Neurochemical characteristics of calbindin-like immunoreactive coeliac-cranial mesenteric ganglion complex (CCMG) neurons supplying the pre-pyloric region of the porcine stomach

Palus, Katarzyna; Bulc, Michał; Czajkowska, Marta; Miciński, Bartosz; Całka, Jarosław

doi:10.1016/j.tice.2017.12.002

Cited by 6 publications

(7 citation statements)

References 48 publications

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“…This was consistent with the characteristics of OIC stools in previous reports, indicating that the OIC rat model was successfully established (28,29). The functional activity of the GI tract is primarily regulated by the enteric nervous system (ENS), which mainly includes the submucosal plexus between the mucosa and the submucosa and the myenteric plexus located between the circular and longitudinal muscle (14,(41)(42)(43)(44). Enteric nerve cells are divided into intrinsic primary afferent or sensory neurons, intermediate nerve cells and motor nerve cells.…”

Section: Discussionsupporting

confidence: 88%

“…In the present study, MOR was co-expressed with CB and CGRP in the colonic nerve cells. Studies have shown that CB is primarily expressed in the primary sensory afferent neurons and participates in the sensory information transmission along the GI tract (43,(46)(47)(48). CGRP is released by the submucosal plexus cells that transmit mechanical and chemical irritative sensory information along the GI tract (46,49,50).…”

Section: Discussionmentioning

confidence: 99%

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P2Y1 receptor in the colonic submucosa of rats and its association with opioid‑induced constipation

Zhao

Ren

et al. 2022

Exp Ther Med

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The aim of the present study was to explore the expression changes of P2Y purinergic receptor 1 (P2Y1) in the distal colonic submucosa of opioid-induced constipation (OIC) rats and its association with the occurrence of OIC, an OIC rat model was generated by intraperitoneal injection of loperamide hydrochloride, a selective agonist of µ-opioid receptors (MORs). At 7 days post-treatment, the model was assessed by analyzing stool scores and calculating the gastrointestinal (GI) transit ratio of rats. The distribution of P2Y1-expressing neurons in the colonic submucosal plexus was demonstrated by immunofluorescence (IF). Western blotting was performed to evaluate the expression changes of MOR, P2Y1 and ATP synthase subunit β (ATPB) proteins in the colonic submucosa, while reverse transcription-quantitative PCR (RT-qPCR) analysis was performed to determine the relative mRNA expression of MOR and P2Y1. After 7 days, the feces of OIC rats exhibited an appearance of sausage-shaped pieces and both the stool weight and GI transit ratio of OIC rats were significantly decreased. IF revealed co-expression of P2Y1 and calbindin and MOR and ATPB in the nerve cells of the distal colonic submucosal plexus. Moreover, RT-qPCR analysis showed that the MOR mRNA levels were significantly increased in the distal colonic submucosa of OIC rats, while mRNA levels of P2Y1 were decreased. WB showed that in the distal colonic submucosa of OIC rats, MOR protein expression was increased, whereas that of P2Y1 was significantly decreased. GI transit ratio analysis suggested that the P2Y agonist ATP significantly relieved constipation symptoms in rats, while the P2Y inhibitor MRS2179 aggravated these symptoms. Finally, P2Y1 expression change was shown to be associated with the occurrence of OIC, while expression of MOR and P2Y1 was associated with OIC development in rats.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

P2Y1 receptor in the colonic submucosa of rats and its association with opioid‑induced constipation

Zhao

Ren

et al. 2022

Exp Ther Med

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“…Some PG neurons express the calcium‐binding protein calbindin (CB). In the CG/SMG of rats and pigs, the percentage of CB‐IR neurons is approximately 10% (Masliukov, Konovalov, et al, 2012; Masliukov, Korobkin, et al, 2012; Palus et al, 2018), but only a few CB‐IR neurons have been observed in cats and guinea pigs (Kuramoto et al, 1990; Masliukov, Konovalov, et al, 2012; Masliukov, Korobkin, et al, 2012). In the porcine IMG, one‐third of the neurons are CB‐IR neurons (Palus et al, 2018).…”

Section: Anatomy Of the Sympathetic Innervation Of The Gastrointestin...mentioning

confidence: 99%

“…In the pig, of the neurons innervating the stomach, 77%–94% were TH‐IR, 40%–47% were NPY‐IR, 12%–14% were SOM‐IR, 7%–8% were GAL‐IR, and 8% were CB‐IR. SOM‐IR neurons innervating the prepyloric area of the porcine stomach were located in the CG (Palus et al, 2017, 2018; Palus & Całka, 2016; Zalecki, 2012). TH‐IR and NPY‐IR sympathetic fibers innervate the ganglia of the myenteric and submucosal plexuses, blood vessels, both muscular layers (circular and longitudinal), and the muscularis mucosae.…”

Section: Anatomy Of the Sympathetic Innervation Of The Gastrointestin...mentioning

confidence: 99%

“…In the pig, of the neurons innervating the stomach, 77%-94% were TH-IR, 40%-47% were NPY-IR, 12%-14% were SOM-IR, 7%-8% were GAL-IR, and 8% were CB-IR. SOM-IR neurons innervating the prepyloric area of the porcine stomach were located in the CG (Palus et al, 2017(Palus et al, , 2018Palus & Całka, 2016;Zalecki, 2012).…”

Section: Connections Of Sympathetic Pg Neurons With Different Parts O...mentioning

confidence: 99%

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Sympathetic innervation of the development, maturity, and aging of the gastrointestinal tract

Maslyukov

Emanuilov

Budnik

2022

The Anatomical Record

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The sympathetic nervous system inhibits gut motility, secretion, and blood flow in the gut microvasculature and can modulate gastrointestinal inflammation. Sympathetic neurons signal via catecholamines, neuropeptides, and gas mediators. In the current review, we summarize the current understanding of the mature sympathetic innervation of the gastrointestinal tract with a focus mainly on the prevertebral sympathetic ganglia as the main output to the gut. We also highlight recent work regarding the developmental processes of sympathetic innervation. The anatomy, neurochemistry, and connections of the sympathetic prevertebral ganglia with different parts of the gut are considered in adult organisms during prenatal and postnatal development and aging. The processes and mechanisms that control the development of sympathetic neurons, including their migratory pathways, neuronal differentiation, and aging, are reviewed.

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Immunohistochemical relationships of huntingtin-associated protein 1 with enteroendocrine cells in the pyloric mucosa of the rat stomach

et al. 2020

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Neurochemical characteristics of calbindin-like immunoreactive coeliac-cranial mesenteric ganglion complex (CCMG) neurons supplying the pre-pyloric region of the porcine stomach

Cited by 6 publications

References 48 publications

P2Y1 receptor in the colonic submucosa of rats and its association with opioid‑induced constipation

P2Y1 receptor in the colonic submucosa of rats and its association with opioid‑induced constipation

Sympathetic innervation of the development, maturity, and aging of the gastrointestinal tract

Immunohistochemical relationships of huntingtin-associated protein 1 with enteroendocrine cells in the pyloric mucosa of the rat stomach

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