancy between methods decreased when taxa identified via DNA metabarcoding were binned into ecologically relevant groups corresponding to those identified using light microscopy. 4. Although binning demonstrated smaller within-method variance, there was still minimal correspondence between the two methods. Results indicate there are benefits and biases unique to each method and highlight the utility of binning taxonomic results to morphological or ecological groupings.

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Section: Introductionmentioning

confidence: 99%

“…Identification of pollen, particularly in bulk samples, has many applications in conservation, ecology, and forensic palynology (Alotaibi et al, 2020;Ouahab et al, 2021;Zhang et al, 2021).…”

Section: Introductionmentioning

confidence: 99%