Neural stem cells express RET, produce nitric oxide, and survive transplantation in the gastrointestinal tract

Micci, Maria‐Adelaide; Learish, Randall D.; Li, Hui; Abraham, Bincy; Pasricha, Pankaj J.

doi:10.1053/gast.2001.28633

Cited by 57 publications

(50 citation statements)

References 28 publications

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“…167, NO. 2, MAY 15, 2011 e228 [5,10,11,22]. However, BrdU can yield false positive results due to the incorporation of BrdU from lysed dead cells or otherwise directly from the medium [2].…”

Section: Colonic Smooth Muscle Contraction Induced By Acetylcholinementioning

confidence: 99%

“…A variety of stem cells or neural progenitors derived from the gut have been reported to generate neurospheres and differentiate into neurons and glial cells with characteristic ENS phenotypes in vitro [2][3][4][5][6][7][8][9][10][11]. In addition, delivery of neural progenitors into the gut has been shown to improve bowel function [4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transplantation of Neonatal Gut Neural Crest Progenitors Reconstructs Ganglionic Function in Benzalkonium Chloride-Treated Homogenic Rat Colon

Pan

Zheng

Gao

et al. 2011

Journal of Surgical Research

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Section: Colonic Smooth Muscle Contraction Induced By Acetylcholinementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transplantation of Neonatal Gut Neural Crest Progenitors Reconstructs Ganglionic Function in Benzalkonium Chloride-Treated Homogenic Rat Colon

Pan

Zheng

Gao

et al. 2011

Journal of Surgical Research

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“…Recent work has shown that neural stem cells from other sources grafted into the stomach can integrate into the ENS and restore some function (Micci et al 2005a;Micci et al 2001;Micci et al 2005b), see (Micci and Pasricha 2007). Understanding the biology of resident enteric neural stem cells is therefore important, to optimize the outcome of such potentially valuable therapeutic approaches.…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Neural progenitors from isolated postnatal rat myenteric ganglia: Expansion as neurospheres and differentiation in vitro

et al. 2008

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Identification of the stem cell niche is crucial for understanding the factors that regulate these cells. Rodent enteric neural crest-derived stem cells have previously been isolated by flow cytometry and culture of cell suspensions from the outer smooth muscle layers or the entire gut wall from postnatal and adult animals. Such cell suspensions contain a mixture of cell types, including smooth muscle, fibroblasts and cells associated with the vasculature and extrinsic innervation. Thus these preparations may be contaminated by stem cells associated with extrinsic sensory and autonomic nerves and by other types of stem cell that reside in the gut. Here we describe a different approach, similar to that recently used for infant human gut, to obtain enteric ganglionderived cells, with properties of neural progenitor cells, using isolated myenteric ganglia from postnatal rat ileum. Myenteric ganglia were separated from the gut wall, dispersed and resulting cell dissociates were plated in non-adherent culture conditions with EGF and FGF-2. Under these conditions neurospherelike bodies (NLB) developed. Cells in NLB incorporated BrdU and expressed the stem cell marker nestin but not the pan-neuronal marker PGP 9.5. Upon growth factor withdrawal some BrdU-immunopositive cells assumed the morphology of neurons and expressed PGP 9.5; others were flattened and expressed the glial cell marker GFAP. This work therefore provides evidence that neural crest-derived progenitors in the postnatal rat gut are located in the myenteric plexus, and shows that these cells can be expanded and differentiated in NLB in vitro.

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“…Transplantation of neural stem cells may hold potential to treat disorders of the central and peripheral nervous systems [1][2][3][4][5][6][7]. Hirschsprung's disease, achalasia, and other motility disorders may be treatable with advances in stem cell transplantation [1,2].…”

Section: Introductionmentioning

confidence: 99%

“…Brain-derived neural stem cells transplanted in the gut survived and expressed enteric markers; however, they may not provide a feasible source [1][2][3][4][5][6][7]. NCSCs isolated from the gut showed enhanced migration and expression of enteric markers in the gut compared with sciatic nerve NCSCs [26].…”

Section: Introductionmentioning

confidence: 99%

Transplantation of Enteric Cells Expressing p75 in the Rodent Stomach

Geisbauer

Chapin

et al. 2012

Journal of Surgical Research

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Neural stem cells express RET, produce nitric oxide, and survive transplantation in the gastrointestinal tract

Cited by 57 publications

References 28 publications

Transplantation of Neonatal Gut Neural Crest Progenitors Reconstructs Ganglionic Function in Benzalkonium Chloride-Treated Homogenic Rat Colon

Transplantation of Neonatal Gut Neural Crest Progenitors Reconstructs Ganglionic Function in Benzalkonium Chloride-Treated Homogenic Rat Colon

Neural progenitors from isolated postnatal rat myenteric ganglia: Expansion as neurospheres and differentiation in vitro

Transplantation of Enteric Cells Expressing p75 in the Rodent Stomach

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