Neural Stem Cell-Conditioned Medium Suppresses Inflammation and Promotes Spinal Cord Injury Recovery

Zhang, Cheng; Bosco, Dale B.; Lee, Sun; Chen, Xiaoming; Xu, Yunsheng; Tai, Wenjiao; Didier, Ruth; Li, Jinhua; Fan, Jianqing; He, Xijing; Ren, Yi

doi:10.3727/096368916x693473

Cited by 56 publications

(52 citation statements)

References 55 publications

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“…Only 28 articles reported sufficient data to perform a statistical test, including subgroup evaluation ( Fig. 1 ) [ 2 3 4 5 6 7 14 15 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 ]. Besides effect size preparation from these 28 studies, other useful data such as the most popular model, the location of injuries and types of stem cell also extracted from the articles and they are shown in Figs.…”

Section: Resultsmentioning

confidence: 99%

Stem cell transplantation and functional recovery after spinal cord injury: a systematic review and meta-analysis

et al. 2018

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Spinal cord injury is a significant cause of motor dysfunctions. There is no definite cure for it, and most of the therapeutic modalities are only symptomatic treatment. In this systematic review and meta-analysis, the effectiveness of stem cell therapy in the treatment of the spinal cord injuries in animal models was studied and evaluated. A systematic search through medical databases by using appropriate keywords was conducted. The relevant reports were reviewed in order to find out cases in which inclusion and exclusion criteria had been fulfilled. Finally, 89 articles have been considered, from which 28 had sufficient data for performing statistical analyses. The findings showed a significant improvement in motor functions after cell therapy. The outcome was strongly related to the number of transplanted cells, site of injury, chronicity of the injury, type of the damage, and the induction of immune-suppression. According to our data, improvements in functional recovery after stem cell therapy in the treatment of spinal cord injury in animal models was noticeable, but its outcome is strongly related to the site of injury, number of transplanted cells, and type of transplanted cells.

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Section: Resultsmentioning

confidence: 99%

Stem cell transplantation and functional recovery after spinal cord injury: a systematic review and meta-analysis

et al. 2018

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“…In addition, the micro-environmental cytotoxicity caused by acute SCI presents considerable obstacles against cellular engraftment [22]. Furthermore, some treatments come with important concerns, including painful responses to nonpainful stimuli after neural stem cell transplantation, ethical concerns, or the formation of teratomas after embryonic stem cell therapy [8]. Hosseini et al [12] reported that BMSCs can differentiate into neural stem cells and neurons.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that less than 1% of MSCs survive for more than 1 week after systemic administration [16]. Loss of differentiation potential, initiation of the host immune response, difficulties in delivery, and poor engraftment of transplanted cells are the major limitations of cell-based therapies [8,24]. Since the main effects of MSCs are likely mediated by paracrine mechanisms [25], we examined the effect of BMSC-CM as an alternative treatment for SCI.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have mainly focused on controlling and limiting the mechanisms involved with protecting the nerves against secondary damage in the affected area [6]. Replacing lost neurons and promoting neuronal regeneration using stem cells and their derivatives, such as stem cellconditioned medium (CM), has been extensively studied [7,8]. Breast milk is a valuable and noninvasive source of heterogeneous stem cells.…”

Section: Introductionmentioning

confidence: 99%

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The Therapeutic Potential of Conditioned Medium from Human Breast Milk Stem Cells in Treating Spinal Cord Injury

2020

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Study Design: Experimental animal study.Purpose: This study investigated the therapeutic effects of human breast milk stem cell (BMSC)-conditioned medium (BMSC-CM) in a model of spinal cord injury (SCI) in male Sprague-Dawley rats.Overview of Literature: SCI is one of the leading causes of disability in addition to sensory and motor impairment. So far, there have been no successful treatments for SCI. Given the positive outcomes associated with using stem cells and their derivatives as a treatment for various diseases, there is a growing interest in using them as an SCI treatment. Recent research has demonstrated that CM from stem cells has therapeutic advantages.Methods: Human BMSCs were isolated and characterized, and CM was subsequently collected. Animals received an intrathecal administration of BMSC-CM after SCI. The activity of caspase-3 was measured to assess apoptosis, and levels of tumor necrosis factor-α and interleukin-1β were measured to assess inflammation. Also, sensory and locomotor performances were assessed after SCI and BMSC-CM administration.Results: Administration of CM from BMSC reduced apoptosis and inflammation at the site of injury in a rat model of SCI (p<0.05). Motor, sensory, locomotor, and sensorimotor performances were significantly improved in rats that received BMSC-CM after SCI.Conclusions: Intrathecal administration of BMSC-CM improved recovery in a rat model of SCI.

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“…Currently, beside surgical intervention, the common approach to treat SCI is applied large doses of steroids, such as methylprednisolone. However, this treatment has been largely discontinued due to its time sensitivity and the severe side effects [34]. A growing body of evidence indicate that BMSCs can ameliorate motor functions in animal models of SCI by regulating diverse pathophysiological events including oxidative stress, apoptosis and in ammatory reactions [35].…”

Section: Discussionmentioning

confidence: 99%

Transplantation of Neuregulin1-Overexpressing Bone Marrow Mesenchymal Cells Accelerates Motor Functional Recovery by Facilitating Neurite Outgrowth and Reducing Cell Apoptosis in Rat after Spinal Cord Injury

Wu¹,

Liu²,

Zhu³

et al. 2020

Preprint

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Background: Bone marrow mesenchymal stem cells (BMSCs) transplantation offers an attractive strategy for treating multiply neurological diseases. Neuregulin1 (NRG1) plays fundamental roles in nervous system development and nerve repair. In this study, we aimed to investigate whether transplantation of NRG1-overexpressing BMSCs could alleviate spinal cord injury (SCI), and to explore the possible underling mechanisms. Methods: In vitro, NRG1-overexpressing BMSCs were constructed via plasmid transfection, and co-cultured with PC12 cells subjected to oxygen-glucose deprivation (OGD). Neurite outgrowth, cell viability and apoptosis of PC12 cells were evaluated. In vivo, BMSCs, empty-vector BMSCs and NRG1-overexpressing BMSCs were transplanted respectively into rats with SCI. Rat locomotor functions, neuronal chromatolysis, neurite outgrowth and cell apoptosis were assessed respectively. Results: The results showed that NRG1-overexpressing BMSCs in vitro significantly expedited neurite growth, elevated growth-associated protein 43 expression, enhanced cell viability and rescued ODG-induced apoptosis in PC12 cells. In vivo, transplantation of NRG1-overexpressing BMSCs notably accelerated rat motor functional recovery, attenuated neuronal chromatolysis, promoted neurite outgrowth and reduced cell apoptosis after SCI. Moreover, NRG1-overexpressing BMSCs were also able to regulate apoptosis-related proteins expression after SCI. Conclusions: These findings demonstrate that NRG1-overexpressing BMSCs can accelerate motor functional recovery by facilitating neurite outgrowth and reducing cell apoptosis after SCI, suggesting that NRG1-overexpressing BMSCs may be a promising candidate for the treatment of SCI.

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Neural Stem Cell-Conditioned Medium Suppresses Inflammation and Promotes Spinal Cord Injury Recovery

Cited by 56 publications

References 55 publications

Stem cell transplantation and functional recovery after spinal cord injury: a systematic review and meta-analysis

Stem cell transplantation and functional recovery after spinal cord injury: a systematic review and meta-analysis

The Therapeutic Potential of Conditioned Medium from Human Breast Milk Stem Cells in Treating Spinal Cord Injury

Transplantation of Neuregulin1-Overexpressing Bone Marrow Mesenchymal Cells Accelerates Motor Functional Recovery by Facilitating Neurite Outgrowth and Reducing Cell Apoptosis in Rat after Spinal Cord Injury

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