Neuregulinas 1-alfa e 1-beta na regeneração de nervos periféricos

Souza, Fabiano Inácio de; Zumiotti, Arnaldo Valdir; Silva, Ciro Ferreira da

doi:10.1590/s1413-78522010000500003

Cited by 5 publications

(1 citation statement)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The trend is similar also for NRG1β, but its expression is lower. These data are highly reminiscent of our previous observation (carried out on the distal portion of different experimental rat models: Crush injury and cut followed by end-to-end repair) where we found that NRG1α isoforms are more highly upregulated than NRG1β isoforms [17], thus reinforcing the idea that α isoforms also might play a role in nerve regeneration [50].…”

Section: Discussionsupporting

confidence: 87%

Fibroblasts Colonizing Nerve Conduits Express High Levels of Soluble Neuregulin1, a Factor Promoting Schwann Cell Dedifferentiation

Fornasari

Soury

Nato

et al. 2020

Cells

View full text Add to dashboard Cite

Conduits for the repair of peripheral nerve gaps are a good alternative to autografts as they provide a protected environment and a physical guide for axonal re-growth. Conduits require colonization by cells involved in nerve regeneration (Schwann cells, fibroblasts, endothelial cells, macrophages) while in the autograft many cells are resident and just need to be activated. Since it is known that soluble Neuregulin1 (sNRG1) is released after injury and plays an important role activating Schwann cell dedifferentiation, its expression level was investigated in early regeneration steps (7, 14, 28 days) inside a 10 mm chitosan conduit used to repair median nerve gaps in Wistar rats. In vivo data show that sNRG1, mainly the isoform α, is highly expressed in the conduit, together with a fibroblast marker, while Schwann cell markers, including NRG1 receptors, were not. Primary culture analysis shows that nerve fibroblasts, unlike Schwann cells, express high NRG1α levels, while both express NRG1β. These data suggest that sNRG1 might be mainly expressed by fibroblasts colonizing nerve conduit before Schwann cells. Immunohistochemistry analysis confirmed NRG1 and fibroblast marker co-localization. These results suggest that fibroblasts, releasing sNRG1, might promote Schwann cell dedifferentiation to a “repair” phenotype, contributing to peripheral nerve regeneration.

show abstract

Section: Discussionsupporting

confidence: 87%