Network Pharmacology in Research of Chinese Medicine Formula: Methodology, Application and Prospective

Luo, Ting-Ting; Lu, Yong; Yan, Shaomin; Xue, Xiao; Rong, Xianglu; Guo, Jiao

doi:10.1007/s11655-019-3064-0

Cited by 510 publications

(338 citation statements)

References 94 publications

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“…Network pharmacology has been applied in foreseeing the pharmacological mechanisms of TCM [31][32][33]. Although predictable mechanisms could be obtained from published reports, network pharmacology helps to elucidate the action mechanism of TCM at molecular level with a systematic viewpoint [34]. Therefore, it has been a promising holistic strategy for TCM research.…”

Section: Discussionmentioning

confidence: 99%

Investigation of cardiovascular protective effect of Shenmai injection by network pharmacology and pharmacological evaluation

Lin

Yang

et al. 2020

BMC Complement Med Ther

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Background: Shenmai injection (SMI) has been used in the treatment of cardiovascular disease (CVD), such as heart failure, myocardial ischemia and coronary heart disease. It has been found to have efficacy on doxorubicin (DOX)induced cardiomyopathy. The aims of this study were to explore the underlying molecular mechanisms of SMI treatment on CVD by using network pharmacology and its protective effect on DOX-induced cardiotoxicity by in vitro and in vivo experiment based on network pharmacology prediction.Methods: Network pharmacology method was used to reveal the relationship between ingredient-target-disease and function-pathway of SMI on the treatment of CVD. Chemical ingredients of SMI were collected form TCMSP, BATMAN-TCM and HIT Database. Drugbank, DisGeNET and OMIM Database were used to obtain potential targets for CVD. Networks were visualized utilizing Cytoscape software, and the enrichment analysis was performed using IPA system. Finally, cardioprotective effects and predictive mechanism confirmation of SMI were investigated in H9c2 rat cardiomyocytes and DOX-injured C57BL/6 mice.Results: An ingredient-target-disease & function-pathway network demonstrated that 28 ingredients derived from SMI modulated 132 common targets shared by SMI and CVD. The analysis of diseases & functions, top pathways and upstream regulators indicated that the cardioprotective effects of SMI might be associated with 28 potential ingredients, which regulated the 132 targets in cardiovascular disease through regulation of G protein-coupled receptor signaling. In DOX-injured H9c2 cardiomyocytes, SMI increased cardiomyocytes viability, prevented cell apoptosis and increased PI3K and p-Akt expression. This protective effect was markedly weakened by PI3K inhibitor LY294002. In DOX-treated mice, SMI treatment improved cardiac function, including enhancement of ejection fraction and fractional shortening. Conclusions: Collectively, the protective effects of SMI on DOX-induced cardiotoxicity are possibly related to the activation of the PI3K/Akt pathway, as the downstream of G protein-coupled receptor signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Investigation of cardiovascular protective effect of Shenmai injection by network pharmacology and pharmacological evaluation

Lin

Yang

et al. 2020

BMC Complement Med Ther

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“…In our study, we found the molecular mechanism of BHD's neuroprotection effect against IS using network pharmacology strategy. The network pharmacology strategy is helpful to clarify the mechanism of TCM's function from a systematic viewpoint [40][41]. Furthermore, this method provide a multidimensional research strategy for a complicated decoction.…”

Section: Discussionmentioning

confidence: 99%

Network pharmacology and molecular docking analysis on molecular targets and mechanisms of Buyang Huanwu Decoction in the treatment of Ischemic Stroke

Gao

Tian

Han

et al. 2020

Preprint

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Background and objective: With the exact clinical efficacy, Buyang Huanwu decoction (BHD) is a classical prescription for the treatment of ischemic stroke (IS). Here, we aimed to investigate the pharmacological mechanisms of BHD in treating IS using systems biology approaches.Methods: The bioactive components and potential targets of BHD were screened by TCMSP, BATMAN-TCM, ETCM, and SymMap databases. Besides, compounds that failed to find the targets from the above databases were predicted through STITCH, SWISS, and SEA. Moreover, six databases were searched to mine targets of IS. The intersection targets were obtained, and analyzed by GO and KEGG enrichment. Furthermore, BHD-IS PPI network, compound-compound target-IS network and pathway of drug-compound target-IS network were constructed by Cytoscape 3.6.0. Finally, AutoDock was used for molecular docking verification.Results：A total of 253 putative targets were obtained from 60 active compounds in BHD. Among them, 62 targets were related to IS. PPI network showed that the top ten key targets were IL6, TNF, VEGFA, and AKT1, etc. The enrichment analysis demonstrated candidate BHD targets were more frequently involved TNF, PI3K-Akt, and NF-kappa B signaling pathway. Network topology analysis showed that Radix Astragali was the main herb in BHD, and the key components were quercetin, beta-Sitosterol, kaempferol, and stigmasterol, etc. The results of molecular docking showed the active components in BHD had a good binding ability with the key targets.Conclusions: This study firstly adopted the methods of network pharmacology and molecular docking to reveal the relationships among herbs in BHD, the putative targets and IS-related pathways.

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“…Network pharmacology is a comprehensive approach based on traditional pharmacology, bioinformatics, chemoinformatics, and network biology. is approach enables researchers to explore regulation of the signaling pathways with multiple targets and optimize the objective of clinical trials [28]. It is widely considered as a supplementary approach to identify components and pharmacological mechanisms of Chinese herbal medicine from a comprehensive perspective [29].…”

Section: Discussionmentioning

confidence: 99%

Uncovering Synergistic Mechanism of Chinese Herbal Medicine in the Treatment of Atrial Fibrillation with Obstructive Sleep Apnea Hypopnea Syndrome by Network Pharmacology

Mao

Zhang

Qiao

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Paroxysmal atrial fibrillation (AF) combined with obstructive sleep apnea hypopnea syndrome (OSAHS) is very common in clinical practice. Traditional Chinese medicine (TCM) rule of regulating the liver based on psycho-cardiology shows satisfactory effectiveness in the treatment of paroxysmal AF combined with OSAHS. However, its underlying pharmacological mechanism has not yet been elucidated. This study applied network pharmacology to identify 94 active components in the six TCM liver-regulating herbs and 182 corresponding targets from several databases and comprehensive literature studies, as well as retrieved AF combined with OSAHS-related targets. Cytoscape software was adopted to construct the component-component target network and component-putative target-AF combined with OSAHS target network. Then, we obtained 38 putative therapeutic targets against AF combined with OSAHS. After the production of a putative therapeutic target interaction network, topological analysis was adopted to determine the core targets of TCM liver-regulating herbs in the treatment of paroxysmal AF combined with OSAHS. For all putative therapeutic targets, biological process analysis and pathway enrichment analysis were utilized to investigate the possible mechanism of TCM liver-regulating herbs in the treatment of paroxysmal AF combined with OSAHS. Mechanistically, it included positive regulation of nitric oxide biosynthetic process, aging, response to hypoxia, TNF signaling pathway, HIF-1 signaling pathway, PI3K-Akt signaling pathway, neuroactive ligand-receptor interaction, and calcium signaling pathway. Especially, six core targets of TCM liver-regulating herbs, namely, TNF, STAT3, AKT1, IL-6, TP53, and INS, were significant in the regulation of the above biological processes and pathways. This study demonstrates the multicomponent, multitarget, and multipathway feature of TCM liver-regulating herbs, provides an extensional foundation for further research, and facilitates the reasonable application of TCM liver-regulating herbs in treating paroxysmal AF combined with OSAHS.

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Network Pharmacology in Research of Chinese Medicine Formula: Methodology, Application and Prospective

Cited by 510 publications

References 94 publications

Investigation of cardiovascular protective effect of Shenmai injection by network pharmacology and pharmacological evaluation

Investigation of cardiovascular protective effect of Shenmai injection by network pharmacology and pharmacological evaluation

Network pharmacology and molecular docking analysis on molecular targets and mechanisms of Buyang Huanwu Decoction in the treatment of Ischemic Stroke

Uncovering Synergistic Mechanism of Chinese Herbal Medicine in the Treatment of Atrial Fibrillation with Obstructive Sleep Apnea Hypopnea Syndrome by Network Pharmacology

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