Neuroendocrine cells are present in the domestic fowl ovary

Salcedo, Pablo Hofmann; Saldaña, Armida Báez; Fortoul, Teresa I.; Pliego, Margarita González del; Ospina, Gabriel Gutiérrez

doi:10.1111/joa.12002

Cited by 9 publications

(6 citation statements)

References 30 publications

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“…The sympathetic fibers predominantly release noradrenaline (NA) and vasoactive intestinal peptide (VIP), and the sensory fibers release peptides such as CGRP, substance P (SP) and galanin, among others [9]- [12]. Under physiological conditions, nerve fibers are involved in early follicular development and ovarian steroidogenesis [2] [13]- [15], i.e., NA stimulates androgen and progesterone secretion [16] [17], VIP stimulates estrogen production [18] [19] and CGRP and SP are involved in ovarian vascular fluid [9] [12]. Ovarian intrinsic neurons have been studied in several mammals (rats, monkeys and humans) [5] [7] and birds (hen and emu) [18] [19] and modulate steroidogenesis and local vascular fluid [2] [6] [14].…”

Section: Introductionmentioning

confidence: 99%

“…Under physiological conditions, nerve fibers are involved in early follicular development and ovarian steroidogenesis [2] [13]- [15], i.e., NA stimulates androgen and progesterone secretion [16] [17], VIP stimulates estrogen production [18] [19] and CGRP and SP are involved in ovarian vascular fluid [9] [12]. Ovarian intrinsic neurons have been studied in several mammals (rats, monkeys and humans) [5] [7] and birds (hen and emu) [18] [19] and modulate steroidogenesis and local vascular fluid [2] [6] [14]. In the rat ovary, intrinsic neurons are located in the hilum and medulla of the ovary, first appear in the cortex during the juvenile period [5], and increase significantly during prepubertal development, reaching maximum values before puberty [6].…”

Section: Introductionmentioning

confidence: 99%

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Morphology and Chemical Phenotype of the Ovarian Intrinsic Neurons in Neonate and Sexually Mature Reproductive Guinea Pig

Luna¹,

Barrientos²,

Alatriste³

et al. 2015

ARSci

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Introduction: The existence of ovarian intrinsic neurons is well established. However, the morphology and chemical phenotype are not completely characterized and are even unknown for some species used in medical research. The purpose of this work was to determine the morphology and chemical phenotype of intrinsic neurons of the guinea pig ovary at two ages: neonates (0 days old) and sexually mature reproductive animals (90 days old). Materials and Methods: For the morphological analysis, we employed the modified Golgi-Cox impregnation technique. For the chemical phenotype, we used immunohistochemistry and the following antibodies; tyrosine hydroxylase (TH), calcitonin gene-related peptide (CGRP), transient receptor potential type 1 (TRPV1), neuron-specific nuclear protein (NeuN) and proto-oncogene product of the cFos gene (cFos). We also used enzyme histochemistry for NADPH-diaphorase detection. Results: The number of intrinsic neurons in the neonate ovary was low in comparison to the adult guinea pig ovary. The intrinsic neurons were located in the cortex and the ovarian medulla; some were isolated or clustered, forming ganglia, and others were interconnected and formed networks. The neurons were small, medium or large. In the cortex of neonate vs adult ovaries, the small and medium neurons comprised 23% vs 36% and 5.2% vs 11.6%, respectively. In the medulla, the percent of the same neurons was 10.1% vs 10.1% and 1.1% vs 2.2% in the neonate and adult, respectively. In both cortex and medulla < 1% were large neurons at two ages. Also, the neurons were rounded, fusiform or multipolar. In the cortex, they were 12.7% vs 20.9%, 14.9% vs 24.2% and 1.1% vs 3.0%, respectively. In the medulla, the percent of small vs medium neurons was 6% vs 7.1% and 4.1% vs 4.8% in the neonate and adult ovary, respectively, and <1% were large neurons at both ages. The chemical phenotypes were in the neonate and adult: TH/NeuN-positive neurons, 16.3% vs 26.5%; CGRP/NeuN, 13.5% vs 35.8%; TRPV1/NeuN, 10.2% vs 38.6%; and cFos/NeuN, 4.6% vs 5.4%, re-* Corresponding author. F. Luna et al. 14 spectively. The percent of NADPHd-positive cells in the cortex was 9.5% vs 25.1% and 3.2% vs 62.2% in the medulla in the neonate and adult, respectively. Conclusion: Altogether, these data showed that the number of ovarian intrinsic neurons was low at birth and increased in the sexually mature reproductive guinea pig. The chemical phenotype was rich and peptidergic, catecholaminergic and nitrergic in nature and positive for cFos immunoreactivity. Therefore, intrinsic neurons can be chemical sensors inside of the gonad and transmit signal to the central nervous system.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Morphology and Chemical Phenotype of the Ovarian Intrinsic Neurons in Neonate and Sexually Mature Reproductive Guinea Pig

Luna¹,

Barrientos²,

Alatriste³

et al. 2015

ARSci

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“…Antibodies against cytokeratin 8/18 (KRT 8/18), cytokeratin 5 (KRT 5), and chromogranin A (CHGA) were used to identify luminal[21, 23] (KRT 8/18+, KRT 5−, CHGA−), intermediate[21, 23] (KRT 8/18+, KRT 5+, CHGA−), and basal[21, 23] (KRT 8/18−, KRT5−, CHGA−) epithelial cells. Neuroendocrine cells[21, 24, 25] (KRT 8/18−, KRT 5−, CHGA+) were also identified with this stain combination. Antibodies against smooth muscle actin (ACTA2), protein tyrosine phosphatase receptor type C (PTPRC, also known as CD45),and vimentin (VIM) were used to identify fibrocytes[21, 26–30] (ACTA2+, PTPRC+, VIM+), and other hematolymphoid cells (PTPRC+, not a fibrocyte).…”

Section: Methodsmentioning

confidence: 85%

“…Antibodies against cytokeratin 8/18 (KRT 8/18), cytokeratin 5 (KRT 5), and chromogranin A (CHGA) are used to identify luminal[21, 23] (KRT 8/18+, KRT 5−, CHGA−, Figure 1B), intermediate[21, 23] (KRT 8/18+, KRT 5+, CHGA−, Figure 1C), and basal[21, 23] (KRT 8/18−, KRT 5−, CHGA−, Figure 1D) epithelial cells. Neuroendocrine cells[21, 24, 25] (KRT 8/18−, KRT 5−, CHGA+, Figure 1E) are also identified. Epithelial cells in general and neuroendocrine cells in particular are not detected in the capsule (Figure 1F) but are present in the peripheral region (Figure 1G), the periurethral region (Figure 1H) and the prostatic urethra (Figure 1I).…”

Section: Resultsmentioning

confidence: 99%

An immunohistochemical prostate cell identification key indicates that aging shifts procollagen 1A1 production from myofibroblasts to fibroblasts in dogs prone to prostate-related urinary dysfunction

Ruetten

Cole

Wehber

et al. 2020

Preprint

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34Background: The identity and spatial distribution of prostatic cell types has been 35 determined in humans but not in dogs, even though aging-and prostate-related voiding 36 disorders are common in both species and mechanistic factors, such as prostatic collagen 37 accumulation, appear to be shared between species. In this publication we characterize 38 the regional distribution of prostatic cell types in the young intact dog to enable 39 comparisons with human and mice and we examine how the cellular source of 40 procollagen 1A1 changes with age in intact male dogs. 41Methods: A multichotomous decision tree involving sequential immunohistochemical 42 stains was validated for use in dog and used to identify specific prostatic cell types and 43 determine their distribution in the capsule, peripheral, periurethral and urethral regions of 44 the young intact canine prostate. Prostatic cells identified using this technique include 45 perivascular smooth muscle cells, pericytes, endothelial cells, luminal, intermediate, and 46 basal epithelial cells, neuroendocrine cells, myofibroblasts, fibroblasts, fibrocytes, and 47 other hematolymphoid cells. Corresponding images were made freely accessible through 48 the GUDMAP database at https://doi.org/10.25548/16-WMM4. 49 Results: The prostatic peripheral region harbors the largest proportion of epithelial cells. 50 Aging does not change the density of hematolymphoid cells, fibroblasts, and 51 myofibroblasts in the peripheral region or in the fibromuscular capsule, regions where we 52 previously observed aging-and androgen-mediated increases in prostatic collagen 53 abundance Instead, we observed aging-related changes the procollagen 1A1 positive 54 prostatic cell identity from a myofibroblast to a fibroblast. 55 Conclusions: Hematolymphoid cells and myofibroblasts are often identified as sources 56 of collagen in tissues prone to aging-related fibrosis. We show that these are not the likely 57 sources of pathological collagen synthesis in older intact male dogs. Instead, we identify 58 an aging-related shift in the prostatic cell type producing procollagen 1A1 that will help 59 direct development of cell type and prostate appropriate therapeutics for collagen 60 accumulation. 61 62 Keywords: fibrosis, BPH, Collagen, LUTS 63 64 1. Introduction 65 Men and dogs develop prostatic disorders spontaneously and in an aging-and 66 androgen-dependent fashion.[1-11] Human and dog prostates organize into distinct 67 anatomical zones with an externally bound fibromuscular capsule (anatomical features 68 not shared by the mouse prostate).[12] Careful histological and molecular analyses of 69 human prostate have revolutionized our understanding of the cell types of the prostate, 70revealing a gland that contains at least five epithelial cell types and three fibroblast-like 71 stromal cell types that are organized into three zones. [13, 14] Canine prostate cell types 72 and their spatial distribution across the gland have not been extensively examined using 73 modern molecular a...

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“…Antibodies against cytokeratin 8/18 (KRT 8/18), cytokeratin 5 (KRT 5), and chromogranin A (CHGA) were used to identify luminal [ 21 , 23 ] (KRT 8/18+, KRT 5-, CHGA-), intermediate [ 21 , 23 ] (KRT 8/18+, KRT 5+, CHGA-), and basal [ 21 , 23 ] (KRT 8/18-, KRT 5-, CHGA-) epithelial cells. Neuroendocrine cells [ 21 , 24 , 25 ] (KRT 8/18-, KRT 5-, CHGA+) were also identified with this stain combination. Antibodies against smooth muscle actin (ACTA2), protein tyrosine phosphatase receptor type C (PTPRC, also known as CD45), and vimentin (VIM) were used to identify fibrocytes [ 21 , 26 – 30 ] (ACTA2+, PTPRC+, VIM+), and other hematolymphoid cells (PTPRC+, not a fibrocyte).…”

Section: Methodsmentioning

confidence: 85%

An immunohistochemical prostate cell identification key indicates that aging shifts procollagen 1A1 production from myofibroblasts to fibroblasts in dogs prone to prostate-related urinary dysfunction

et al. 2020

View full text Add to dashboard Cite

Background The identity and spatial distribution of prostatic cell types has been determined in humans but not in dogs, even though aging- and prostate-related voiding disorders are common in both species and mechanistic factors, such as prostatic collagen accumulation, appear to be shared between species. In this publication we characterize the regional distribution of prostatic cell types in the young intact dog to enable comparisons with human and mice and we examine how the cellular source of procollagen 1A1 changes with age in intact male dogs. Methods A multichotomous decision tree involving sequential immunohistochemical stains was validated for use in dog and used to identify specific prostatic cell types and determine their distribution in the capsule, peripheral, periurethral and urethral regions of the young intact canine prostate. Prostatic cells identified using this technique include perivascular smooth muscle cells, pericytes, endothelial cells, luminal, intermediate, and basal epithelial cells, neuroendocrine cells, myofibroblasts, fibroblasts, fibrocytes, and other hematolymphoid cells. To enhance rigor and transparency, all high resolution images (representative images shown in the figures and biological replicates) are available through the GUDMAP database at https://doi.org/10.25548/16-WMM4 . Results The prostatic peripheral region harbors the largest proportion of epithelial cells. Aging does not change the density of hematolymphoid cells, fibroblasts, and myofibroblasts in the peripheral region or in the fibromuscular capsule, regions where we previously observed aging- and androgen-mediated increases in prostatic collagen abundance Instead, we observed aging-related changes the procollagen 1A1 positive prostatic cell identity from a myofibroblast to a fibroblast. Conclusions Hematolymphoid cells and myofibroblasts are often identified as sources of collagen in tissues prone to aging-related fibrosis. We show that these are not the likely sources of pathological collagen synthesis in older intact male dogs. Instead, we identify an aging-related shift in the prostatic cell type producing procollagen 1A1 that will help direct development of cell type and prostate appropriate therapeutics for collagen accumulation.

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Neuroendocrine cells are present in the domestic fowl ovary

Cited by 9 publications

References 30 publications

Morphology and Chemical Phenotype of the Ovarian Intrinsic Neurons in Neonate and Sexually Mature Reproductive Guinea Pig

Morphology and Chemical Phenotype of the Ovarian Intrinsic Neurons in Neonate and Sexually Mature Reproductive Guinea Pig

An immunohistochemical prostate cell identification key indicates that aging shifts procollagen 1A1 production from myofibroblasts to fibroblasts in dogs prone to prostate-related urinary dysfunction

An immunohistochemical prostate cell identification key indicates that aging shifts procollagen 1A1 production from myofibroblasts to fibroblasts in dogs prone to prostate-related urinary dysfunction

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