Neuraminidase produces dose-dependent decrease of slime production and adherence of slime-forming, coagulase-negative staphylococci

Sakarya, Serhan; Öncü, Serkan; Öncü, Selcen; Öztürk, Barçın; Tuncer, Günay Ertem; Sari, Cavide

doi:10.1016/j.arcmed.2004.03.008

Cited by 9 publications

(4 citation statements)

References 30 publications

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“…Data suggested involvement of protein components in adherence to polystyrene, but not in autoaggregation properties and adherence to glass surfaces. Previous studies also gave an insight into the mechanism of slime production and adherence of slime-forming CoNS to polystyrene plates and polypropylene tubes (Sakarya et al, 2004). Biofilm formation and adherence to acrylic by S. haemolyticus were found significantly reduced compared to those of S. epidermidis (Cerca et al, 2004(Cerca et al, , 2005.…”

Section: Discussionmentioning

confidence: 94%

“…Other materials such as polypropylene, polystyrene, vinyl (PVC), silicon, Teflon, and silicone tend to collect electrons and become negatively charged. Sialic acid may be a constituent molecule of slime and involved in bacterial adherence to inert surfaces, as demonstrated for Corynebacterium diphtheriae (Mattos-Guaraldi et al, 1999) and CoNS (Sakarya et al, 2004). Krepsky et al (2003) investigated cell surface hydrophobicity and slime production of S. epidermidis.…”

Section: Discussionmentioning

confidence: 99%

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Staphylococcus haemolyticus disseminated among neonates with bacteremia in a neonatal intensive care unit in Rio de Janeiro, Brazil

Pereira

Binatti

Sued

et al. 2014

Diagnostic Microbiology and Infectious Disease

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Oxacillin-resistant Staphylococcus haemolyticus (ORSH) was found as the most prevalent (77.5%) species of coagulase-negative staphylococci associated with bacteremia in neonates making use of intravenous catheters in an intensive care unit of a Brazilian teaching hospital. Thirty-one blood isolates were confirmed as S. haemolyticus by sequencing of the 16S and clustered in 6 pulsed-field gel electrophoresis types (with 58% of the strains belonging to 2 predominant types B and D). S. haemolyticus was mostly oxacillin-resistant (90.3%) displaying multiresistance profiles (70.4%). However, the mecA gene was undetected in 22.6% strains. ORSH exhibited slime production on Congo-Red agar (67.7%), adherence to polystyrene (96.7%), and glass (87%) surfaces. Interestingly, ica-operon was detected in 58% strains, mostly belonging to the B, D, and F genotypes, which is a significantly higher percentage when compared to other studies conducted at different parts of the globe. Data indicated that ica operon and biofilm-forming ORSH are endemic in Brazilian nosocomial environment.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Staphylococcus haemolyticus disseminated among neonates with bacteremia in a neonatal intensive care unit in Rio de Janeiro, Brazil

Pereira

Binatti

Sued

et al. 2014

Diagnostic Microbiology and Infectious Disease

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“…The difference seen in our study may result from in vitro circumstances. The ability of S. aureus to form a biofilm, sialic acid-forming slime, bacterial enzymes increasing adhesion, the molecular properties of the bacteria, and the graft all play important roles in adhesion [14,18,29], but we do not know which effects are greater in vitro or in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…The virulence of S. aureus and E. coli depends on the patient's tissue, structural and molecular properties of the prostheses, secretory products, and the surface properties of the bacteria [14][15][16]. Several studies have examined the in vivo and in vitro interactions between prostheses and bacteria [15,17,18].…”

Section: Original Articlementioning

confidence: 99%

Comparison of the adherence of E.Coli and S. Aureus to ten different prosthetic mesh grafts: In vitro experimental study

et al. 2010

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Neuraminidase decreases in vitro adherence of slime-forming coagulase-negative staphylococci to biosynthetic ovine collagen vascular graft

Önem

Saçar

et al. 2006

Adv Therapy

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Vascular prosthetic graft infection is a major complication of vascular surgery that starts with adhesion of the microorganism to the graft. Because slime-forming microorganisms are the major causative agents in graft infection, the goals of investigators in this study were (1) to investigate the bacterial adherence of slime-forming and non-slime-forming coagulase-negative staphylococci (CNS), and (2) to determine the role of neuraminidase (NANase) in bacterial adherence to the biosynthetic ovine collagen graft. Human plasma was instilled and incubated at 37 degrees C in preparation for fibrin deposition of grafts. After 48 hours, incubation grafts were drained and inoculated with slime-forming and non-slime-forming CNS in tryptic soy broth in the presence and in the absence of neuraminidase. After 24 hours of incubation at 36 degrees C, grafts were vortexed and cultured for colony count. Bacterial counts were expressed as total colony-forming units per longitudinal centimeter of the graft. Slime-forming CNS had greater affinity to the collagen graft compared with non-slime-forming CNS (P<.05). Adherence of slime-forming CNS was impaired by NANase treatment (P<.001). NANase treatment of patients with non-slime-forming CNS did not change adherence to the graft (P>.05). Results show that slime plays an important role in the pathogenesis of vascular graft infection. Adherence of slime-forming CNS can be decreased through the administration of NANase. This may have implications for the development of neuraminidase-embedded vascular grafts designed to reduce the occurrence of biomaterial-related infection.

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Neuraminidase produces dose-dependent decrease of slime production and adherence of slime-forming, coagulase-negative staphylococci

Cited by 9 publications

References 30 publications

Staphylococcus haemolyticus disseminated among neonates with bacteremia in a neonatal intensive care unit in Rio de Janeiro, Brazil

Staphylococcus haemolyticus disseminated among neonates with bacteremia in a neonatal intensive care unit in Rio de Janeiro, Brazil

Comparison of the adherence of E.Coli and S. Aureus to ten different prosthetic mesh grafts: In vitro experimental study

Neuraminidase decreases in vitro adherence of slime-forming coagulase-negative staphylococci to biosynthetic ovine collagen vascular graft

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