Neumonía por Mycoplasma pneumoniae: una rara causa de distrés respiratorio del adulto

Chiner, E.; Signes-Costa, Jaime; Andreu, Ada Luz; Andreu, Lurdes Matas

doi:10.4321/s0212-71992003001100011

Cited by 2 publications

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“…Mycoplasma genitalium is a human pathogen causing Chlamydia-negative non-gonococcal urethritis and it is phylogenetically related to M. pneumoniae, the causative agent of walking pneumonia and other extra-pulmonary pathologies in humans (Chiner et al, 2003;Jensen, 2004). With only 525 ORFs, M. genitalium has the smallest genome of any organism that can be grown in pure or axenic culture, which makes it an appealing model of a minimal cell.…”

Section: Introductionmentioning

confidence: 99%

A new promoterless reporter vector reveals antisense transcription in Mycoplasma genitalium

et al. 2007

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The mechanisms that promote and regulate transcription in mycoplasmas are poorly understood. Here, a promoter-probe vector based on the pMTnTetM438 minitransposon and containing a promoterless lacZ reporter gene was constructed to analyse Mycoplasma genitalium transcription in vivo. Recovered transposon insertions were in monocopy, with 16 % expressing enough b-galactosidase (b-Gal) to yield colonies exhibiting a detectable blue colour. A sample of 52 blue colonies was propagated and selected for further analyses. The b-Gal activity of the corresponding cultures was measured to quantify, in a reproducible way, the transcription levels of the interrupted ORFs. Several insertions were found in sense with the interrupted ORF, but surprisingly there was also a number of insertions in non-coding regions, many of them in repetitive DNA regions known as MgPa islands. Moreover, 30 % of the analysed transposon insertions had the lacZ gene in the opposite orientation to the coding frame, suggesting the existence of antisense transcripts that may be involved in the control of gene expression in M. genitalium.

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Section: Introductionmentioning

confidence: 99%

A new promoterless reporter vector reveals antisense transcription in Mycoplasma genitalium

et al. 2007

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Comparative “-omics” in Mycoplasma pneumoniae Clinical Isolates Reveals Key Virulence Factors

et al. 2015

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The human respiratory tract pathogen M. pneumoniae is one of the best characterized minimal bacterium. Until now, two main groups of clinical isolates of this bacterium have been described (types 1 and 2), differing in the sequence of the P1 adhesin gene. Here, we have sequenced the genomes of 23 clinical isolates of M. pneumoniae. Studying SNPs, non-synonymous mutations, indels and genome rearrangements of these 23 strains and 4 previously sequenced ones, has revealed new subclasses in the two main groups, some of them being associated with the country of isolation. Integrative analysis of in vitro gene essentiality and mutation rates enabled the identification of several putative virulence factors and antigenic proteins; revealing recombination machinery, glycerol metabolism and peroxide production as possible factors in the genetics and physiology of these pathogenic strains. Additionally, the transcriptomes and proteomes of two representative strains, one from each of the two main groups, have been characterized to evaluate the impact of mutations on RNA and proteins levels. This study has revealed that type 2 strains show higher expression levels of CARDS toxin, a protein recently shown to be one of the major factors of inflammation. Thus, we propose that type 2 strains could be more toxigenic than type 1 strains of M. pneumoniae.

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Neumonía por Mycoplasma pneumoniae: una rara causa de distrés respiratorio del adulto

Cited by 2 publications

References 0 publications

A new promoterless reporter vector reveals antisense transcription in Mycoplasma genitalium

A new promoterless reporter vector reveals antisense transcription in Mycoplasma genitalium

Comparative “-omics” in Mycoplasma pneumoniae Clinical Isolates Reveals Key Virulence Factors

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