Net uptake of rat serum protein by Sf &gt; 400 lymph chylomicrons in vitro

Lossow, W.J.; Lindgren, Frank T.; Jensen, L. C.

doi:10.1016/0005-2760(67)90056-2

Cited by 19 publications

(6 citation statements)

References 21 publications

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“…This applies also to lipoproteins of Sf 12-400. This layer is probably a mixed film consisting mainly of phospholipid but also some cholesterol and protein which gives a more compact film [Zilversmit, 1965;van Deenen et al, 1962;Demel et al, 1967;Lossow et al, 1967]. The actual area occupied by each phospholipid molecule is probably somewhat less than 65 A2, since the protein might occupy 10 per cent [Zilversmit, 1965] or 27 per cent [Lossow et al, 1967] of the surface area.…”

Section: Discussionmentioning

confidence: 99%

“…This layer is probably a mixed film consisting mainly of phospholipid but also some cholesterol and protein which gives a more compact film [Zilversmit, 1965;van Deenen et al, 1962;Demel et al, 1967;Lossow et al, 1967]. The actual area occupied by each phospholipid molecule is probably somewhat less than 65 A2, since the protein might occupy 10 per cent [Zilversmit, 1965] or 27 per cent [Lossow et al, 1967] of the surface area. The micellar theory of fat absorption suggests that there is a passive diffusion of micelles of fatty acids, mono-and di-glycerides into the cell and then an active synthesis of triglyceride, phospholipid and protein by the endoplasmic reticulum or Golgi complex to form chylomicrons [Senior and Isselbacher, 1962;Sjostrand, 1963;Hofmann and Borgstrom, 1964;Cardell et al, 1965;Strauss, 1966;Dobbins, 1966].…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Dietary Fat Load on the Size and Composition of Chylomicrons in Thoracic Duct Lymph

1968

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Chylomicrons of Sf 〉 400 and lipoproteins of Sf 12‐400 were separated by ultra‐centrifugation from samples of thoracic duct lymph collected from rabbits fed for 1 to 2 weeks previously on diets containing 5 per cent and 30 per cent corn oil or butter and from rats given a single intragastric dose of 0·5 ml. corn oil. The size of these particles after corn oil was determined by electron microscopy following fixation in osmium tetroxide. In rabbits on the low fat diet the mean diameter of chylomicrons was 960 Å with half the triglyceride in particles of diameter greater than 1400 Å; on the high fat diet the mean diameter was 1435 Å with half the triglyceride in particles of diameter greater than 2700 Å. In rats the mean chylomicron diameter was 1094 Å in the early phase of fat absorption with half the triglyceride in particles of diameter greater than 1320 Å. These figures increased to 2105 Å and 3600 Å respectively at the peak of fat absorption. The relationship between the chemical composition of these complexes and their actual size suggests that phospholipid is uniformly distributed on the surface, each phospholipid molecule occupying approximately 65 Å2. Although meaningful electron micrographs of butter chylomicrons could not be obtained, determinations of the triglyceride : phospholipid ratios indicate that, as with the corn oil‐fed animals, the chylomicrons from the rabbits fed 30 per cent butter are on the average larger than those from the animals fed 5 per cent butter.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Effect of Dietary Fat Load on the Size and Composition of Chylomicrons in Thoracic Duct Lymph

1968

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“…It should be pointed out that the amount of protein on rat chylomicrons isolated from lymph is about one-half of that isolated from serum (21). Presumably, chylomicrons are synthesized in the intestine, released into the lymphatics, and eventtlally reach the systemic circulation (2), at which time they acquire additional protein.…”

Section: Structure and Compositionmentioning

confidence: 99%

Circulating Lipoproteins

Schumaker¹,

Adams²

1969

Annu. Rev. Biochem.

132

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“…Since this protein is functionally active only in triglyceride-rich lipoproteins and evidently transfers readily from HDL to phospholipid-stabilized fat emulsions (8)(9)(10), HDL could provide a source of activator proteins to newly secreted triglyceride-rich lipoproteins. Studies in rats have shown that protein is transferred to lymph chylomicrons when they are exposed to HDL in vitro (11,12).…”

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confidence: 99%

Interchange of Apolipoproteins between Chylomicrons and High Density Lipoproteins during Alimentary Lipemia in Man

Havel¹,

Kane²,

Kashyap³

1973

J. Clin. Invest.

508

133

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A B S T R A C T Apolipoproteins of the "C" group in human blood plasma, which contain the activator of the lipoprotein lipase-substrate interaction, were found to be transferred specifically from serum to phospholipid-stabilized fat emulsion. Content and distribution of apoprotein activator were measured in healthy men in the postabsorptive state and 4 h after ingestion of meals containing 100 g fat. Content of activator protein in whole serum did not change after ingestion of the fat-rich meals but that contained in triglyceride-rich lipoproteins of density (d) < 1.006 approximately doubled whereas that of high density lipoproteins fell by half. The increased activator content of triglyceride-rich lipoproteins was virtually confined to chylomicrons and its concentration in chylomicron apoprotein was substantially greater than that in very low density lipoproteins. This difference could be ascribed largely to a higher content of C apoproteins in chylomicron protein since both the concentration of C apoproteins and of apoprotein activator were directly proportional to particle diameter while the pattern of fast-migrating C apoproteins in polyacrylamide gels was similar among chylomicrons and subfractions of very low density lipoproteins. Apparent concentration of activator protein was much greater in the high density lipoprotein subfraction of d 1.063-1.125 than in the subfraction of d 1.125-1.21. In the subfraction of d 1.063-1.125, the concentration of activator protein and of fast-migrating C

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Net uptake of rat serum protein by Sf > 400 lymph chylomicrons in vitro

Cited by 19 publications

References 21 publications

The Effect of Dietary Fat Load on the Size and Composition of Chylomicrons in Thoracic Duct Lymph

The Effect of Dietary Fat Load on the Size and Composition of Chylomicrons in Thoracic Duct Lymph

Circulating Lipoproteins

Interchange of Apolipoproteins between Chylomicrons and High Density Lipoproteins during Alimentary Lipemia in Man

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