Neuroblast Migration and P2Y<sub>1</sub>Receptor Mediated Calcium Signalling Depend on 9-O-Acetyl GD3 Ganglioside

Santiago, Marcelo F.; Scemes, Eliana

doi:10.1042/an20120035

Cited by 13 publications

(11 citation statements)

References 62 publications

(96 reference statements)

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“…Furthermore, postnatal granule cell precursors of the cerebellum migrate and then differentiate into excitatory granule cells and integrate into the cerebellar circuitry (Ito, ). The antagonism of P2Y1Rs and the immunoblockade of gangliosides similarly reduced the frequency of Ca 2+ oscillations of granule cell precursors and their migration into the cerebellum (Santiago and Scemes, ).…”

Section: Proliferation Migration Differentiation and Necrosis/apopmentioning

confidence: 99%

Regulation of adult neural progenitor cell functions by purinergic signaling

Tang

Illéš

2016

Glia

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Extracellular purines are signaling molecules in the neurogenic niches of the brain and spinal cord, where they activate cell surface purinoceptors at embryonic neural stem cells (NSCs) and adult neural progenitor cells (NPCs). Although mRNA and protein are expressed at NSCs/NPCs for almost all subtypes of the nucleotide-sensitive P2X/P2Y, and the nucleoside-sensitive adenosine receptors, only a few of those have acquired functional significance. ATP is sequentially degraded by ecto-nucleotidases to ADP, AMP, and adenosine with agonistic properties for distinct receptor-classes. Nucleotides/nucleosides facilitate or inhibit NSC/NPC proliferation, migration and differentiation. The most ubiquitous effect of all agonists (especially of ATP and ADP) appears to be the facilitation of cell proliferation, usually through P2Y1Rs and sometimes through P2X7Rs. However, usually P2X7R activation causes necrosis/apoptosis of NPCs. Differentiation can be initiated by P2Y2R-activation or P2X7R-blockade. A key element in the transduction mechanism of either receptor is the increase of the intracellular free Ca concentration, which may arise due to its release from intracellular storage sites (G protein-coupling; P2Y) or due to its passage through the receptor-channel itself from the extracellular space (ATP-gated ion channel; P2X). Further research is needed to clarify how purinergic signaling controls NSC/NPC fate and how the balance between the quiescent and activated states is established with fine and dynamic regulation. GLIA 2017;65:213-230.

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Section: Proliferation Migration Differentiation and Necrosis/apopmentioning

confidence: 99%

Regulation of adult neural progenitor cell functions by purinergic signaling

Tang

Illéš

2016

Glia

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“…influences the cellular distribution and function of the purinergic receptor P2Y(1)R, controlling the migration of cerebellar granule-cell progenitors (Santiago & Scemes, 2012). Future studies are necessary to determine whether similar developmental changes would also occur in the visual system of GD3s -/mice, being associated with the phenotype described here.…”

Section: Discussionmentioning

confidence: 76%

GD3 synthase deletion alters retinal structure and impairs visual function in mice

Abreu

Teixeira-Pinheiro

Lani-Louzada

et al. 2021

Journal of Neurochemistry

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Gangliosides are glycosphingolipids abundantly expressed in the vertebrate nervous system, and are classified into a‐, b‐, or c‐series according to the number of sialic acid residues. The enzyme GD3 synthase converts GM3 (an a‐series ganglioside) into GD3, a b‐series ganglioside highly expressed in the developing and adult retina. The present study evaluated the visual system of GD3 synthase knockout mice (GD3s–/–), morphologically and functionally. The absence of b‐ series gangliosides in the retinas of knockout animals was confirmed by mass spectrometry imaging, which also indicated an accumulation of a‐series gangliosides, such as GM3. Retinal ganglion cell (RGC) density was significantly reduced in GD3s–/– mice, with a similar reduction in the number of axons in the optic nerve. Knockout animals also showed a 15% reduction in the number of photoreceptor nuclei, but no difference in the bipolar cells. The area occupied by GFAP‐positive glial cells was smaller in GD3s–/– retinas, but the number of microglial cells/macrophages did not change. In addition to the morphological alterations, a 30% reduction in light responsiveness was detected through quantification of pS6‐expressing RGC, an indicator of neural activity. Furthermore, electroretinography (ERG) indicated a significant reduction in RGC and photoreceptor electrical activity in GD3s–/– mice, as indicated by scotopic ERG and pattern ERG (PERG) amplitudes. Finally, evaluation of the optomotor response demonstrated that GD3s–/– mice have reduced visual acuity and contrast sensitivity. These results suggest that b‐series gangliosides play a critical role in regulating the structure and function of the mouse visual system.

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“…The complete absence of staining in the SVZ of perfused (in which blood cells are absent in the brain) GD3 synthase-null mice, that exclusively lack all b- and c-series gangliosides [ 55 ], supports that hypothesis (data not shown). Moreover, we have recently shown that cerebellar progenitors cells extract from the same ganglioside null mice do not react at all with anti-9acGD3 antibodies [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…Immunoblockage of CD60b also inhibits the radial migration of cerebellar granule cells in the developing rat cerebellum [ 16 , 19 ] and tangential neuroblast migration from postnatal SVZ explants [ 20 ]. Furthermore, CD60b-dependent calcium signaling through purinergic receptors was described as crucial for the migration of granular cell precursors during development [ 21 ]. In the adult peripheral nervous system, CD60b is re-expressed in the sciatic nerve after a crush injury, and its expression correlates with the axonal outgrowth through the lesion site [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

CD60b: Enriching Neural Stem/Progenitor Cells from Rat Development into Adulthood

Gubert

Zaverucha-do-Valle

Furtado

et al. 2017

Stem Cells International

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CD60b antigens are highly expressed during development in the rat nervous system, while in the adult their expression is restricted to a few regions, including the subventricular zone (SVZ) around the lateral ventricles—a neurogenic niche in the adult brain. For this reason, we investigated whether the expression of C60b is associated with neural stem/progenitor cells in the SVZ, from development into adulthood. We performed in vitro and in vivo analyses of CD60b expression at different stages and identified the presence of these antigens in neural stem/progenitor cells. We also observed that CD60b could be used to purify and enrich a population of neurosphere-forming cells from the developing and adult brain. We showed that CD60b antigens (mainly corresponding to ganglioside 9-O-acetyl GD3, a well-known molecule expressed during central nervous system development and mainly associated with neuronal migration) are also present in less mature cells and could be used to identify and isolate neural stem/progenitor cells during development and in the adult brain. A better understanding of molecules associated with neurogenesis may contribute not only to improve the knowledge about the physiology of the mammalian central nervous system, but also to find new treatments for regenerating tissue after disease or brain injury.

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Neuroblast Migration and P2Y₁Receptor Mediated Calcium Signalling Depend on 9-O-Acetyl GD3 Ganglioside

Cited by 13 publications

References 62 publications

Regulation of adult neural progenitor cell functions by purinergic signaling

Regulation of adult neural progenitor cell functions by purinergic signaling

GD3 synthase deletion alters retinal structure and impairs visual function in mice

CD60b: Enriching Neural Stem/Progenitor Cells from Rat Development into Adulthood

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Neuroblast Migration and P2Y1Receptor Mediated Calcium Signalling Depend on 9-O-Acetyl GD3 Ganglioside

Cited by 13 publications

References 62 publications

Regulation of adult neural progenitor cell functions by purinergic signaling

Regulation of adult neural progenitor cell functions by purinergic signaling

GD3 synthase deletion alters retinal structure and impairs visual function in mice

CD60b: Enriching Neural Stem/Progenitor Cells from Rat Development into Adulthood

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Neuroblast Migration and P2Y₁Receptor Mediated Calcium Signalling Depend on 9-O-Acetyl GD3 Ganglioside