Neural Coding of Leg Proprioception in Drosophila

Mamiya, Akira; Gurung, Pralaksha; Tuthill, John C.

doi:10.1016/j.neuron.2018.09.009

Cited by 123 publications

(217 citation statements)

References 83 publications

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“…4e) and identify the dorsally-branching clusters as motor neurons (Baek and Mann, 2009;Brierley et al, 2012) and the ventrallybranching clusters as sensory neurons (Tsubouchi et al, 2017). In some cases, we were also able to associate the neurons with specific sensory organs, such as the campaniform sensilla and femoral chordatonal organs (Mamiya et al, 2018). We observed that axons are spatially organized in the nerve, such that axons from neurons from the same morphological cluster tend to also physically cluster together within the nerve (Fig.…”

Section: Reconstruction Of Individual Neuron Morphologiesmentioning

confidence: 70%

“…The indicated motor neuron is the same neuron as in (h) (g) Cross-section through the femur. Asterisk denotes the femoral chordatonal organ, a proprioceptive sensory structure (Mamiya et al, 2018). (h) Detailed view of nerve within the femur, including a motor axon branching off to innervate a muscle (arrow).…”

Section: Millimeter-scale Xnh Imaging Of a Drosophila Leg At Single-nmentioning

confidence: 99%

“…(Baek and Mann, 2009;Brierley et al, 2012) and those arborizing ventrally as sensory neurons (Tsubouchi et al, 2017). Two major subtypes of leg sensory neurons, campaniform sensilla and chordotonal neurons, were identified based on arborization pattern in the VNC (Mamiya et al, 2018).…”

Section: Automated Segmentation Of Neuronal Morphologies Using Convolmentioning

confidence: 99%

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Dense neuronal reconstruction through X-ray holographic nano-tomography

Pacureanu

Maniates-Selvin

Kuan

et al. 2019

Preprint

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Elucidating the structure of neuronal networks provides a foundation for understanding how the nervous system processes information to generate behavior. Despite technological breakthroughs in visible light and electron microscopy, imaging dense nanometer-scale neuronal structures over millimeter-scale tissue volumes remains a challenge. Here, we demonstrate that X-ray holographic nano-tomography is capable of imaging large tissue volumes with sufficient resolution to disentangle dense neuronal circuitry in Drosophila melanogaster and mammalian central and peripheral nervous tissue. Furthermore, we show that automatic segmentation using convolutional neural networks enables rapid extraction of neuronal morphologies from these volumetric datasets. The technique we present allows rapid data collection and analysis of multiple specimens, and can be used correlatively with light microscopy and electron microscopy on the same samples. Thus, X-ray holographic nano-tomography provides a new avenue for discoveries in neuroscience and life sciences in general.

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Section: Reconstruction Of Individual Neuron Morphologiesmentioning

confidence: 70%

Section: Millimeter-scale Xnh Imaging Of a Drosophila Leg At Single-nmentioning

confidence: 99%

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Dense neuronal reconstruction through X-ray holographic nano-tomography

Pacureanu

Maniates-Selvin

Kuan

et al. 2019

Preprint

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“…Some hemilineages have dendritic processes restricted to specific sensory domains suggesting involvement in the processing of specific sensory modalities. For example, hemilineage 10B has dendrites tightly restricted to mVAC and intermediate neuropil indicative of being involved in processing of sensory information from the “club” type sensory afferents from the femoral chordotonal organ (FCO; Mamiya, Gunung, & Tuthill, ) and from campaniform sensilla on the legs. The 10B neurons also have intersegmental projections that overlap with the dendrites of their segmental homologs suggesting a role in coordinating sensory inputs between different legs.…”

Section: Discussionmentioning

confidence: 99%

Developmental organization of central neurons in the adult Drosophila ventral nervous system

Shepherd

Sahota

Court

et al. 2019

J of Comparative Neurology

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We have used MARCM to reveal the adult morphology of the post embryonically produced neurons in the thoracic neuromeres of the Drosophila VNS. The work builds on previous studies of the origins of the adult VNS neurons to describe the clonal organization of the adult VNS. We present data for 58 of 66 postembryonic thoracic lineages, excluding the motor neuron producing lineages (15 and 24) which have been described elsewhere. MARCM labels entire lineages but where both A and B hemilineages survive (e.g., lineages 19, 12, 13, 6, 1, 3, 8, and 11), the two hemilineages can be discriminated and we have described each hemilineage separately. Hemilineage morphology is described in relation to the known functional domains of the VNS neuropil and based on the anatomy we are able to assign broad functional roles for each hemilineage. The data show that in a thoracic hemineuromere, 16 hemilineages are primarily involved in controlling leg movements and walking, 9 are involved in the control of wing movements, and 10 interface between both leg and wing control. The data provide a baseline of understanding of the functional organization of the adult Drosophila VNS. By understanding the morphological organization of these neurons, we can begin to define and test the rules by which neuronal circuits are assembled during development and understand the functional logic and evolution of neuronal networks.

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“…Considering the broad expression of serotonergic receptors in sensory organs, it is 503 interesting that one of the behavioral roles of serotonin we identified is its ability to mediate the 504 response to vibration. Vibration is sensed by the chordotonal organ, and our expression 505 analysis reveals that serotonin receptors are expressed to different extents in chordotonal 506 neurons [100][101][102]. Together, these observations suggest that modulation of sensory 507 information as it is entering the VNC plays a key role in how serotonin modulates the response 508 to a vibration stimulus.…”

mentioning

confidence: 83%

Serotonergic modulation of walking inDrosophila

Howard

Chen

Tabachnik

et al. 2019

Preprint

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16To navigate complex environments, animals must generate highly robust, yet flexible, locomotor 17 behaviors. For example, walking speed must be tailored to the needs of a particular 18 environment: Not only must animals choose the correct speed and gait, they must also rapidly 19 adapt to changing conditions, and respond to sudden and surprising new stimuli. 20Neuromodulators, particularly the small biogenic amine neurotransmitters, allow motor circuits 21 to rapidly alter their output by changing their functional connectivity. Here we show that the 22 serotonergic system in the vinegar fly, Drosophila melanogaster, can modulate walking speed in 23 a variety of contexts and in response to sudden changes in the environment. These multifaceted 24 roles of serotonin in locomotion are differentially mediated by a family of serotonergic receptors 25 with distinct activities and expression patterns. 27walk forwards, backwards, and upside down, navigate complex terrains, and rapidly recover 31 after injury [1][2][3][4][5][6][7][8][9]. To achieve this wide range of behaviors, insects regulate their global walking 32 speed and kinematic parameters, allowing them to modify stereotyped gaits as needed [3,5-33 7,9,10]. Because overlapping sets of motor neurons and muscles are recruited for all of these 34 behaviors, animals must be able to rapidly modulate the circuit dynamics that control locomotor 35 parameters [11-13] (reviewed in [14]). 36As with limbed vertebrates, most insects use multi-jointed legs to walk [8,10,[15][16][17]. Locomotor 37 circuits that orchestrate these complex gaits are located in the ventral nerve cord (VNC), a 38 functional analogue of the vertebrate spinal cord that includes three pairs of thoracic 39 neuromeres (T1, T2, and T3) that coordinate the movements of the three pairs of thoracic legs 40 [1][2][3][4][5][6][7][8][9][18][19][20]. The insect VNC receives descending commands from the brain and sends motor 41 output instructions via motor neurons to peripheral musculature [3,[5][6][7]9,10,19]. Leg motor 42 neuron dendrites innervate the leg neuropils within the VNC and their axons exit the VNC to 43 synapse onto muscles in the appendages [11][12][13]21,22]. Sensory neurons, which convey 44 proprioceptive and tactile information, project axons from the appendages to the VNC by these 45 same fiber tracts, where they arborize in the leg neuropils [14,23,24] ( Figure 1A). Notably, the 46 VNC is capable of executing coordinated leg motor behaviors, such as walking and grooming, 48 drive the coordinated flexion and extension of each leg joint and, therefore, walking gaits 49 [8,10,17,20,26]. 50Numerous studies have shown that sensory input from the legs are required for robust 51 and stereotyped locomotor patterns, regulating both the timing and magnitude of locomotor 52 activity and also facilitating coordination between legs [6,8,10,12,[27][28][29]. However, sensory 53 feedback cannot be the only means for tuning locomotion: mutation of proprioceptive receptors 54 or even deafferenting limbs d...

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Neural Coding of Leg Proprioception in Drosophila

Cited by 123 publications

References 83 publications

Dense neuronal reconstruction through X-ray holographic nano-tomography

Dense neuronal reconstruction through X-ray holographic nano-tomography

Developmental organization of central neurons in the adult Drosophila ventral nervous system

Serotonergic modulation of walking inDrosophila

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