Neurochemical profile of the selective and silent 5-HT1A receptor antagonist WAY100135: an in vivo microdialysis study

5-HT1D Receptors Regulate 5-HT Release in the Rat Raphe Nuclei

“…6A) and (+)WA Y100135 (Bjork et al 1991;Fletcher et al 1993;Routledge et al 1993); and (2) the 5-HT1A agonist 8-OH-DPAT inhibited tritium release (Fig. 4).These observations are consistent with the suppressant effect of microiontophoretic application of 8-OH-DPAT on the ii.ring activity of 5-HT neurons in the dorsal raphe (Blier et al 1987).…”

“…In the pres- The effect of 5-CT (100 nM) was also blocked by the 5-HT1A antagonists S-UH-301 ( Fig. 6A; Bjork et al 1991) and ( + )WAY100135 Routledge et al 1993). Observed S2/S1 ratios were 0.08 ± 0.03 for 5-CT (100 nM, n = 10) and 0.74 ± 0.06 for 5-CT (100 nM) plus ( + )WAY100135 (1 µM; n = 3), respectively (p < .001).…”

Corticosteroids Alter the 5-HT1A Receptor-Mediated Response in CA1 Hippocampal Pyramidal Cells

“…A higher concentration of 5-HT was required to give the same magnitude of hyperpolarization in a cell from a HCT treated animal as compared to a SHAM treated animal. Microdialysis has shown that the physiological concentration of 5-HT seen at the synapse is about 1 M (Kreiss and Lucki 1994;Kreiss et al 1995;Routledge et al 1993). The magnitude of hyperpolarization elicited by low micromolar concentrations of 5-HT in a cell from a HCT treated animal would be smaller than that seen in a cell from a SHAM treated rat.…”

NMDA‐induced glutamate and aspartate release from rat cortical pyramidal neurones: evidence for modulation by a 5‐HT1A antagonist

“…This is an antagonist in both pre and post-synaptic 5-HTlA models and is devoid of agonist properties up to 10 mg kg-'. (Fletcher et al, 1993;Routledge et al, 1993). Since the activity of WAY 100135 resides in the (+ )-enantiomer, (+ )-WAY 100135 was used for all experiments.…”