Neural alterations from lead exposure in zebrafish

Roy, Nicole M.; DeWolf, Sarah; Schutt, Alexius; Wright, Ashia; Steele, LaTina

doi:10.1016/j.ntt.2014.08.008

Cited by 19 publications

(7 citation statements)

References 26 publications

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“…Previous studies in our laboratory do indicate that developing zebrafish exposed to this concentration of Pb (100 ppb) do not have an increase in gross or minor morphological alterations in total larval length, head length or head width (Wirbisky et al ., ). While studies at higher Pb concentrations do report an increase in apoptosis in the brain and structural alterations in the hindbrain (e.g., Roy et al ., ), we have found that the exposure concentration used in this study does not increase apoptosis in the brain or result in alterations in brain length in developing zebrafish (Peterson et al ., ). Furthermore, while findings of the current study do support a developmental origin of adult Pb toxicity, further work is needed to determine the mechanisms altering the innate color preference.…”

Section: Discussionmentioning

confidence: 53%

Directional and color preference in adult zebrafish: Implications in behavioral and learning assays in neurotoxicology studies

Bault

Peterson

Freeman

2015

J of Applied Toxicology

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The zebrafish (Danio rerio) is a useful vertebrate model organism for neurological studies. While a number of behavior and learning assays are recently reported in the literature for zebrafish, many of these assays are still being refined. The initial purpose of this study was to apply a published T-maze assay for adult zebrafish that measures how quickly an organism can discriminate between different color stimuli after receiving reinforcement to measure learning in a study investigating the later life impacts of developmental Pb exposure. The original results were inconclusive as the control group showed a directional and color preference. To assess directional preference further, a three-chambered testing apparatus was constructed and rotated in several directions. The directional preference observed in males was alleviated by rotating the arms pointing west and east. In addition, color preference was investigated using all combinations of five different colors (orange, yellow, green, blue and purple). With directional preference alleviated results showed that both male and female zebrafish preferred colors of shorter wavelengths. An additional experiment tested changes in color preference due to developmental exposure to Pb in adult male zebrafish. Results revealed that Pb-exposed males gained and lost certain color preferences compared to control males and the preference for short wavelengths was decreased. Overall, these results show that consideration and pretesting should be completed before applying behavioral and learning assays involving adult zebrafish to avoid innate preferences and confounding changes in neurotoxicology studies and that developmental Pb exposure alters color preferences in adult male zebrafish.

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Section: Discussionmentioning

confidence: 53%

Directional and color preference in adult zebrafish: Implications in behavioral and learning assays in neurotoxicology studies

Bault

Peterson

Freeman

2015

J of Applied Toxicology

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“…Zebrafish is considered an excellent animal model for investigation of neurodevelopmental disorders (23) and has been previously used to study cerebellar development (24), hindbrain (25) as well as motor neurons (26). The lightly pigmented golden strain and islet1 :GFP transgenic zebrafish were chosen to perform downregulation of rbm7 , exosc8 and exosc3.…”

Section: Genetic Analysismentioning

confidence: 99%

Altered RNA metabolism due to a homozygousRBM7mutation in a patient with spinal motor neuropathy

Giunta

Edvardson

et al. 2016

Hum. Mol. Genet.

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The exosome complex is the most important RNA processing machinery within the cell. Mutations in its subunits EXOSC8 and EXOSC3 cause pontocerebellar hypoplasia, spinal muscular atrophy (SMA) and central nervous system demyelination. We present a patient with SMA-like phenotype carrying a homozygous mutation in RBM7—a subunit of the nuclear exosome targeting (NEXT) complex—which is known to bind and carry specific subtypes of coding and non-coding RNAs to the exosome. The NEXT complex with other protein complexes is responsible for the substrate specificity of the exosome. We performed RNA-sequencing (RNA-seq) analysis on primary fibroblasts of patients with mutations in EXOSC8 and RBM7 and gene knock-down experiments using zebrafish as a model system. RNA-seq analysis identified significantly altered expression of 62 transcripts shared by the two patient cell lines. Knock-down of rbm7, exosc8 and exosc3 in zebrafish showed a common pattern of defects in motor neurons and cerebellum. Our data indicate that impaired RNA metabolism may underlie the clinical phenotype by fine tuning gene expression which is essential for correct neuronal differentiation.

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“…Several studies have demonstrated the neurotoxic effects of lead exposure at early embryonic stages, thereby causing some changes in the development of the nervous system. Zebrafish embryos treated with lead at 12-72 h post-fertilization (hpf) displayed alterations in neural ventricle formation, hindbrain branchiomotor neurons and neural vasculature, and increased apoptosis [22]. Low-level lead exposure during development resulted in embryotoxicity, behavioral disorders, and learning and memory impairments in zebrafish [6].…”

mentioning

confidence: 99%

Chronic Exposure to Low Concentration Lead Chloride-Induced Anxiety and Loss of Aggression and Memory in Zebrafish

Thi

Audira

et al. 2020

IJMS

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Lead and lead-derived compounds have been extensively utilized in industry, and their chronic toxicity towards aquatic animals has not been thoroughly addressed at a behavioral level. In this study, we assessed the risk of exposure to lead at a waterborne environmental concentration in adult zebrafish by behavioral and biochemical analyses. Nine tests, including three-dimension (3D) locomotion, novel tank exploration, mirror biting, predator avoidance, social interaction, shoaling, circadian rhythm locomotor activity, color preference, and a short-term memory test, were performed to assess the behavior of adult zebrafish after the exposure to 50 ppb PbCl 2 for one month. The brain tissues were dissected and subjected to biochemical assays to measure the relative expression of stress biomarkers and neurotransmitters to elucidate the underlying mechanisms for behavioral alterations. The results of the behavioral tests showed that chronic exposure to lead could elevate the stress and anxiety levels characterized by elevated freezing and reduced exploratory behaviors. The chronic exposure to PbCl 2 at a low concentration also induced a sharp reduction of aggressiveness and short-term memory. However, no significant change was found in predator avoidance, social interaction, shoaling, or color preference. The biochemical assays showed elevated cortisol and reduced serotonin and melatonin levels in the brain, thus, altering the behavior of the PbCl 2 -exposed zebrafish. In general, this study determined the potential ecotoxicity of long-term lead exposure in adult zebrafish through multiple behavioral assessments. The significant findings were that even at a low concentration, long-term exposure to lead could impair the memory and cause a decrease in the aggressiveness and exploratory activities of zebrafish, which may reduce their survival fitness.(EC10/NOEC-usually more than 14 days) on fish range from 18 to 1559 µg/L and 44 to 437 µg/L, respectively [3,4].More evidence has shown the detrimental impacts of lead on human health, especially at a neurobehavioral level. The neurotoxicity of chronic exposure to low concentrations of lead can impair cognitive performance in childhood through to adulthood [5][6][7][8][9]. Some studies demonstrated the correlation between the exposure to lead at low concentrations and attention-deficit/hyperactivity disorder (ADHD) in children [10]. The development of the central nervous system can be disrupted when children are exposed to low concentrations of lead for a long period of time (blood lead level below 10 mg/dL) [11]. Therefore, lead toxicity has become a serious issue for human health and environmental protection [6]. According to previous studies, the effect of lead on the nervous system includes the disruption of key molecules during neuronal migration and differentiation, interference with synapse formation mediated by a reduction in neuronal sialic acid production and premature differentiation of glial cells, interference with neurotransmitter release, and disruption of the...

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Neural alterations from lead exposure in zebrafish

Cited by 19 publications

References 26 publications

Directional and color preference in adult zebrafish: Implications in behavioral and learning assays in neurotoxicology studies

Directional and color preference in adult zebrafish: Implications in behavioral and learning assays in neurotoxicology studies

Altered RNA metabolism due to a homozygousRBM7mutation in a patient with spinal motor neuropathy

Chronic Exposure to Low Concentration Lead Chloride-Induced Anxiety and Loss of Aggression and Memory in Zebrafish

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