Network formation through active migration of human vascular endothelial cells in a multilayered skeletal myoblast sheet

Nagamori, Eiji; Ngo, Trung Xuan; Takezawa, Yasunori; Saito, Atsuhiro; Sawa, Yoshiki; Shimizu, Tatsuya; Okano, Teruo; Taya, Masahito; Kino‐oka, Masahiro

doi:10.1016/j.biomaterials.2012.08.055

Cited by 44 publications

(34 citation statements)

References 22 publications

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“…This result implied that the hMSCs sheets provide a 3D environment for HUVECs growth and migration for network formation in all directions. Similar results can be found in Nagamori's study, which reported that HUVECs underneath a multilayered skeletal myoblast sheet actively and vertically migrated into the inner portions of the sheet [31]. The mechanism is still unknown.…”

Section: Discussionsupporting

confidence: 84%

Preparation of Three-Dimensional Vascularized MSC Cell Sheet Constructs for Tissue Regeneration

Liu

et al. 2014

BioMed Research International

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Engineering three-dimensional (3D) vascularized constructs remains a challenge due to the inability to form rich microvessel networks. In this study we engineered a prevascularized 3D cell sheet construct for tissue regeneration using human bone marrow-derived mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells as cell sources. hMSCs were cultured to form a thick cell sheet, and human umbilical vein endothelial cells (HUVECs) were then seeded on the hMSCs sheet to form networks. The single prevascularized HUVEC/hMSC cell sheet was folded to form a 3D construct by a modified cell sheet engineering technique. In vitro results indicated that the hMSCs cell sheet promoted the HUVECs cell migration to form networks in horizontal and vertical directions. In vivo results showed that many blood vessels grew into the 3D HUVEC/hMSC cell sheet constructs after implanted in the subcutaneous pocket of immunodeficient mice. The density of blood vessels in the prevascularized constructs was higher than that in the nonprevascularized constructs. Immunohistochemistry staining further showed that in vitro preformed human capillaries in the prevascularized constructs anastomosed with the host vasculature to form functional blood vessels. These results suggest the promising potential of this 3D prevascularized construct using hMSCs cell sheet as a platform for wide applications in engineering vascularized tissues.

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Section: Discussionsupporting

confidence: 84%

Preparation of Three-Dimensional Vascularized MSC Cell Sheet Constructs for Tissue Regeneration

Liu

et al. 2014

BioMed Research International

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“…The long time to formation (~35 days) and challenges with scale-up have limited utilization of this technique compared to use of natural or synthetic scaffolds. Alternatively, self-assembly of large-area muscle constructs has been achieved using a cell sheet engineering technique in which mixtures of muscle cells and fibroblasts were cultured on dishes coated with thermoresponsive polymer poly(N-isopropylacrylamide) until they generated sufficient ECM and then detached from tissue culture plates by decreasing temperature [218–220]. Stacking of the sheets can allow generation of thicker muscle tissues and incorporation of endothelial and neuronal layers [218, 219]; however, functionality of these muscle constructs has yet to be reported.…”

Section: Therapies For Striated Muscle Disordersmentioning

confidence: 99%

Striated muscle function, regeneration, and repair

Shadrin

Khodabukus

2016

Cell. Mol. Life Sci.

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As the only striated muscle tissues in the body, skeletal and cardiac muscle share numerous structural and functional characteristics, while exhibiting vastly different size and regenerative potential. Healthy skeletal muscle harbors a robust regenerative response that becomes inadequate after large muscle loss or in degenerative pathologies and aging. In contrast, the mammalian heart loses its regenerative capacity shortly after birth, leaving it susceptible to permanent damage by acute injury or chronic disease. In this review, we compare and contrast the physiology and regenerative potential of native skeletal and cardiac muscles, mechanisms underlying striated muscle dysfunction, and bioengineering strategies to treat muscle disorders. We focus on different sources for cellular therapy, biomaterials to augment the endogenous regenerative response, and progress in engineering and application of mature striated muscle tissues in vitro and in vivo. Finally, we discuss the challenges and perspectives in translating muscle bioengineering strategies to clinical practice.

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“…Other tissue engineering approaches such as the use of soft hydrogels, the combination of endothelial cell sheets with myoblast cell sheets, and microfabrication techniques such as 3D printing may be more suitable to obtain aligned myofibers. 7,[9][10][11][12][13] Engineering muscle precursor cells in a soft hydrogel such as fibrin allows the myofibers to align in one direction while fibrin behaves as a proangiogenic, making it a good scaffold for both the formation of myofibers and endothelial networks.…”

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confidence: 99%