Neural tube patterning by Ephrin, FGF and Notch signaling relays

Stolfi, Alberto; Wagner, Eileen; Taliaferro, J. Matthew; Chou, Seemay; Levine, Michael

doi:10.1242/dev.072108

Cited by 58 publications

(80 citation statements)

References 74 publications

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“…Adult mice lacking ephrin-A2 and -A3 display active ongoing neurogenesis throughout the CNS. 73 Interactions of ephrin-A/EphA family with neurogenic signals, such as Wnt and FGF, have been documented [74][75][76] , further supporting a role of ephrin-As in the regulation of neural stem cell behavior. Recent reports indicate that ephrin-As also play a key inhibitory role in the developing retina and adult ciliary epithelium to suppress stem cell proliferation and retinogenesis via suppressing Wnt signaling 77 .…”

Section: Niche Signals and Stem Cell Potentialmentioning

confidence: 89%

Mobilizing Endogenous Stem Cells for Retinal Repair

Yu¹,

Talib²,

Vu³

et al. 2016

Translating Regenerative Medicine to the Clinic

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Section: Niche Signals and Stem Cell Potentialmentioning

confidence: 89%

Mobilizing Endogenous Stem Cells for Retinal Repair

Yu¹,

Talib²,

Vu³

et al. 2016

Translating Regenerative Medicine to the Clinic

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“…Dig-labelled probes were synthesised from the following cDNA clones: Bra (Corbo et al, 1997), ETR (Hudson et al, 2003), Mnx (Stolfi et al, 2011), NoTrlc (citb018l16) and Titf (ciad042d09). Images in Fig.…”

Section: In Situ Hybridisation and Immunohistochemistrymentioning

confidence: 99%

“…For immunodetection of diphosphorylated (dp) ERK1/2, the protocol described previously (Stolfi et al, 2011) was used with slight modifications. Embryos were fixed in 1 ml PIPES-sucrose-FA buffer for 30 minutes at room temperature with constant rotation.…”

Section: In Situ Hybridisation and Immunohistochemistrymentioning

confidence: 99%

“…RESULTS AND DISCUSSION p120RasGAP is required for correct fate specification during marginal zone and motor ganglion patterning A requirement for ephrin/Eph-mediated attenuation of FGF/ERK signals has been shown for several binary fate decisions during embryonic patterning of ascidian embryos (Picco et al, 2007;Shi and Levine, 2008;Stolfi et al, 2011). Two such examples are found during patterning of the marginal zone.…”

Section: Western Blot and Co-immunoprecipitationmentioning

confidence: 99%

“…In Ciona intestinalis, several cases of differential ERK activation between sister cells have been described during marginal zone and neural lineage patterning. This differential ERK activation between sister cell pairs dictates their differential fate specification Stolfi et al, 2011;Wagner and Levine, 2012;Yasuo and Hudson, 2007) (reviewed by Lemaire, 2009). Importantly, in several of these cases, differential ERK activation is achieved via ephrin/Eph-dependent attenuation of FGF/ERK signalling (Picco et al, 2007;Shi and Levine, 2008;Stolfi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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p120RasGAP mediates ephrin/Eph-dependent attenuation of FGF/ERK signals during cell fate specification in ascidian embryos

et al. 2013

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SUMMARYERK1/2 MAP kinase exhibits a highly dynamic activation pattern in developing embryos, which largely depends on fibroblast growth factor (FGF) signals. In ascidian embryos, FGF-dependent activation of ERK1/2 occurs differentially between sister cells during marginal zone and neural lineage patterning. Selective attenuation of FGF signals by localised ephrin/Eph signals accounts for this differential ERK activation, which controls the binary fate choice of each sibling cell pair. Here, we show that p120 Ras GTPase-activating protein (p120RasGAP) is a crucial mediator of these ephrin/Eph signals. First, inhibition of p120RasGAP has a similar effect to inhibition of ephrin/Eph function during marginal zone and neural patterning. Second, p120RasGAP acts epistatically to ephrin/Eph signals. Third, p120RasGAP physically associates with Eph3 in an ephrin-dependent manner. This study provides the first in vivo evidence that the functional association between Eph and RasGAP controls the spatial extent of FGF-activated ERK.

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Quantitative and in toto imaging in ascidians: Working toward an image‐centric systems biology of chordate morphogenesis

Veeman

Reeves

2014

Genesis

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Developmental biology relies heavily on microscopy to image the finely-controlled cell behaviors that drive embryonic development. Most embryos are large enough that a field of view with the resolution and magnification needed to resolve single cells will not span more than a small region of the embryo. Ascidian embryos, however, are sufficiently small that they can be imaged in toto with fine subcellular detail using conventional microscopes and objectives. Unlike other model organisms with particularly small embryos, ascidians have a chordate embryonic body plan that includes a notochord, hollow dorsal neural tube, heart primordium and numerous other anatomical details conserved with the vertebrates. Here we compare the size and anatomy of ascidian embryos with those of more traditional model organisms, and relate these features to the capabilities of both conventional and exotic imaging methods. We review the emergence of Ciona and related ascidian species as model organisms for a new era of image-based developmental systems biology. We conclude by discussing some important challenges in ascidian imaging and image analysis that remain to be solved.

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Neural tube patterning by Ephrin, FGF and Notch signaling relays

Abstract: There was an error published in Development 138, 5429-5439.On p. 5431, PIPES-sucrose-FA buffer was incorrectly described. The correct composition of this buffer is: 400 mM sucrose, 50 mM EGTA, 100 mM PIPES pH 7, 4% formaldehyde.The authors apologise to readers for this mistake.

Cited by 58 publications

References 74 publications

Mobilizing Endogenous Stem Cells for Retinal Repair

Mobilizing Endogenous Stem Cells for Retinal Repair

p120RasGAP mediates ephrin/Eph-dependent attenuation of FGF/ERK signals during cell fate specification in ascidian embryos

Quantitative and in toto imaging in ascidians: Working toward an image‐centric systems biology of chordate morphogenesis

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