Neurogenic differentiation capacity of subacromial bursal tissue—derived stem cells

Aydın, Adem; Duruksu, Gökhan; Erman, Gülay; Subaşı, Cansu; Aksoy, Aziz; Ünal, Zehra Seda; Karaöz, Erdal

doi:10.1002/jor.22484

Cited by 11 publications

(7 citation statements)

References 26 publications

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“…2 ). Interestingly, BS cells have also been shown to be able to undergo neurogenic [ 54 ] and tendogenic differentiation [ 55 ] underlining their potential impact for the treatment of subacromial pathology. Because of the similarities between both cell types, as shown in this work, it is also conceivable that BMSCs, which can be easily obtained from bone marrow, might be harnessed to augment reconstitution of the subacromial bursa after surgical removal, providing potential support for the restoration of the gliding mechanism after such treatments.…”

Section: Discussionmentioning

confidence: 99%

Characterization of bursa subacromialis-derived mesenchymal stem cells

et al. 2015

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IntroductionThe bursa subacromialis (BS) provides the gliding mechanism of the shoulder and regenerates itself after surgical removal. Therefore, we explored the presence of mesenchymal stem cells (MSCs) within the human adult BS tissue and characterized the BS cells compared to MSCs from bone marrow (BMSCs) on a molecular level.MethodsBS cells were isolated by collagenase digest from BS tissues derived from patients with degenerative rotator cuff tears, and BMSCs were recovered by adherent culture from bone-marrow of patients with osteoarthritis of the hip. BS cells and BMSCs were compared upon their potential to proliferate and differentiate along chondrogenic, osteogenic and adipogenic lineages under specific culture conditions. Expression profiles of markers associated with mesenchymal phenotypes were comparatively evaluated by flow cytometry, immunohistochemistry, and whole genome array analyses.ResultsBS cells and BMSCs appeared mainly fibroblastic and revealed almost similar surface antigen expression profiles, which was CD44+, CD73+, CD90+, CD105+, CD106+, STRO-1+, CD14−, CD31−, CD34−, CD45−, CD144−. Array analyses revealed 1969 genes upregulated and 1184 genes downregulated in BS cells vs. BMSCs, indicating a high level of transcriptome similarity. After 3 weeks of differentiation culture, BS cells and BMSCs showed a similar strong chondrogenic, adipogenic and osteogenic potential, as shown by histological, immunohistochemical and RT-PCR analyses in contrast to the respective negative controls.ConclusionsOur in vitro characterizations show that BS cells fulfill all characteristics of mesenchymal stem cells, and therefore merit further attention for the development of improved therapies for various shoulder pathologies.

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Section: Discussionmentioning

confidence: 99%

Characterization of bursa subacromialis-derived mesenchymal stem cells

et al. 2015

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“…Although donor variability could not be excluded in previous work, such types of analyses revealed that alkaline phosphatase and cluster of differentiation 34 and 106 expression could further distinguish between BCs and BMCs, and that certain aspects such neurogenic, gland, and vascular development were more present in BCs on array screening for whole genome expression. 9 This is supported by the work of Aydın and coworkers, 10 who found higher levels of neurotrophic factors in BCs compared with BMCs, which might be harnessed for any approach toward restoration of the neuromuscular unit and the neurogenic control of cell turnover of the soft tissues in the subacromial space.…”

Section: See Related Article On Page 2978mentioning

confidence: 80%

Editorial Commentary: Subacromial Bursa—Friend or Foe Within The Shoulder? An Old Debate With New Insights

Steinert

Gohlke

2019

Arthroscopy: The Journal of Arthroscopic & Related Surgery

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There is an ongoing debate about whether to resect or preserve the subacromial bursa during surgical treatment of rotator cuff tears. Neer was the first to systematically describe bursitis as a component of subacromial impingement syndrome that may extend to rotator cuff disease, often discussed as a point of controversy with Uhthoff who first identified the bursa as a contributor to rotator cuff healing, both experimentally and clinically. Because the subacromial bursa provides the gliding mechanism of the shoulder and regenerates itself after surgical removal, interest evolved on the role of the bursa in the healing of rotator cuff tears for evolution of regenerative therapies as a support of arthroscopic repair techniques. In vitro work could identify human subacromial bursa as a source of mesenchymal stem cells, which revealed lineage-specific differentiation capacity, including the tendon and a marker profile that was highly similar to, although in some aspects distinct from, marrow-derived mesenchymal stem cells. Only recently, this knowledge was used in controlled experimental work in vivo to demonstrate superior engraftment of bursal cells within tendon tissue. These findings shed new light on the biology of the subacromial space and provides novel prospects for the clinical use of local stem cells in rotator cuff repair.

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“…3g ). As an effective survival factor, CNTF can reduce tissue destruction during inflammation and protect damaged neurons from degeneration and necrosis [ 66 ]. This explains why the density of myelinated nerve fibers in intermittent dASCs and sustaining dASCs 7d groups were significantly greater than those in the hollow and uASCs groups at both the early stage (6 weeks after cell transplantation) and late stage (12 weeks after cell transplantation), but especially at the early stage (Fig.…”

Section: Discussionmentioning

confidence: 99%

Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function

et al. 2018

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BackgroundPeripheral nerve injury (PNI) is a worldwide issue associated with severe social and economic burden. Autologous nerve grafting, the gold standard treatment for peripheral nerve defects, still has a number of technical limitations. Tissue engineering technology is a novel therapeutic strategy, and mesenchymal stromal cells (MSCs) are promising seed cells for nerve tissue engineering. However, the efficiency of traditional methods for inducing the differentiation of MSCs to Schwann cell-like cells (SCLCs) remains unsatisfactory.MethodsHere, we propose an intermittent induction method with alternate use of complete and incomplete induction medium to induce differentiation of adipose-derived stem cells (ASCs) to SCLCs. The time dependence of traditional induction methods and the efficiency of the intermittent induction method and traditional induction methods were evaluated and compared using immunocytochemistry, quantitative reverse transcription polymerase chain reaction (qRT-PCR), enzyme-linked immunosorbent assay (ELISA), and co-culture with the dorsal root ganglion (DRG) in vitro. Cell transplantation was used to compare the effects of the traditional induction method and the intermittent induction method in repairing sciatic nerve defects in vivo.ResultsThe results of the present study indicated that the intermittent induction method is more efficient than traditional methods for inducing ASCs to differentiate into SCLCs. In addition, SCLCs induced by this method were closer to mature myelinating Schwann cells and were capable of secreting neurotrophins and promoting DRG axon regeneration in vitro. Furthermore, SCLCs induced by the intermittent induction method could repair sciatic nerve defects in rats by cell transplantation in vivo more effectively than those produced by traditional methods.ConclusionIntermittent induction represents a novel strategy for obtaining seed cells for use in nerve tissue engineering.Electronic supplementary materialThe online version of this article (10.1186/s13287-018-0884-3) contains supplementary material, which is available to authorized users.

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Neurogenic differentiation capacity of subacromial bursal tissue—derived stem cells

Cited by 11 publications

References 26 publications

Characterization of bursa subacromialis-derived mesenchymal stem cells

Characterization of bursa subacromialis-derived mesenchymal stem cells

Editorial Commentary: Subacromial Bursa—Friend or Foe Within The Shoulder? An Old Debate With New Insights

Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function

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