Nestlet Shredding and Nest Building Tests to Assess Features of Psychiatric Disorders in Mice

Dorninger, Fabian; Zeitler, Gerhard; Berger, Johannes

doi:10.21769/bioprotoc.3863

Cited by 21 publications

(13 citation statements)

References 22 publications

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“…Additionally, the NST is a behavior test to assess psychiatric diseases such as anxiety, compulsive-like behaviors, distress, and pain. [60][61][62] Some studies have identified brain functional deficits and brain damage using the NST. 63,64 Our results suggest that oligonol improves anxiety, compulsive-like behavior, and distress in the AD brain.…”

Section: Discussionmentioning

confidence: 99%

Oligonol ameliorates liver function and brain function in the 5 × FAD mouse model: transcriptional and cellular analysis

Jo,

Arjunan,

Choi

et al. 2023

Food Funct.

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Section: Discussionmentioning

confidence: 99%

Oligonol ameliorates liver function and brain function in the 5 × FAD mouse model: transcriptional and cellular analysis

Jo,

Arjunan,

Choi

et al. 2023

Food Funct.

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“…After 30 min of exploration time, the number of marbles buried were counted and then analyzed to compare between the groups. The marbles were counted as buried if 50% or more were concealed under the bedding [ 45 , 46 , 61 , 62 , 63 , 64 , 65 , 66 ].…”

Section: Methodsmentioning

confidence: 99%

Apigenin Alleviates Autistic-like Stereotyped Repetitive Behaviors and Mitigates Brain Oxidative Stress in Mice

Jayaprakash,

Isaev,

Yang

et al. 2024

Pharmaceuticals

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Studying the involvement of nicotinic acetylcholine receptors (nAChRs), specifically α7-nAChRs, in neuropsychiatric brain disorders such as autism spectrum disorder (ASD) has gained a growing interest. The flavonoid apigenin (APG) has been confirmed in its pharmacological action as a positive allosteric modulator of α7-nAChRs. However, there is no research describing the pharmacological potential of APG in ASD. The aim of this study was to evaluate the effects of the subchronic systemic treatment of APG (10–30 mg/kg) on ASD-like repetitive and compulsive-like behaviors and oxidative stress status in the hippocampus and cerebellum in BTBR mice, utilizing the reference drug aripiprazole (ARP, 1 mg/kg, i.p.). BTBR mice pretreated with APG (20 mg/kg) or ARP (1 mg/g, i.p.) displayed significant improvements in the marble-burying test (MBT), cotton-shredding test (CST), and self-grooming test (SGT) (all p < 0.05). However, a lower dose of APG (10 mg/kg, i.p.) failed to modulate behaviors in the MBT or SGT, but significantly attenuated the increased shredding behaviors in the CST of tested mice. Moreover, APG (10–30 mg/kg, i.p.) and ARP (1 mg/kg) moderated the disturbed levels of oxidative stress by mitigating the levels of catalase (CAT) and superoxide dismutase (SOD) in the hippocampus and cerebellum of treated BTBR mice. In patch clamp studies in hippocampal slices, the potency of choline (a selective agonist of α7-nAChRs) in activating fast inward currents was significantly potentiated following incubation with APG. Moreover, APG markedly potentiated the choline-induced enhancement of spontaneous inhibitory postsynaptic currents. The observed results propose the potential therapeutic use of APG in the management of ASD. However, further preclinical investigations in additional models and different rodent species are still needed to confirm the potential relevance of the therapeutic use of APG in ASD.

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“…In order to assess the extent to which pain sensitivity could be obfuscated by debilitating effects of late-stage tumor growth, we employed a nestlet shredding assay [ 22 ], a well-described indicator of well-being and a physiological behavior in which healthy mice are motivated to engage [ 23 , 24 ]. 24 h before testing began, nesting material was removed from the home cages.…”

Section: Methodsmentioning

confidence: 99%

Sensory neuron dysfunction in orthotopic mouse models of colon cancer

Balogh

Zhang

Gaffney

et al. 2022

J Neuroinflammation

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Reports of neurological sequelae related to colon cancer are largely restricted to rare instances of paraneoplastic syndromes, due to autoimmune reactions. Systemic inflammation associated with tumor development influences sensory neuron function in other disease models, though the extent to which this occurs in colorectal cancer is unknown. We induced orthotopic colorectal cancer via orthotopic injection of two colorectal cancer cell lines (MC38 and CT26) in two different mouse strains (C57BL/6 and Balb/c, respectively). Behavioral tests of pain sensitivity and activity did not detect significant alterations in sensory sensitivity or diminished well-being throughout tumor development. However, immunohistochemistry revealed widespread reductions in intraepidermal nerve fiber density in the skin of tumor-bearing mice. Though loss of nerve fiber density was not associated with increased expression of cell injury markers in dorsal root ganglia, lumbar dorsal root ganglia neurons of tumor-bearing animals showed deficits in mitochondrial function. These neurons also had reduced cytosolic calcium levels in live-cell imaging and reduced spontaneous activity in multi-electrode array analysis. Bulk RNA sequencing of DRGs from tumor-bearing mice detected activation of gene expression pathways associated with elevated cytokine and chemokine signaling, including CXCL10. This is consistent with the detection of CXCL10 (and numerous other cytokines, chemokines and growth factors) in MC38 and CT26 cell-conditioned media, and the serum of tumor-bearing mice. Our study demonstrates in a pre-clinical setting that colon cancer is associated with latent sensory neuron dysfunction and implicates cytokine/chemokine signaling in this process. These findings may have implications for determining risk factors and treatment responsiveness related to neuropathy in colorectal cancer.

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Nestlet Shredding and Nest Building Tests to Assess Features of Psychiatric Disorders in Mice

Cited by 21 publications

References 22 publications

Oligonol ameliorates liver function and brain function in the 5 × FAD mouse model: transcriptional and cellular analysis

Oligonol ameliorates liver function and brain function in the 5 × FAD mouse model: transcriptional and cellular analysis

Apigenin Alleviates Autistic-like Stereotyped Repetitive Behaviors and Mitigates Brain Oxidative Stress in Mice

Sensory neuron dysfunction in orthotopic mouse models of colon cancer

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