Neuroendocrine Cells along the Digestive Tract Express Neuropilin-2

Cohen, Tzafra; Gluzman‐Poltorak, Zoya; Brodzky, Asia; Meytal, Vered; Sabo, Edmond; Misselevich, Innes; Boss, J. H.; Resnick, Murray B.; Shneyvas, Dova; Eldar, Samuel; Neufeld, Gera

doi:10.1006/bbrc.2001.4958

Cited by 31 publications

(23 citation statements)

References 43 publications

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“…NRP2 expression on tumor cells has been correlated with tumor progression and prognosis, albeit in a cancer-specific manner. In bladder cancer and osteosarcoma, NRP2 expression was found to be correlated with tumor progression [17,24]; however, the loss of NRP2 expression in gastrointestinal carcinoid tumors was found to be correlated with tumor progression [25]. In melanoma, NRP2 has been implicated in melanoma-endothelial cell interaction, with preliminary studies showing inhibition of cell proliferation due to NRP2 knockdown and neutralizing antibody inhibition [7].…”

Section: Discussionmentioning

confidence: 99%

Neuropilin-2 promotes melanoma growth and progression in vivo

et al. 2016

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Tumor cell interactions with their microenvironment, and neighboring endothelial cells in particular, are critical for tumor cell survival and the metastatic process. Within the spectrum of tumors, melanomas are notorious for their ability to metastasize at a relatively early stage of development; however, little is known about the molecular pathways mediating this process. We recently performed a screen to assess critical mediators of melanoma metastasis by evaluating melanoma-endothelial cell communication. Neuropilin-2 (NRP2), a cell surface receptor involved in angiogenesis and axonal guidance, was found to be an important mediator of melanoma-endothelial cell cross-talk in these studies. Here we seek to further define the role of NRP2 in melanoma growth and progression. We use stable gene silencing of NRP2 in melanomas from varying stages of tumor progression to define the role of NRP2 in melanoma growth, migration, invasion, and metastasis. We found that NRP2 gene silencing in metastatic melanoma cell lines inhibited tumor cell growth in vitro; furthermore, knockdown of NRP2 expression in the metastatic melanoma cell line 1205Lu significantly inhibited in-vivo tumor growth and metastasis. We conclude that NRP2 plays an important role in mediating melanoma growth and metastasis and suggest that targeting this cell surface molecule may represent a significant therapeutic strategy for patients diagnosed with aggressive forms of melanoma.

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Section: Discussionmentioning

confidence: 99%

Neuropilin-2 promotes melanoma growth and progression in vivo

et al. 2016

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“…Interestingly, in patients in which the tumor cells coexpressed NRP-1 and NRP-2 mRNA, the prognosis was worse. Also of interest is a study in gastrointestinal carcinoid tumors in which loss of NRP-2 expression correlated with tumor progression (69). Because NRP-2 is a receptor for SEMA3F, it is possible that the loss of SEMA3F activity contributes to malignant progression in these tumors, however, further study is clearly required to establish such a link.…”

Section: Role Of Neuropilins Ontumor Cells Expressionmentioning

confidence: 99%

The role of neuropilins in cancer

Ellis

2006

Molecular Cancer Therapeutics

232

194

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Neuropilins are multifunctional non -tyrosine kinase receptors that bind to class 3 semaphorins and vascular endothelial growth factor. NRP-1 and NRP-2 were first identified for their key role in mediating axonal guidance in the developing nervous system through their interactions with class 3 semaphorins. Growing evidence supports a critical role for these receptors in tumor progression. Neuropilin expression is up-regulated in multiple tumor types, and correlates with tumor progression and prognosis in specific tumors. Neuropilins may indirectly mediate effects on tumor progression by affecting angiogenesis or directly through effects on tumor cells. This article reviews emerging evidence for the role of neuropilins in tumor biology. The therapeutic implications of these data are far-reaching and suggest that neuropilin-targeted interventions may be useful as a component of antineoplastic therapy.

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“…In situ hybridization was performed essentially as described [30] with the following modifications: sense and antisense cRNA probes were synthesized using T7 RNA polymerase. Tissues were fixed in formalin and embedded in paraffin.…”

Section: In Situ Hybridizationmentioning

confidence: 99%