Neural differentiation of novel multipotent progenitor cells from cryopreserved human umbilical cord blood

Lee, Myoung Woo; Moon, Young Joon; Yang, Mal Sook; Kim, Sun Kyung; Jang, In Keun; Eom, Young-Woo; Park, Joon Seong; Kim, Hugh C.; Song, Kye Yong; Park, Soon Cheol; Lim, Hwan Sub; Kim, Young Jin

doi:10.1016/j.bbrc.2007.04.181

Cited by 36 publications

(28 citation statements)

References 24 publications

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“…The multipotentiality of these cells to produce the main cell types of nervous tissue has been recently reported. 25,57 These cells have also been used in experimental SCI models and were shown to improve functional recovery due to neurotrophic function 70 or increased myelination. 25 However, further work must be done to fully characterize the extent of functional recovery induced by umbilical cord blood cells and to further delineate their mechanisms of action.…”

Section: Fetal Stem Cellsmentioning

confidence: 99%

Current status of experimental cell replacement approaches to spinal cord injury

Eftekharpour

Karimi-Abdolrezaee

Fehlings

2008

FOC

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✓ Despite advances in medical and surgical care, the current clinical therapies for spinal cord injury (SCI) are largely ineffective. During the last 2 decades, the search for new therapies has been revolutionized by the discovery of stem cells, which has inspired scientists and clinicians to search for a stem cell–based reparative approaches to many diseases, including neurotrauma. In the present study, the authors briefly summarize current knowledge related to the pathophysiology of SCI, including the concepts of primary and secondary injury and the importance of posttraumatic demyelination. Key inhibitory obstacles that impede axonal regeneration include the glial scar and a number of myelin inhibitory molecules including Nogo. Recent advancements in cell replacement therapy as a therapeutic strategy for SCI are summarized. The strategies include the use of pluripotent human stem cells, embryonic stem cells, and a number of adult-derived stem and progenitor cells such as mesenchymal stem cells, Schwann cells, olfactory ensheathing cells, and adult-derived neural precursor cells. Although current strategies to repair the subacutely injured cord appear promising, many obstacles continue to render the treatment of chronic injuries challenging. Nonetheless, the future for stem cell–based reparative strategies for treating SCI appears bright.

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Section: Fetal Stem Cellsmentioning

confidence: 99%

Current status of experimental cell replacement approaches to spinal cord injury

Eftekharpour

Karimi-Abdolrezaee

Fehlings

2008

FOC

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“…These include umbilical hematopoietic stem cells, bone marrow stem cells, neural stem cells, embryonic stem cells, skin precursor cells and glial restricted precursor cells. 30,[32][33][34][35][36][37][38] There are a multitude of strategies utilized to synthesize astrocytes under user-defined in-vitro conditions. These include treatments of the stem/ progenitor/precursor cell sources with retinoic acid 39 , growing cells on a layer of endothelial cells 40 or astrocytes 41 , growth in the presence of cytokines such as Epidermal growth factor (EGF) 42 , Ciliary neurotrophic factor (CNTF) 43 , Activin 44 , Fibroblast growth factors (FGFs) 45 and Bone Morphogenetic proteins.…”

Section: Sources Of Synthetic Astrocytesmentioning

confidence: 99%

Stem cells and models of astrocytomas

Kamnasaran

2009

CIM

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Clin Invest Med 2009; 32 (2): E166-E179. AbstractPurpose: To provide a critical assessment of current stemcell based pre-clinical models of astrocytomas (gliomas). Methods: Data were archived from MEDLINE using Boolean formatted keyword queries. Top articles were selected for critical analyses depending on the qualitative assessment of the citation index, novelty of the findings, reputation of the research group and relevance to stem-cell based pre-clinical models of astrocytomas. Results: The emergence of stem-cell based pre-clinical models of gliomas offers advantages for cellular transformation studies over other current in-vitro cell cultured based models. Cells utilized in these stem-cell based preclinical models are easier to transform, with the induced tumours demonstrating very high molecular and pathological recapitulations of astrocytomas that are derived from humans. These stem-cell based models fall into two categories. In the first, synthetic astrocytes can be differentiated from various stem cell sources such as the nervous system and embryos, and utilized in elegant forward genetic strategies to develop novel astrocytoma pre-clinical models. The second category represents a cancer stem cell pre-clinical model. In this model, glioma stem cells exhibit very high pathological recapitulations of the human tumours, and can be very informative to comprehend the basis of radio-chemoresistance among patients. Conclusion: The quest to develop robust pre-clinical models of astrocytomas is on an ongoing basis. The models are of clinical importance for the discovery of effective treatment modalities that can considerably improve the health of patients with this deadly disease.

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“…Initial studies by Ha et al [3] have shown that neural markers are expressed in cultured human UCB monocytes. In vitro studies have further demonstrated the neural differentiation potential of UCB-derived MSCs [7,13,18]. Some in vivo experiments have shown functional improvements in corresponding animal model systems following UCB-derived stem cell transplantation [4,5,17,19].…”

mentioning

confidence: 99%

“…Since these cells can not only differentiate into cells of connective tissue and neural lineages [3,7,13,18], but also possess many advantages involving immaturation, less immunogenicity, without ethical problems and without harm to the mother or infant. Initial studies by Ha et al [3] have shown that neural markers are expressed in cultured human UCB monocytes.…”

mentioning

confidence: 99%

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Generation of Neuronal-Like Cells from Umbilical Cord Blood-Derived Mesenchymal Stem Cells of a RFP-Transgenic Cloned Cat

Jin

Yin

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. Umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) are multipotent adult stem cells, which can differentiation into cells of connective tissue and neural lineages. This study investigated the potential for neuronal differentiation of red fluorescent protein (RFP)-transgenic cat UCB-derived MSCs. The cells were cultured in pre-induction medium for 24 hr and in neuronalinduction medium for 72 hr. Immunofluorescent staining showed that 6.85% of the total cells were β III-tubulin-positive, 3.37% were neurofilament light (NF-L)-positive and 7.04% were neurofilament medium (NF-M)-positive. A β III-tubulin band was detected by western blot analysis. Our results demonstrate that RFP-transgenic UCB-derived MSCs can be differentiated into neuronal cells in vitro. Thus, RFP-transgenic MSCs could provide alternative tracing material for stem cell transplantation.

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Neural differentiation of novel multipotent progenitor cells from cryopreserved human umbilical cord blood

Cited by 36 publications

References 24 publications

Current status of experimental cell replacement approaches to spinal cord injury

Current status of experimental cell replacement approaches to spinal cord injury

Stem cells and models of astrocytomas

Generation of Neuronal-Like Cells from Umbilical Cord Blood-Derived Mesenchymal Stem Cells of a RFP-Transgenic Cloned Cat

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