Neurobiology of the gustatory systems ofDrosophila and some terrestrial insects

Singh, Ranjana

doi:10.1002/(sici)1097-0029(19971215)39:6<547::aid-jemt7>3.0.co;2-a

Cited by 97 publications

(75 citation statements)

References 129 publications

(165 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The larval head chemosensory system includes three pharyngeal organs [dorsal pharyngeal organ (DPS), ventral pharyngeal organ (VPS), and posterior pharyngeal organs (PPS)] that may serve gustation and mechanosensation (Singh 1997), as well as three external sensory organs, the dorsal organ (DO), the terminal organ (TO), and the ventral organ (VO) organs. Each of these organs consists of several sensilla, with each sensillum comprising one to nine sensory neurons and three accessory cells.…”

Section: Atk Localizes To the Supporting Ecm Of Type I Sensory Organsmentioning

confidence: 99%

The Extracellular Matrix Protein Artichoke Is Required for Integrity of Ciliated Mechanosensory and Chemosensory Organs inDrosophilaEmbryos

et al. 2014

View full text Add to dashboard Cite

Sensory cilia are often encapsulated by an extracellular matrix (ECM). In Caenorhabditis elegans, Drosophila melanogaster, and vertebrates, this ECM is thought to be directly involved in ciliary mechanosensing by coupling external forces to the ciliary membrane. Drosophila mechano-and chemosensory cilia are both associated with an ECM, indicating that the ECM may have additional roles that go beyond mechanosensory cilium function. Here, we identify Artichoke (ATK), an evolutionarily conserved leucine-rich repeat ECM protein that is required for normal morphogenesis and function of ciliated sensilla in Drosophila. atk is transiently expressed in accessory cells in all ciliated sensory organs during their late embryonic development. Antibody stainings show ATK protein in the ECM that surrounds sensory cilia. Loss of ATK protein in atk null mutants leads to cilium deformation and disorientation in chordotonal organs, apparently without uncoupling the cilia from the ECM, and consequently to locomotion defects. Moreover, impaired chemotaxis in atk mutant larvae suggests that, based on ATK protein localization, the ECM is also crucial for the correct assembly of chemosensory receptors. In addition to defining a novel ECM component, our findings show the importance of ECM integrity for the proper morphogenesis of ciliated organs in different sensory modalities.

show abstract

Section: Atk Localizes To the Supporting Ecm Of Type I Sensory Organsmentioning

confidence: 99%

The Extracellular Matrix Protein Artichoke Is Required for Integrity of Ciliated Mechanosensory and Chemosensory Organs inDrosophilaEmbryos

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Two additional groups of neurons labeled with GFP innervate the labral sense organs (LSO) (Stocker, 1994;Mitchell et al, 1999). This paired organ of the internal mouth parts is composed of six mechanosensory and eight chemosensory neurons (Singh, 1997), about six of which express GFP (Fig. 3D) driven by the Poxn-Gal4 constructs (Fig.…”

Section: Late Enhancer Active In Chemosensory Neurons Of All Taste Brmentioning

confidence: 99%

TheDrosophila Pox neurogene: control of male courtship behavior and fertility as revealed by a complete dissection of all enhancers

2002

View full text Add to dashboard Cite

show abstract

“…Interestingly, the adult pharynx derives essentially from small, densely packed imaginal cells -the clypeolabral bud -that are closely associated with the larval pharyngeal skeleton (Bryant, 1978;Gehring and Seippel, 1967;Struhl, 1981). Does this imply that adult pharyngeal sensilla are born during metamorphosis, like their external counterparts, or do the anatomical similarities of certain larval (Python and Stocker, 2002;Singh, 1997;Singh and Singh, 1984) and adult pharyngeal organs (Nayak and Singh, 1983;Stocker, 1994;Stocker and Schorderet, 1981) rather suggest persistence of sensilla through metamorphosis?…”

Section: Introductionmentioning

confidence: 99%

Integration of complex larval chemosensory organs into the adult nervous system ofDrosophila

et al. 2004

View full text Add to dashboard Cite

The sense organs of adult Drosophila, and holometabolous insects in general, derive essentially from imaginal discs and hence are adult specific. Experimental evidence presented here, however, suggests a different developmental design for the three largely gustatory sense organs located along the pharynx. In a comprehensive cellular analysis, we show that the posteriormost of the three organs derives directly from a similar larval organ and that the two other organs arise by splitting of a second larval organ. Interestingly, these two larval organs persist despite extensive reorganization of the pharynx. Thus, most of the neurons of the three adult organs are surviving larval neurons. However, the anterior organ includes some sensilla that are generated during pupal stages. Also, we observe apoptosis in a third larval pharyngeal organ. Hence, our experimental data show for the first time the integration of complex, fully differentiated larval sense organs into the nervous system of the adult fly and demonstrate the embryonic origin of their neurons. Moreover, they identify metamorphosis of this sensory system as a complex process involving neuronal persistence, generation of additional neurons and neuronal death. Our conclusions are based on combined analysis of reporter expression from P[GAL4] driver lines, horseradish peroxidase injections into blastoderm stage embryos, cell labeling via heat-shock-induced flip-out in the embryo, bromodeoxyuridine birth dating and staining for programmed cell death. They challenge the general view that sense organs are replaced during metamorphosis.

show abstract

Neurobiology of the gustatory systems ofDrosophila and some terrestrial insects

Cited by 97 publications

References 129 publications

The Extracellular Matrix Protein Artichoke Is Required for Integrity of Ciliated Mechanosensory and Chemosensory Organs inDrosophilaEmbryos

The Extracellular Matrix Protein Artichoke Is Required for Integrity of Ciliated Mechanosensory and Chemosensory Organs inDrosophilaEmbryos

TheDrosophila Pox neurogene: control of male courtship behavior and fertility as revealed by a complete dissection of all enhancers

Integration of complex larval chemosensory organs into the adult nervous system ofDrosophila

Contact Info

Product

Resources

About