Neuroimmunomodulation by allogeneic seminal vesicle fluid in CNS is sex-independent

Abstract

This study suggests that seminal vesicle fluid from Wistar rats has anti-inflammatory effect on Lewis rats in a sex-independent manner. In addition, seminal vesicle fluid from Lewis rats had not beneficial effect on EAE in male Lewis rats. This is consistent with Tregs increase in allogeneic mating. More research is required to find out the immunologic aspect of allogeneic versus syngeneic administration of seminal vesicle fluid.

New Paradigm in Cell Therapy Using Sperm Head to Restore Brain Function and Structure in Animal Model of Alzheimer’s Disease: Support for Boosting Constructive Inflammation vs. Anti-Inflammatory Approach

“…To prepare sperm head, a pool of sperm was prepared from five Wistar rats and sperm head was prepared following three steps of centrifuging and using ficoll and SVF was prepared from mature male rats as described before [ 23 , 24 ]. Sperm and SVF were prepared from Wistar rats to make sure that this method overcomes genetic differences which is an important obstacle in stem cell therapy.…”

“…The sequences for primers were as forward 5′-ATCTGGCACCACACCTTC-3′ and reverse 5′-AGCCAGGTCCAGACGCA-3′ for β-actin , forward 5′-AAGTTCAACGGCACAGTCAAGG-3′ and reverse 5′-CATACTCAGCACCAGCATCACC-3′ for GAPDH , forward 5′- AGGTATGGATGGGTTGGAGA-3′ and reverse 5′- GGGGTTTGAGTGTTGAATGTGG-3′ for LYVE-1 , forward 5′-GGTCTTCAGGAGAAGGGAATG-3′ and reverse 5′- GGTATTCCAGTGTGTGAAGTG-3′ for Prox1 , forward 5′-GTTGGTCTGGGTTTTGGGG-3′ and reverse 5′-CAATGGGAGGCTGTGTTGGT-3′ for PDPN , forward 5′-TGGGCAGGAGGTGTTGTGGGA-3′ and reverse 5′-GAGGAAGGGATTGGAAAGGA-3′ for VEGF-R3 , and forward 5′-GATGTGGGGAAGGAGTTTGGAG-3′ and reverse 5′-CTGATTGTGACTGGTTTGGGG-3′ for VEGF-C . The primer's efficiency and specificity, fidelity of real-time PCR, and melting curve analysis were controlled as described before [ 23 , 24 ]. Thermocycler conditions commenced with an initial step at 95°C for 15 min.…”

“…As an example, autoimmune attack in multiple sclerosis is ameliorated during pregnancy [ 21 , 22 ]. Studies on reproductive immunology have shown that seminal vesicle fluid (SVF) has anti-inflammatory property and increases Tregs [ 22 – 24 ]. In contrast, sperm/sperm head promotes a constructive inflammatory response by recruitment and programming of neutrophils leading to elimination of debridement and tissue remodeling and reconstitution in the uterus during mating suitable for the fetus [ 25 – 27 ].…”