Network polymers incorporating lipid-bilayer disrupting polymers: towards antiviral functionality

Burridge, Kevin M.; Rahman, Monica Sharfin; Watuthanthrige, Nethmi De Alwis; Gordon, Emma A; Shah, Muhammad Zeeshan; Chandrarathne, Bhagya Madhushani; Lorigan, Gary A.; Page, Richard C.; Konkolewicz, Dominik

doi:10.1039/d2py00602b

Cited by 2 publications

(8 citation statements)

References 65 publications

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“…The materials with shorter chain lengths and lower crosslink density exhibited a lower T g value. However, all the T g values of the unfunctionalized materials were comparable to the literature values 14 . Most importantly, functionalization with 2% peptide had a negligible effect on the T g value of the materials.…”

Section: Resultssupporting

confidence: 84%

“…This is likely due to the higher density of thiols more efficiently binding the SBP1 peptide giving a more substantial impact on spike protein capture. Further, previous research from our group showed significant reproducibility in spike protein capture by these network systems 14 …”

Section: Resultsmentioning

confidence: 78%

“…Considering the effect of copolymer microstructure, previous research from our group demonstrated the scanning electron microscopy images of covalent crosslinked network of HEA system in different chain length and crosslinker densities. It was observed that these materials exhibited almost no significant effect of copolymer microstructure 14 …”

Section: Resultsmentioning

confidence: 99%

“…Hence, developing a surface with antiviral properties by immobilizing inhibitors to capture the viruses with subsequent inactivation could be used to control the spread of SARS‐CoV‐2. Earlier work in our group showed that appropriately functionalized polymers can disrupt the lipids typical of enveloped proteins and had some possibility of capturing the spike protein fragments of SARS‐CoV‐2 14,15 …”

Section: Introductionmentioning

confidence: 99%

“…Earlier work in our group showed that appropriately functionalized polymers can disrupt the lipids typical of enveloped proteins and had some possibility of capturing the spike protein fragments of SARS-CoV-2. 14,15 Recent studies have established that SARS-CoV-2 coronavirus uses its spike protein to bind with the angiotensin-converting enzyme 2 (ACE2) receptors on the human cell surface to invade humans. 7,8,16,17,18 Inhibitors based on the key amino acid residues involved in this SARS-CoV-2/ACE2 protein-protein interaction could be effective in preventing viral entry into human cells.…”

Section: Introductionmentioning

confidence: 99%

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SARS‐CoV‐2 spike protein capture by peptide functionalized networks

Rahman

Rajawasam

Watuthanthrige

et al. 2022

Journal of Polymer Science

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Coronavirus disease 2019 (COVID-19) has significantly impacted human health, the global economy, and society. Viruses residing on common surfaces represent a potential source of contamination for the general population. Spike binding peptide 1, SBP1 is a 23 amino acid peptide, which has micromolar binding affinity (1.3 μM) towards the spike protein receptor-binding domain. We hypothesize that if we can covalently immobilize this SBP1 peptide in a covalent crosslinked network system, we can develop a surface that would preferentially bind spike protein and, therefore, which could limit viral spread. A series of covalently crosslinked networks of hydroxy ethyl acrylate (HEA) with different primary chain lengths and crosslinker density was prepared. Later, this network system was functionalized using 2% SBP1 peptide. Our study found that with a shorter chain length and lower crosslinker density, the HEA network system alone could capture almost 80% of the spike protein. We reported that the efficiency could be enhanced almost by 17% with higher crosslinker density.

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Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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SARS‐CoV‐2 spike protein capture by peptide functionalized networks

Rahman

Rajawasam

Watuthanthrige

et al. 2022

Journal of Polymer Science

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Vinyl Ether Maleic Acid Polymers: Tunable Polymers for Self-Assembled Lipid Nanodiscs and Environments for Membrane Proteins

Shah,

Rotich,

Okorafor

et al. 2024

Biomacromolecules

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Native lipid bilayer mimetics, including those that use amphiphilic polymers, are important for the effective study of membrane-bound peptides and proteins. Copolymers of vinyl ether monomers and maleic anhydride were developed with controlled molecular weights and hydrophobicity through reversible addition−fragmentation chain-transfer polymerization. After polymerization, the maleic anhydride units can be hydrolyzed, giving dicarboxylates. The vinyl ether and maleic anhydride copolymerized in a close to alternating manner, giving essentially alternating hydrophilic maleic acid units and hydrophobic vinyl ether units along the backbone after hydrolysis. The vinyl ether monomers and maleic acid polymers self-assembled with lipids, giving vinyl ether maleic acid lipid particles (VEMALPs) with tunable sizes controlled by either the vinyl ether hydrophobicity or the polymer molecular weight. These VEMALPs were able to support membrane-bound proteins and peptides, creating a new class of lipid bilayer mimetics.

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Network polymers incorporating lipid-bilayer disrupting polymers: towards antiviral functionality

Abstract: Designing a surface that can disinfect itself can reduce labor-intensive cleanings and harmful waste, and mitigate spread of surface borne diseases. Additionally, since COVID-19 being an airborne pathogen, surface modification...

Cited by 2 publications

References 65 publications

SARS‐CoV‐2 spike protein capture by peptide functionalized networks

SARS‐CoV‐2 spike protein capture by peptide functionalized networks

Vinyl Ether Maleic Acid Polymers: Tunable Polymers for Self-Assembled Lipid Nanodiscs and Environments for Membrane Proteins

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