Network Pharmacology Combined With Chromatogram Similarity Analysis for Biomarkers Exploration of Duliang Herbal Formulation

Abstract

Objectives: To explore potential biomarkers of the Duliang herbal formula (DHF). Methods: The DHF consisting of a radix of A.dahurica and a rhizome of L. chuanxiong was investigated the pharmacological mechanism for migraine treatment via a network pharmacology approach using published databases and software. Besides, an experimentally chromatographic analysis of DHF was performed to identify potential active components. A combination of two approach results could contribute to propose biomarkers for quality control of DHF. Results: To extend our previous in silico study on anti-migraine compounds in DHF via network pharmacology approach, chromatogram similarity analysis of DHF with different origin herbs had carried out and showed that these active compounds, namely ferulic acid, senkyunolide A, senkyunolide I, ligustilide, byakangelicol, oxypeucedanin, imperatorin, isoimperatorin, and oxypeucedanin hydrate, could be considered as quality markers of formula. Conclusion: Potential biomarkers of DHF for anti-migraine can be explored via an integrated approach using network pharmacology and chromatographic analysis.