In Brazilian regional landscapes, the Cerrado has one of the richest flora among the savannas in the world, with a high level of endemism; however, many plant species are threatened with extinction as a consequence of spatio-temporal changes in land use and cover. This study aimed to analyze changes in land use and cover in the upper course of the Ceroula stream basin, located in the Cerrado of Mato Grosso do Sul, Brazil, based on maps of land use and cover in 1985 and 2022, the normalized difference vegetation index (NDVI), precipitation data, and fieldwork. The results indicated that in 1985, forest vegetation was replaced by pasture, and in 2022, in addition to pasture, there was the introduction of soybean monoculture with corn in the off-season, influenced by the international commodities market. These land use and cover alterations, without adequate management and in the absence of conservation practices, led to environmental impacts, such as accelerated linear erosive processes (rill, ravine, and gully). The results may help provide important insights into the dynamics of land use and cover, the consequences of the lack of conservation practices, and the environmental impacts in the Cerrado of Mato Grosso do Sul, contributing to better understanding of the environmental challenges faced in the region and the need to provide subsidies for the development of sustainable management strategies.