Neuroblast ablation in Drosophila P[GAL4] lines reveals origins of olfactory interneurons

Abstract

Hydroxyurea (HU) treatment of on targets in the calyx. Hence, the ablated interneuearly first instar larvae in Drosophila was previously rons were derived from the LNb, whereas the HUshown to ablate a single dividing lateral neuroblast (LNb) in the brain. Early larval HU application to resistant elements originated from neuroblasts which P[GAL4] strains that label specific neuron types enbegin to divide later in larval life. Developmental abled us to identify the origins of the two major classes GAL4 expression patterns suggested that differentiof interneurons in the olfactory system. HU treatment ated RI are present at the larval stage already and resulted in the loss of antennal lobe local interneurons may be retained through metamorphosis. ᭧ 1997 John and of a subset of relay interneurons (RI), elements Wiley & Sons, Inc. J Neurobiol 32: 443-456, 1997 usually projecting to the calyx and the lateral protoc-Keywords: olfactory interneurons; cell lineage; pererebrum (LPR). Other RI were resistant to HU and sisting larval neurons; P[GAL4] enhancer trap lines; still projected to the LPR. However, they formed no hydroxyurea ablation; Drosophila melanogaster collaterals in the calyx region (which was also ab-

Diversity and expression of odorant receptors in Drosophila

“…To answer this question, two groups performed sophisticated anatomical tracing experiments to trace the projections of single projection neurons whose dendrites innervate a given glomerulus (Marin et al, 2002;Wong et al, 2002). To accomplish this, they made us of an enhancer trap line that expresses Gal4 in a large number of the projections neurons that surround the antennal lobe, as well as some unrelated neurons (Stocker et al, 1997). Using various techniques to generate somatic mosaic clones in which only a single of these projections neurons now expresses Gal4, both groups were able to label these neurons with GFP variants that revealed the morphology of the entire neuron from dendrite to axon.…”

Target neuron prespecification in the olfactory map of Drosophila

“…To study projection neurons of the antennal lobe, we made use of a GAL4 line, GAL4-GH146 (ref. 14), which drives marker expression in a large subset of projection neurons (approximately 90). By crossing GH146 with a membrane marker, UAS-mouse CD8-green uorescent protein (GFP) 5 , we can visualize the cell body and dendrites of projection neurons in the antennal lobe in the anterior part of the brain (Fig.…”

DmOAZ, the unique Drosophila melanogaster OAZ homologue is involved in posterior spiracle development

“…By remobilizing the transposable P[GawB] of GH146-Gal4 (Stocker et al 1997;Heimbeck et al 2001), which is located just 280 bp upstream of the DmOAZ start codon, we created a number of mutant lines. From an initial 180 strains, no viable mutation in dmOAZ was recovered, arguing that this locus plays an important role in viability.…”

“…The CyO + phenotype was used to identify trans-heterozygous individuals resulting from the cross (n>100 adults scored in all cases). dmOAZ mutants were generated by remobilizing the P[Gal4] element of the GH146 enhancer trap line (Stocker et al 1997;Heimbeck et al 2001).…”

“…The P[GawB] transposable element of the GH146-Gal4 enhancer trap line (Stocker et al 1997;Heimbeck et al 2001) is inserted close to the dmOAZ map position at 51A3-4. We cloned and sequenced the P element-flanking sequences by inverse PCR (Dalby et al 1995).…”