Neuroendocrine cells and nerves of the lung

Adriaensen, Dirk; Scheuermann, D.W.

doi:10.1002/ar.1092360111

Cited by 122 publications

(103 citation statements)

References 127 publications

(127 reference statements)

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“…CGRP and PGP 9.5 positive NEBs were found throughout all the later developmental stages examined in the present study. Expression of PGP 9.5 in the NEBs has been demonstrated previously in adult mammals including the rat [1,17,26]. However, the or developing airway of hamster [22], rabbit [4] cat and pig of CGRP, PGP 9.5, Serotonin and Chromogranin A in Rat Respiratory Tract 369 [13].…”

Section: Discussionmentioning

confidence: 78%

“…Wistar rat fetuses from embryonic days (ED) 15,17,19,20,21 and neonatal rats from postnatal days (PD) 0, 1,3,7,11,14,20,21,30, 60 were investigated. Four animals were obtained from each developmental stage and the day of conception was defined as embryonic day 0.…”

Section: Methodsmentioning

confidence: 99%

“…PGP 9.5 is a cytoplasmic neuron-specific protein that is used as a general marker of systemic neurons [6,17,28]. Previous studies have shown that PGP 9.5 is distributed in the respiratory system [1,17,26]. However, these studies were restricted to investigating the adult animal model.…”

mentioning

confidence: 99%

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Change in Distribution and Density of CGRP, PGP 9.5, Serotonin and Chromogranin A Immunoreactivity in the Perinatal Rat Respiratory Tract.

Inoue

Fujimiya

Maeda

et al. 1997

Acta Histochem. Cytochem

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Section: Discussionmentioning

confidence: 78%

Section: Methodsmentioning

confidence: 99%

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Change in Distribution and Density of CGRP, PGP 9.5, Serotonin and Chromogranin A Immunoreactivity in the Perinatal Rat Respiratory Tract.

Inoue

Fujimiya

Maeda

et al. 1997

Acta Histochem. Cytochem

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“…Therefore, our present study is the first to document precisely the developmental course of pulmonary endocrine cells in sheep lungs by the immunohistochemical quantitative analysis. Recent immunohistochemical studies of various kind of mammals showed that the pulmonary endocrine cells have various kinds of neuroendocrine markers such as serotonin [1,9,11,12,18,21,28], bombesin [1,6,12,23,24,28], gastrin-releasing peptide [1], cholecystokinin [27], calcitonin [1,12,23], calcitonin gene related peptide [1,28], leuenkephalin [1,12], NSE [1,8,18] and chromogranin [1].…”

Section: Resultsmentioning

confidence: 99%

“…Pulmonary endocrine cells are widely distributed throughout the airway mucosa and are thought to play an important role in lung development and maturation from intrauterine life to the postnatal period [1,6,8,9,11,16,18,27]. The principal role of pulmonary endocrine cells during intrauterine life is considered to involve the maintenance of active pulmonary vasoconstriction in the relatively hypoxic lung, as well as the rapid vasodilation upon the aeration of the lung at birth [12].…”

mentioning

confidence: 99%

Quantitative Study of Pulmonary Endocrine Cells in Fetal, Postnatal and Adult Sheep

Asabe

Jennings²,

Harrison³

et al. 2004

J. Vet. Med. Sci.

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ABSTRACT. Using light microscopic immunohistochemistry, neuron-specific enolase (NSE)-positive endocrine cells were quantitatively analyzed in the sheep lung during different stages of development from the canalicular stages to adulthood. In all stages, NSE-positive endocrine cells were usually located in the bronchi and bronchioles as solitary cells, although a few NSE-positive cell clusters, the socalled neuroepithelial bodies, were found in some places. The number of NSE-positive endocrine cells decreased with advanced stages of gestation. In the late alveolar stage, the number of NSE-positive endocrine cells reached its bottom during the fetal period. There was a gradual upturn after birth. The overall pattern of growth and differentiation of the endocrine cells is most likely species-related and depends on the state of airway development; the number of the endocrine cells of almost all animals, excluding the sheep, in relation to the size of the lung reaches a peak in the late fetal and early neonatal periods and decreases shortly thereafter. NSE-posit ive endocrine cells were also predominantly located in the large airways during the early stage of development (canalicular stage), and were found more frequently in the small peripheral airways towards the term. These results show the number of NSE-positive endocrine cells in t he sheep to be different from that seen in other species. KEY WORDS: endocrine cell, lung, sheep.

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Immunocytochemical localization of O2-sensing protein (NADPH oxidase) in chemoreceptor cells

Youngson

Nurse

Yeger

et al. 1997

Microsc. Res. Tech.

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A potential candidate for an oxygen‐sensing protein in chemoreceptor cells is a heme‐linked multicomponent NADPH oxidase, originally described in neutrophils. The postulated function for the oxidase in chemoreceptor cells is to signal changes in oxygen levels (either in the blood or in the airway lumen) via changes in oxygen metabolite production. An alteration in either superoxide (or dismuted hydrogen peroxide) production may affect the gating properties of the O2‐sensitive K+ channels. We have previously reported immunohistochemical localization of gp91 glycoprotein component of the oxidase to the plasma membrane of pulmonary neuroepithelial body (NEB) cells. In this study we have investigated the immunocytochemical localization of the other polypeptide components of the oxidase in NEB cells and in the glomus cells of the carotid body. Cultures of dissociated fetal rabbit NEB cells and newborn rat glomus cells were immunostained with specific antibodies recognizing the various polypeptide subunits of the oxidase using indirect immunofluorescence methods. Immunostaining with the anti‐oxidase antibodies revealed strong positive reaction in both NEB and glomus cell clusters while other cells were unstained. The positive reaction product was localized to the plasma membrane and/or cytoplasm and no nuclear staining was observed. Live cell labelling studies with anti‐p22 antibody showed positive immunofluorescence on the surface of NEB cells, suggesting that this component of the oxidase is also associated with the plasma membrane. In glomus cells, similar strongly positive immunofluorescence signal was observed for p22 and gp91 in paraformaldehyde‐fixed cultures, regardless whether they were permeabilized or not. Taken together, our findings of cell surface localization of gp91 and p22 components of the oxidase in chemoreceptive cells suggests that the heme‐linked cytochrome b558 component is associated with the plasma membrane. This association allows for direct interaction with the O2‐sensitive K+ channel thus forming the molecular complex of membrane bound O2 sensor. Microsc. Res. Tech. 37:101–106, 1997. © 1997 Wiley‐Liss, Inc.

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Neuroendocrine cells and nerves of the lung

Cited by 122 publications

References 127 publications

Change in Distribution and Density of CGRP, PGP 9.5, Serotonin and Chromogranin A Immunoreactivity in the Perinatal Rat Respiratory Tract.

Change in Distribution and Density of CGRP, PGP 9.5, Serotonin and Chromogranin A Immunoreactivity in the Perinatal Rat Respiratory Tract.

Quantitative Study of Pulmonary Endocrine Cells in Fetal, Postnatal and Adult Sheep

Immunocytochemical localization of O2-sensing protein (NADPH oxidase) in chemoreceptor cells

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