Network Pharmacology Identifies the Mechanisms of Action of Tongxie Anchang Decoction in the Treatment of Irritable Bowel Syndrome with Diarrhea Predominant

Abstract

Aim. This study aims to uncover the pharmacological mechanism of Tongxie Anchang Decoction (TXACD), a new and effective traditional Chinese medicine (TCM) prescription, for treating irritable bowel syndrome with diarrhea predominant (IBS-D) using network pharmacology. Methods. The active compounds and putative targets of TXACD were retrieved from TCMSP database and published literature; related target genes of IBS-D were retrieved from GeneCards; PPI network of the common target hub gene was constructed by STRING. Furthermore, these hub genes were analyzed using gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Results. A total of 54 active compounds and 639 targets were identified through a database search. The compound-target network was constructed, and the key compounds were screened out according to the degree. By using the PPI and GO and KEGG enrichment analyses, the pharmacological mechanism network of TXACD in the treatment of IBS-D was constructed. Conclusions. This study revealed the possible mechanisms by which TXACD treatment alleviated IBS-D involvement in the modulation of multiple targets and multiple pathways, including the immune regulation, inflammatory response, and oxidative stress. These findings provide novel insights into the regulatory role of TXACD in the prevention and treatment of IBS-D and hold promise for herb-based complementary and alternative therapy.

Irritable bowel syndrome: Epidemiology, overlap disorders, pathophysiology and treatment

“…And the results reported that the flavored Tongxie Yaofang had a significant effect on the symptoms of patients with IBS-D[ 117 ], and improving the gut microbiome, alleviating visceral hypersensitivity, regulating 5-HT level in patients, and inhibiting colonic contraction are mechanisms for the treatment of IBS[ 118 - 120 ]. Moreover, Tongxie Anchang Decoction improves IBS by reducing visceral hypersensitivity, reversing mast cell infiltration, and regulating 5-HT[ 118 , 121 ]. And Xiang Sha Liu Jun Zi Decoction reduced the mean diarrhea score of IBS patients[ 122 ].…”