Neural Fate of Mesenchymal Stem Cells and Neural Crest Stem Cells: Which Ways to Get Neurons for Cell Therapy Purpose?

Neirinckx, Virginie; Coste, Cécile; Rogister, Bernard; Wislet, Sabine

doi:10.5772/53260

Cited by 7 publications

(8 citation statements)

References 128 publications

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“…In the case of neurodegeneration, the rate of cell loss exceeds that of generation of new neurons (Venugopal et al, 2017 ). With the balance of damage to regeneration unable to be met by the brain, obtaining neural cells from exogenous sources able to augment or direct repair is an important potential therapeutic tool (Neirinckx et al, 2013 ).…”

Section: Stem Cell Neurogenesismentioning

confidence: 99%

“…Harnessing HSPG and growth factor interactions has also resulted in various forms of HS mimetics and synthetic glycopolymers that mimic natural occurring HSPGs and their function during stem cell neural differentiation. These strategies have produced synthetic glycopolymers with even higher efficacy than heparin (Figure 3 ; Neirinckx et al, 2013 ). In other studies, Liu et al ( 2017 ) devised a phospholipid-anchored GAG-mimicking polymer, termed “lipo-pSGF,” found to promote neural differentiation, likely via the FGF2-Erk1/2 pathway, in mESC cultures and have compared its efficacy to non-anchored pSGF and heparin treated cultures.…”

Section: Approaches For Exploiting Hspgs As Therapeutic Targetsmentioning

confidence: 99%

“…Effective regeneration of brain tissue requires damaged cells be replenished as well as their integration with existing cells to form new functional neural networks (van Velthoven et al, 2009 ). Injuries of the central nervous system (CNS) present therapeutic challenges as current treatments are unable to achieve complete functional recovery, even with symptomatic improvements (Neirinckx et al, 2013 ; Srijaya et al, 2014 ). To circumvent these limitations, a better understanding of the factors that control stem cell neural lineage specification is needed.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Exploiting Heparan Sulfate Proteoglycans in Human Neurogenesis—Controlling Lineage Specification and Fate

Griffiths

Haupt

2017

Front. Integr. Neurosci.

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Unspecialized, self-renewing stem cells have extraordinary application to regenerative medicine due to their multilineage differentiation potential. Stem cell therapies through replenishing damaged or lost cells in the injured area is an attractive treatment of brain trauma and neurodegenerative neurological disorders. Several stem cell types have neurogenic potential including neural stem cells (NSCs), embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and mesenchymal stem cells (MSCs). Currently, effective use of these cells is limited by our lack of understanding and ability to direct lineage commitment and differentiation of neural lineages. Heparan sulfate proteoglycans (HSPGs) are ubiquitous proteins within the stem cell microenvironment or niche and are found localized on the cell surface and in the extracellular matrix (ECM), where they interact with numerous signaling molecules. The glycosaminoglycan (GAG) chains carried by HSPGs are heterogeneous carbohydrates comprised of repeating disaccharides with specific sulfation patterns that govern ligand interactions to numerous factors including the fibroblast growth factors (FGFs) and wingless-type MMTV integration site family (Wnts). As such, HSPGs are plausible targets for guiding and controlling neural stem cell lineage fate. In this review, we provide an overview of HSPG family members syndecans and glypicans, and perlecan and their role in neurogenesis. We summarize the structural changes and subsequent functional implications of heparan sulfate as cells undergo neural lineage differentiation as well as outline the role of HSPG core protein expression throughout mammalian neural development and their function as cell receptors and co-receptors. Finally, we highlight suitable biomimetic approaches for exploiting the role of HSPGs in mammalian neurogenesis to control and tailor cell differentiation into specific lineages. An improved ability to control stem cell specific neural lineage fate and produce abundant cells of lineage specificity will further advance stem cell therapy for the development of improved repair of neurological disorders. We propose a deeper understanding of HSPG-mediated neurogenesis can potentially provide novel therapeutic targets of neurogenesis.

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Section: Stem Cell Neurogenesismentioning

confidence: 99%

Section: Approaches For Exploiting Hspgs As Therapeutic Targetsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Exploiting Heparan Sulfate Proteoglycans in Human Neurogenesis—Controlling Lineage Specification and Fate

Griffiths

Haupt

2017

Front. Integr. Neurosci.

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“…This is generally termed as spontaneous activity and in neurons indicates that the neuronal network is developing and besides it is important for cellular specificity [20]. Since human MSCs are able to transdifferentiate into neurons following several ways, of the utilization of hMSC derived neurons within a clinical aspect, functionality has become important in neuronal differentiation studies [21][22][23]. Only few studies have shown that human mesenchymal stem cell (hMSC)-derived neurons are functionally active upon chemical or electrical excitation [24].…”

Section: Introductionmentioning

confidence: 99%

Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity

et al. 2020

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Mesenchymal stem cells have the ability to transdifferentiate into neurons and therefore one of the potential adult stem cell source for neuronal tissue regeneration applications and understanding neurodevelopmental processes. In many studies on human mesenchymal stem cell (hMSC) derived neurons, success in neuronal differentiation was limited to neuronal protein expressions which is not statisfactory in terms of neuronal activity. Established neuronal networks seen in culture have to be investigated in terms of synaptic signal transmission ability to develop a culture model for human neurons and further studying the mechanism of neuronal differentiation and neurological pathologies. Accordingly, in this study, we analysed the functionality of bone marrow hMSCs differentiated into neurons by a single step cytokine-based induction protocol. Neurons from both primary hMSCs and hMSC cell line displayed spontaneous activity (�75%) as demonstrated by Ca ++ imaging. Furthermore, when electrically stimulated, hMSC derived neurons (hMd-Neurons) matched the response of a typical neuron in the process of maturation. Our results reveal that a combination of neuronal inducers enhance differentiation capacity of bone marrow hMSCs into high yielding functional neurons with spontaneous activity and mature into electrophysiologically active state. Conceptually, we suggest these functional hMd-Neurons to be used as a tool for disease modelling of neuropathologies and neuronal differentiation studies.

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“…In addition to their self-renewal ability, immunomodulatory properties and plasticity, chorion derived MSCs (C-MSCs) are neither teratogenic nor liable of ethical limitations [1,2]. However, as compared to bone marrow MSCs (BM-MSCs) [3] and progenitor cells from other tissues, C-MSCs have been largely under-investigated for neural regenerative therapies.…”

Section: Introductionmentioning

confidence: 99%

cAMP Response Element-Binding Protein Controls the Appearance of Neuron-Like Traits in Chorion Mesenchymal Cells

Innamorati

Ridolfi

Steccanella

et al. 2022

Front. Biosci. (Landmark Ed)

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Background: Mesenchymal stromal cells (MSC) from bone marrow have been reported to undergo the initial phases of neural differentiation in response to an increase of intracellular cAMP. We investigated the possibility that a similar effect applies to chorion-derived MSC. Methods: The intracellular concentration of cAMP was increased either by forskolin, to promote its synthesis, or by inhibitors of its degradation. The consequent reduction in the expression of mesenchymal markers was associated with the appearance of neuronlike morphology in a subset of cells. The effect was measured and characterized using biomarkers and an inhibitor of cAMP response element-binding protein (CREB). Results: The dramatic morphological change induced by all the treatments that promoted intracellular cAMP was transient and peaked on the third day. After that, cells returned to the typical fibroblast-like appearance within 24 hours. The distinctive morphology was associated to the expression of neuregulin 1, doublecortin, neuron-specific class III β-tubulin, and required cAMP response element-binding protein activity. Basic-fibroblast growth factor (b-FGF) treatment increased both the timeframe and number of cells undergoing the morphological change induced by the effect of forskolin. As opposite, arginine-vasopressin (AVP) and sphingosine-1-phosphate (S1P) reduced it. Conclusions: We conclude that cAMP and the ensuing CREB activation trigger a preliminary step towards neuronal differentiation of chorion-derived MSC. However, likewise other MSC, the stimulus is not sufficient to promote stable differentiation.

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Neural Fate of Mesenchymal Stem Cells and Neural Crest Stem Cells: Which Ways to Get Neurons for Cell Therapy Purpose?

Cited by 7 publications

References 128 publications

Exploiting Heparan Sulfate Proteoglycans in Human Neurogenesis—Controlling Lineage Specification and Fate

Exploiting Heparan Sulfate Proteoglycans in Human Neurogenesis—Controlling Lineage Specification and Fate

Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity

cAMP Response Element-Binding Protein Controls the Appearance of Neuron-Like Traits in Chorion Mesenchymal Cells

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