Neural Endophenotype Assessment in Zebrafish Larvae Using Optomotor and ZebraBox Locomotion Assessment

Xie, Jiaheng; Goodbourn, Patrick; Sztal, Tamar; Jusuf, Patricia R.

doi:10.1007/978-1-0716-3585-8_17

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…In contrast, the optomotor response (OMR) assay evaluates the behavioral locomotor response of animals stimulated by moving black and white stripe patterns. In those assays, visual functions such as spatial resolution or acuity and temporal resolution are evaluated by adjusting stripe width, the speed of stripe movement and the intensity of light exposure [ 16 , 22 – 26 ]. OKR and OMR assays have been used in studies to explore the genetic and molecular mechanisms underlying retinal development [ 23 ], retinal degenerative disease [ 27 ], neural activities [ 28 , 29 ], as well as animal behavior [ 30 ] in various models.…”

Section: Introductionmentioning

confidence: 99%

Characterizing medaka visual features using a high-throughput optomotor response assay

Suzuki,

Woo,

Thumberger

et al. 2024

PLoS ONE

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Medaka fish (Oryzias latipes) is a powerful model to study genetics underlying the developmental and functional traits of the vertebrate visual system. We established a simple and high-throughput optomotor response (OMR) assay utilizing medaka larvae to study visual functions including visual acuity and contrast sensitivity. Our assay presents multiple adjustable stripes in motion to individual fish in a linear arena. For that the OMR assay employs a tablet display and the Fish Stripes software to adjust speed, width, color, and contrast of the stripes. Our results demonstrated that optomotor responses were robustly induced by black and white stripes presented from below in the linear-pool-arena. We detected robust strain specific differences in the OMR when comparing long established medaka inbred strains. We observed an interesting training effect upon the initial exposure of larvae to thick stripes, which allowed them to better respond to narrower stripes. The OMR setup and protocol presented here provide an efficient tool for quantitative phenotype mapping, addressing visual acuity, trainability of cortical neurons, color sensitivity, locomotor response, retinal regeneration and others. Our open-source setup presented here provides a crucial prerequisite for ultimately addressing the genetic basis of those processes.

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Section: Introductionmentioning

confidence: 99%

Characterizing medaka visual features using a high-throughput optomotor response assay

Suzuki,

Woo,

Thumberger

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

show abstract

Characterizing medaka visual features using a high-throughput optomotor response assay

Suzuki,

Woo,

Thumberger

et al. 2024

Preprint

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Medaka fish (Oryzias latipes) is a powerful model to study genetics underlying the developmental and functional traits of the vertebrate visual system. We established a simple and high-throughput optomotor response (OMR) assay utilizing medaka larvae to study visual functions including visual acuity and contrast sensitivity. Our assay presents multiple adjustable stripes in motion to individual fish in a linear arena. For that the OMR assay employs a tablet display and theFish Stripessoftware to adjust speed, width, color, and contrast of the stripes. Our results demonstrated that optomotor responses were robustly induced by black and white stripes presented from below in the linear-pool-arena. We detected robust strain specific differences in the OMR when comparing long established medaka inbred strains. We observed an interesting training effect upon the initial exposure of larvae to thick stripes, which allowed them to better respond to narrower stripes. The OMR setup and protocol presented here provide an efficient tool for quantitative phenotype mapping, addressing visual acuity, trainability of cortical neurons, locomotor response, retinal regeneration and others. Our open-source setup presented here provides a crucial prerequisite for ultimately addressing the genetic basis of those processes.

show abstract

Neural Endophenotype Assessment in Zebrafish Larvae Using Optomotor and ZebraBox Locomotion Assessment

Cited by 2 publications

References 14 publications

Characterizing medaka visual features using a high-throughput optomotor response assay

Characterizing medaka visual features using a high-throughput optomotor response assay

Characterizing medaka visual features using a high-throughput optomotor response assay

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