Neurogenic differentiation of dental pulp stem cells to neuron-like cells in dopaminergic and motor neuronal inductive media

Chang, Chia-Chieh; Chang, Kai‐Chun; Tsai, Shiow-Chwen; Chang, Hao-Hueng; Lin, Chang‐Ching

doi:10.1016/j.jfma.2014.09.003

Cited by 85 publications

(81 citation statements)

References 37 publications

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“…Regarding the cell culture conditions, a variety of substrates, predominantly poly-l-lysine [36, 57, 140], poly-l-ornithine with/without lamin [147], gelatin [4, 47], and more rarely other substrates, have been used, while in most studies direct culture in culture-treated polystyrene [61, 125, 135, 137, 138, 141, 144] forms the commonest practice. However, the absence of comparative studies makes conclusions about the superiority of one substrate over the other impossible.…”

Section: Differentiation Potential and Paracrine Activity Of Dentamentioning

confidence: 99%

“…Regarding the neuroinductive culture media, most studies use either the Neurobasal A or conventional primarily Dulbecco's Modified Eagle's Medium (DMEM)/F12 media in their neural differentiation protocols. These are used in conjunction with various neural supplements (most commonly the B27 [36, 125, 142, 144, 148, 149], but also the N2 consisting of a mixture of insulin, transferrin, progesterone, selenium, and putrescine [137] and the insulin-transferrin-selenium (ITS) supplement [54] or their combinations [143]) in a serum-free approach. Alternatively, in other studies, the media are supplemented with conventional fetal calf (FCS) or Fetal Bovine Serum (FBS) at least for the first-stage preincubation phase [135].…”

Section: Differentiation Potential and Paracrine Activity Of Dentamentioning

confidence: 99%

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Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application

Bakopoulou

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2016

Stem Cells International

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Dental Mesenchymal Stem Cells (MSCs), including Dental Pulp Stem Cells (DPSCs), Stem Cells from Human Exfoliated Deciduous teeth (SHED), and Stem Cells From Apical Papilla (SCAP), have been extensively studied using highly sophisticated in vitro and in vivo systems, yielding substantially improved understanding of their intriguing biological properties. Their capacity to reconstitute various dental and nondental tissues and the inherent angiogenic, neurogenic, and immunomodulatory properties of their secretome have been a subject of meticulous and costly research by various groups over the past decade. Key milestone achievements have exemplified their clinical utility in Regenerative Dentistry, as surrogate therapeutic modules for conventional biomaterial-based approaches, offering regeneration of damaged oral tissues instead of simply “filling the gaps.” Thus, the essential next step to validate these immense advances is the implementation of well-designed clinical trials paving the way for exploiting these fascinating research achievements for patient well-being: the ultimate aim of this ground breaking technology. This review paper presents a concise overview of the major biological properties of the human dental MSCs, critical for the translational pathway “from bench to clinic.”

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Section: Differentiation Potential and Paracrine Activity Of Dentamentioning

confidence: 99%

Section: Differentiation Potential and Paracrine Activity Of Dentamentioning

confidence: 99%

Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application

Bakopoulou

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2016

Stem Cells International

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“…In 2000, a new group of adult mesenchymal stem cells, namely as dental stem cells were found to be the source of embryonic and mesenchymal cells with different biological characteristics [456]. These cells can be derived from human dental pulp and periodontal ligament.…”

Section: Introductionmentioning

confidence: 99%

Condition medium of cerebrospinal fluid and retinoic acid induces the transdifferentiation of human dental pulp stem cells into neuroglia and neural like cells

et al. 2017

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Cerebrospinal fluid (CSF) contains several molecules which are essential for neurogenesis. Human dental pulp stem cells (hDPSCs) are putatively neural crest cell-derived that can differentiate into neurons and glial cells under appropriate neurotrophic factors. The aim of this study was to induce differentiation of hDPSCs into neuroglial phenotypes using retinoic acid (RA) and CSF. The hDPSCs from an impacted third molar were isolated by mechanical and digestion and cultured. The cells have treated by 10−7 µM RA (RA group) for 8 days, 10% CSF (CSF group) for 8 days and RA with CSF for 8 days (RA/CSF group). Nestin, microtubule-associated protein 2 (MAP2), and glial fibrillary acidic protein immunostaining were used to examine the differentiated cells. Axonal outgrowth was detected using Bielschowsky's silver impregnation method and Nissl bodies were stained in differentiated cells by Cresyl violet. The morphology of differentiated cells in treated groups was significantly changed after 3–5 days. The results of immunocytochemistry showed the presence of neuroprogenitor marker nestin was seen in all groups. However, the high percentage of nestin positive cells and MAP2, as mature neural markers, were observed at the pre-induction and induction stage, respectively. Nissl bodies were detected as dark-blue particles in the cytoplasm of treated cells. Our findings showed the RA as pre-inducer and CSF as inducer for using in vitro differentiation of neuron-like cells and neuroglial cells from hDPSCs.

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“…Previous research had shown that the dental pulp tissue of adult teeth contained neural-crest derived DPSCs, which expressed neural stem cell markers such as Nestin and GFAP 4,11 . Moreover, these were fully viable after one month post-graft and capable of in vitro differentiating into mesenchymal cell lineages and generating a dentin pulp-like tissue complex, after in vivo subcutaneous transplantation 3 .…”

Section: Neurocult Proliferation Mediamentioning

confidence: 99%

Human Dental Pulp Stem Cells Grown in Neurogenic Media Differentiate into Endothelial Cells and Promote Neovasculogenesis in the Mouse Brain

Luzuriaga

Pastor-Alonso

Encinas

et al. 2018

Preprint

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Financial support: «Ramón y Cajal » program RYC-2013-13450 (JRP) and RYC 2012-11137 (JME); Spanish Ministery of Economy and Competitivity SAF2015-70866-R, University of the Basque Country (GIU16/66, UFI 11/44), and Basque Government (GV/EJ; IT831-13).Running title: Human DPSCs integrate into brain endothelial vasculature. Main text (not including Abstract, Methods, References and figure legends): 4005 words Total number of figures: 6References: 65 SUMMARYDental Pulp Stem Cells (DPSCs) have a demonstrated capacity to acquire neuronal-like phenotypes, suggesting their use in brain cell therapies. In the present work, we wanted to address the phenotypic fate of adult DPSCs cultured in Neurocult media (Stem Cell Technologies), a cell culture medium without serum routinely used for the expansion of adult neural stem cells (NSCs). Our results showed for the first time, that non-genetically modified adult DPSCs cultured with Neurocult generated neurosphere-like dentospheres expressing the NSC markers Nestin and GFAP, but also the vascular endothelial cell marker CD31. One month post-intracranial graft into athymic nude mice, human CD31+ or Nestin+ DPSCderived cells were found tightly associated with brain blood vessels increasing their laminin staining. These results suggest that DPSCs integrated and contributed to an increased generation of neovasculature within brain tissue and that Neurocult medium constituted a fast and efficient way to obtain endothelial cells from human DPSCs.

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Neurogenic differentiation of dental pulp stem cells to neuron-like cells in dopaminergic and motor neuronal inductive media

Cited by 85 publications

References 37 publications

Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application

Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application

Condition medium of cerebrospinal fluid and retinoic acid induces the transdifferentiation of human dental pulp stem cells into neuroglia and neural like cells

Human Dental Pulp Stem Cells Grown in Neurogenic Media Differentiate into Endothelial Cells and Promote Neovasculogenesis in the Mouse Brain

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