Neuroblastoma cells can be classified according to glycosphingolipid expression profiles identified by liquid chromatography-tandem mass spectrometry

Kaneko, Tomonori; Okita, Hajime; Nakajima, Hideki; Iijima, Kazutoshi; Ogasawara, Nao; Miyagawa, Yoshitaka; Katagiri, Yohko U.; Nakagawa, Atsuko; Kiyokawa, Nobutaka; Sato, Toshiharu; Fujimoto, Junichiro

doi:10.3892/ijo_00000779

Cited by 5 publications

(1 citation statement)

References 11 publications

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“…O -acetylated GD2 has recently received significant attention as a novel antigen to target GD2-positive cancers. O AcGD2 is highly expressed by GD2-positive tumors such as sarcomas, neuroblastomas, gliomas, and in small cell lung cancer and breast cancer cells [ 59 , 60 ]. GD2 has been considered for more than two decades as a tumor-associated antigen and a highly valuable therapeutic target in these cancers.…”

Section: Expression and Roles Of Cancer-associated Ganglioside In Malignant Propertiesmentioning

confidence: 99%

Cancer-Associated Glycosphingolipids as Tumor Markers and Targets for Cancer Immunotherapy

Groux-Degroote

Delannoy

2021

IJMS

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Aberrant expression of glycosphingolipids is a hallmark of cancer cells and is associated with their malignant properties. Disialylated gangliosides GD2 and GD3 are considered as markers of neuroectoderm origin in tumors, whereas fucosyl-GM1 is expressed in very few normal tissues but overexpressed in a variety of cancers, especially in small cell lung carcinoma. These gangliosides are absent in most normal adult tissues, making them targets of interest in immuno-oncology. Passive and active immunotherapy strategies have been developed, and have shown promising results in clinical trials. In this review, we summarized the current knowledge on GD2, GD3, and fucosyl-GM1 expression in health and cancer, their biosynthesis pathways in the Golgi apparatus, and their biological roles. We described how their overexpression can affect intracellular signaling pathways, increasing the malignant phenotypes of cancer cells, including their metastatic potential and invasiveness. Finally, the different strategies used to target these tumor-associated gangliosides for immunotherapy were discussed, including the use and development of monoclonal antibodies, vaccines, immune system modulators, and immune effector-cell therapy, with a special focus on adoptive cellular therapy with T cells engineered to express chimeric antigen receptors.

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Section: Expression and Roles Of Cancer-associated Ganglioside In Malignant Propertiesmentioning

confidence: 99%

Cancer-Associated Glycosphingolipids as Tumor Markers and Targets for Cancer Immunotherapy

Groux-Degroote

Delannoy

2021

IJMS

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GD2 and its biosynthetic enzyme GD3 synthase promote tumorigenesis in prostate cancer by regulating cancer stem cell behavior

Bhat,

Mohapatra,

Luan

et al. 2024

Sci Rep

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While better management of loco-regional prostate cancer (PC) has greatly improved survival, advanced PC remains a major cause of cancer deaths. Identification of novel targetable pathways that contribute to tumor progression in PC could open new therapeutic options. The di-ganglioside GD2 is a target of FDA-approved antibody therapies in neuroblastoma, but the role of GD2 in PC is unexplored. Here, we show that GD2 is expressed in a small subpopulation of PC cells in a subset of patients and a higher proportion of metastatic tumors. Variable levels of cell surface GD2 expression were seen on many PC cell lines, and the expression was highly upregulated by experimental induction of lineage progression or enzalutamide resistance in CRPC cell models. GD2high cell fraction was enriched upon growth of PC cells as tumorspheres and GD2high fraction was enriched in tumorsphere-forming ability. CRISPR-Cas9 knockout (KO) of the rate-limiting GD2 biosynthetic enzyme GD3 Synthase (GD3S) in GD2high CRPC cell models markedly impaired the in vitro oncogenic traits and growth as bone-implanted xenograft tumors and reduced the cancer stem cell and epithelial-mesenchymal transition marker expression. Our results support the potential role of GD3S and its product GD2 in promoting PC tumorigenesis by maintaining cancer stem cells and suggest the potential for GD2 targeting in advanced PC.

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Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells

Ojima

Shibata

Saito

et al. 2015

Sci Rep

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Glycosphingolipids (GSLs) are glycoconjugates that function as mediators of cell adhesion and modulators of signal transduction. Some well-defined markers of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are glycoconjugates, such as SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. However, Comprehensive GSL profiles of hiPSCs have not yet been elucidated. The global images of GSLs from the parental cells, hiPSCs, and differentiated cells revealed that there are parental cell-independent specific glycolipids, including Globo H (fucosyl-Gb5Cer) and H type1 antigen (fucosyl-Lc4Cer) that are novel markers for undifferentiated hiPSCs. Interestingly, undifferentiated hiPSCs expressed H type 1 antigen, specific for blood type O, regardless of the cells’ genotypes. Thus, in this study, we defined the dynamics of GSL remodeling during reprogramming from parental cell sets to iPSC sets and thence to iPSC-neural cells.

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Neuroblastoma cells can be classified according to glycosphingolipid expression profiles identified by liquid chromatography-tandem mass spectrometry

Cited by 5 publications

References 11 publications

Cancer-Associated Glycosphingolipids as Tumor Markers and Targets for Cancer Immunotherapy

Cancer-Associated Glycosphingolipids as Tumor Markers and Targets for Cancer Immunotherapy

GD2 and its biosynthetic enzyme GD3 synthase promote tumorigenesis in prostate cancer by regulating cancer stem cell behavior

Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells

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