Network analysis of plasma and tissue amino acids and the generation of an amino index for potential diagnostic use

Noguchi, Yasushi; Zhang, Qingwei; Sugimoto, Tatsuho; Furuhata, Yasufumi; Sakai, Ryosei; Mori, Masato; Takahashi, Mitsuo; Kimura, Takeshi

doi:10.1093/ajcn/83.2.513s

Cited by 137 publications

(102 citation statements)

References 39 publications

Supporting

Mentioning

100

Contrasting

Order By: Relevance

“…Amino acid content was determined with an L-8800 amino acid analyzer (Hitachi). Briefly, amino acids separated by cation-exchange chromatography were detected spectrophotometrically after post-column reaction with ninhydrin reagent (Noguchi et al 2006). …”

Section: Measurement Of Amino Acid Contentmentioning

confidence: 99%

Reproductive organs regulate leaf nitrogen metabolism mediated by cytokinin signal

Igarashi

Izumi

Dokiya

et al. 2008

Planta

View full text Add to dashboard Cite

The metabolism of vegetative organs in plants changes during the development of the reproductive organs. The regulation of this metabolism is important in the control of crop productivity. However, the complexity of the regulatory systems makes it difficult to elucidate their mechanisms. To examine these mechanisms, we constructed model experiments using Arabidopsis to analyze metabolic and gene expression changes during leaf-stage progression and after removal of the reproductive organs. Leaf gene expression levels and content of major amino acids, both of which decreased during leaf-stage progression, increased after removal of the reproductive organs. In particular, the levels of expression of cytokinin biosynthesis genes and cytokinin-responsive genes and the cytokinin content increased after removal of the reproductive organs. Analysis of plants with knockout of a cytokinin-biosynthetic gene (AtIPT3) and a cytokinin receptor gene (AHK3) indicated that glutamate dehydrogenase genes (GDH3) were regulated by cytokinin signaling. These data suggest that cytokinins regulate communication between reproductive and vegetative organs, and that GDH3 is one target of the cytokinin-mediated regulation of nitrogen metabolism.

show abstract

Section: Measurement Of Amino Acid Contentmentioning

confidence: 99%

Reproductive organs regulate leaf nitrogen metabolism mediated by cytokinin signal

Igarashi

Izumi

Dokiya

et al. 2008

Planta

View full text Add to dashboard Cite

show abstract

“…For example, one S-nitrosated target is arginase 1 (Entrez Gene ID: 11846), which is responsible for hydrolyzing L-arginine to form L-ornithine and urea, is involved in the nitrogen metabolism pathway and urea cycle. There are also reports that amino acid metabolism was abnormal in diabetes (Wijekoon et al, 2004;Noguchi et al, 2006). Excess amino acids in the liver can act as substrates for gluconeogenesis (Felig,1975), interfere with the inhibitory effect of insulin on glucose production (Boden and Tappy, 1990), and change the insulin/glucagon ratio (Roden et al, 1996), all of which may increase the output of hepatic glucose.…”

Section: Discussionmentioning

confidence: 99%

Quantitative proteomic analysis of S-nitrosated proteins in diabetic mouse liver with ICAT switch method

et al. 2010

View full text Add to dashboard Cite

In this study we developed a quantitative proteomic method named ICAT switch by introducing isotope-coded affinity tag (ICAT) reagents into the biotin-switch method, and used it to investigate S-nitrosation in the liver of normal control C57BL/6J mice and type 2 diabetic KK-Ay mice. We got fifty-eight S-nitrosated peptides with quantitative information in our research, among which thirty-seven had changed S-nitrosation levels in diabetic mouse liver. The S-nitrosated peptides belonged to fortyeight proteins (twenty-eight were new S-nitrosated proteins), some of which were new targets of S-nitrosation and known to be related with diabetes. S-nitrosation patterns were different between diabetic and normal mice. Gene ontology enrichment results suggested that S-nitrosated proteins are more abundant in amino acid metabolic processes. The network constructed for Snitrosated proteins by text-mining technology provided clues about the relationship between S-nitrosation and type 2 diabetes. Our work provides a new approach for quantifying S-nitrosated proteins and suggests that the integrative functions of S-nitrosation may take part in pathophysiological processes of type 2 diabetes.

show abstract

“…6,7) Recently, among the available focused metabolomics approaches, amino acid metabolomics analysis, which focuses on amino acids as key metabolites, such as aminoindex 8,9) or chiral amino acid metabolomics, 10-12) has received considerable attention because of its utility in the diagnosis and monitoring of pathological conditions. Exogenous and endogenous amino acids are hub substances in regulated metabolic networks, and are also relatively abundant in many biological samples.…”

Section: Abstract Amino Acid Metabolomics; Pancreatic Cancer Cell; Gmentioning

confidence: 99%

Assessment of Anticancer Drug Effects on Pancreatic Cancer Cells under Glucose-Depleted Conditions Using Intracellular and Extracellular Amino Acid Metabolomics

Tomita

Todoroki

Hayama

et al. 2018

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Previously, we developed a method to evaluate states of cells treated with anticancer drugs via the comprehensive analysis of amino acids, termed amino acid metabolomics. In the present study, we evaluated the effects of the anticancer drugs, gemcitabine hydrochloride and pyrvinium pamoate, on the proliferation of a pancreatic cancer cell line (PANC-1) under hypoglycemic conditions using amino acid metabolomics. Intracellular and extracellular amino acid profiles of PANC-1 were determined by hydrophilic interaction chromatography-tandem mass spectrometry with simple pretreatment. Changes to the drugs' anticancer effects resulting from glucose starvation conditions were presented in score plots obtained from principal component analyses. In particular, the analysis of intracellular amino acids was found to be the superior approach because the results allowed a clearer assessment of the cell state. Further, orthogonal partial least squares discriminant analysis was performed to search for amino acid candidates that discriminate with anticancer drug-treated PANC-1 cells. We identified several amino acids that might be able to distinguish the drugtreated group from the control group. These results might provide a better understanding of the mechanisms underlying cell responses such as drug resistance or austerity. The present study is the first to evaluate the efficacy of anticancer drugs under glucose starvation based on the analysis of the variation of extracellular and intracellular amino acid profiles in vitro. Key words amino acid metabolomics; pancreatic cancer cell; glucose depletion; biomarker candidateMetabolomics is a comprehensive analysis of numerous small endogenous molecules contained in cells, biological fluids, and tissues. In addition to a complementary understanding of physiological functions, metabolomics is also a promising tool to explore potential biomarkers for diagnosis, prognosis, and therapeutic response.1) It has proven to be very useful not only in medical and life sciences, but also in clinical research, 2,3) drug discovery, 4) environmental toxicology, 5) and food science. 6,7) Recently, among the available focused metabolomics approaches, amino acid metabolomics analysis, which focuses on amino acids as key metabolites, such as aminoindex 8,9) or chiral amino acid metabolomics, 10-12) has received considerable attention because of its utility in the diagnosis and monitoring of pathological conditions. Exogenous and endogenous amino acids are hub substances in regulated metabolic networks, and are also relatively abundant in many biological samples. Therefore, they are thought to have superior properties as targets of focused metabolomics. In several studies, alterations in the free amino acid profile were found to be related to various disease states, such as cancer. 13,14) Previously, we evaluated the effect of several anticancer agents on cancer cell proliferation using amino acid metabolomics analysis of the culture medium. Amino acid measurement was performed using fluorescence der...

show abstract

Network analysis of plasma and tissue amino acids and the generation of an amino index for potential diagnostic use

Cited by 137 publications

References 39 publications

Reproductive organs regulate leaf nitrogen metabolism mediated by cytokinin signal

Reproductive organs regulate leaf nitrogen metabolism mediated by cytokinin signal

Quantitative proteomic analysis of S-nitrosated proteins in diabetic mouse liver with ICAT switch method

Assessment of Anticancer Drug Effects on Pancreatic Cancer Cells under Glucose-Depleted Conditions Using Intracellular and Extracellular Amino Acid Metabolomics

Contact Info

Product

Resources

About