Neuregulin1 administration increases axonal elongation in dissociated primary sensory neuron cultures

Audisio, Chiara; Mantovani, Cristina; Raimondo, Stefania; Geuna, Stefano; Perroteau, Isabelle; Terenghi, Giorgio

doi:10.1016/j.yexcr.2012.01.011

Cited by 14 publications

(8 citation statements)

References 41 publications

(68 reference statements)

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“…The length of the longest neurite was greater in SM compared with CM. Heregulin in SM might enhance the neurite outgrowth of NSC34 cells [26]. Among the other factors that are present in SM, bFGF is also known to enhance neurite outgrowth by stimulating the MEK-ERK1/2 and PI3K-AKT pathways [27].…”

Section: Resultsmentioning

confidence: 99%

Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration

Jung

Park

Choi

et al. 2016

IJMS

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Schwann cells (SCs), which produce neurotropic factors and adhesive molecules, have been reported previously to contribute to structural support and guidance during axonal regeneration; therefore, they are potentially a crucial target in the restoration of injured nervous tissues. Autologous SC transplantation has been performed and has shown promising clinical results for treating nerve injuries and donor site morbidity, and insufficient production of the cells have been considered as a major issue. Here, we performed differentiation of tonsil-derived mesenchymal stem cells (T-MSCs) into SC-like cells (T-MSC-SCs), to evaluate T-MSC-SCs as an alternative to SCs. Using SC markers such as CAD19, GFAP, MBP, NGFR, S100B, and KROX20 during quantitative real-time PCR we detected the upregulation of NGFR, S100B, and KROX20 and the downregulation of CAD19 and MBP at the fully differentiated stage. Furthermore, we found myelination of axons when differentiated SCs were cocultured with mouse dorsal root ganglion neurons. The application of T-MSC-SCs to a mouse model of sciatic nerve injury produced marked improvements in gait and promoted regeneration of damaged nerves. Thus, the transplantation of human T-MSCs might be suitable for assisting in peripheral nerve regeneration.

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Section: Resultsmentioning

confidence: 99%

Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration

Jung

Park

Choi

et al. 2016

IJMS

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“…It has been reported that neurotrophic factors provide neural survival following damage and that there is a direct association between exogenous neurotrophic factor application and axonal regeneration (22,49). In the evaluation of peripheral nerve regeneration, total number of axons in the distal part of nerve and functional tests are used (50)(51)(52).…”

Section: Regenerative Events Following Damagementioning

confidence: 99%

The role of growth factors in nerve regeneration

Önger

Delibaş

Türkmen

et al. 2016

DD&T

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“…Similar results have been described for PNS cell cultures when DRG cells were grown on biomimetic scaffolds, although in this case, the maximal average neurite length described was 230 ± 30 μm. 10 Considering that DRG cells have been widely used as a model for in vitro nerve regeneration, because of their ability to produce axonal elongation compared to CNS cells, 49 the neurite lengths observed when CNS cortical neurons were grown on SFS fibers confirmed the optimal characteristics of these fibers to be used as scaffolds for nerve regeneration applications.…”

Section: Discussionmentioning

confidence: 95%

Regenerated Silk Fibers Obtained by Straining Flow Spinning for Guiding Axonal Elongation in Primary Cortical Neurons

Mercado

Pérez‐Rigueiro

González‐Nieto

et al. 2020

ACS Biomater. Sci. Eng.

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The recovery of injured nervous tissue, one of the main goals for regenerative therapeutic approaches, is often hindered by the limited axonal regeneration ability of the central nervous system (CNS). In this regard, the identification of scaffolds that support the reconstruction of functional neuronal tissues and guide the alignment of regenerating neurons is a major challenge in tissue engineering. Ideally, the usage of such scaffolds would promote and guide the axonal growth, a crucial phase for the restoration of neuronal connections and, consequently, the nerve function. Among the materials proposed as scaffolds for CNS regeneration, silk has been used to exploit its outstanding features as a biomaterial to promote axonal regeneration. In this study, we explore, for the first time, the possibility of using high-performance regenerated silk fibers obtained by straining flow spinning (SFS) to serve as scaffolds for inducing and guiding the axonal growth. It is shown that SFS fibers promote the spontaneous organization of dissociated cortical primary cells into highly interconnected cellular spheroid-like tissue formations. Neuronal projections (i.e., axons) from these cellular spheroids span hundreds of microns along the SFS fibers that act as guides and allow the connection of distant spheroids. In addition, it is also shown that SFS fibers serve as scaffolds for neuronal migration covering short and long distances. As a consequence, the usage of high-performance SFS fibers appears as a promising basis for the development of novel therapies, leading to directed axonal regeneration.

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Neuregulin1 administration increases axonal elongation in dissociated primary sensory neuron cultures

Cited by 14 publications

References 41 publications

Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration

Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration

The role of growth factors in nerve regeneration

Regenerated Silk Fibers Obtained by Straining Flow Spinning for Guiding Axonal Elongation in Primary Cortical Neurons

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