Neuroimmunomodulation: Neuroendocrine Regulation of Immunity: The Effects of Noradrenaline inXenopus Laevis, the South African Clawed Toad

Clothier, Richard H.; Ruben, Laurens N.; Johnson, Rachel O.; Parker, Katherine L.; Sovak, Mika; Greenhalgh, L; Ooi, Eng Eong; Balls, Michael

doi:10.3109/00207459108999766

Cited by 9 publications

(11 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ß 2 -Adrenoceptor ligation of lectin-stimulated Xenopus splenocytes also reduced mitogenesis [9]. This inhibition was blocked with appropriate receptor-specific antagonists, indicating that receptor ligation was responsible for the physiological activity.…”

Section: Introductionmentioning

confidence: 88%

“…Adrenoceptor dependence of the effects of isoproterenol were tested with one of the three apoptogens used, the ionophore A23187, by blocking with a tenfold excess of the ß-adrenoceptor antagonist, propanolol, added 5 min before exposure to the agonist. Two more complete explorations of clonidine and isoproterenol on immune reactivity, using adult Xenopus splenocytes, have been reported previously [2,9].…”

Section: Cell Preparation and Culturementioning

confidence: 99%

“…The precise concentrations selected were 10 -10 M for clonidine, 10 -6 M for isoproterenol, 50 ng/ml for A23187, 10 -7 M for DEX and 150 ng/ml for PMA. A concentration of 10 -6 M had been used in prior functional studies using these adrenergic reagents with Xenopus splenocytes [2,9].…”

Section: Cell Preparation and Culturementioning

confidence: 99%

“…As adrenergic secretions are so effective in modulating immune function in Xenopus [9], the potential for regulation of apoptosis by stress-related molecules is an important consideration. The modulating effects of · 2 -and ß-adrenergic agonists alone or in combination with the apoptogens, A23187, a calcium ionophore, dexamethasone (DEX), a high-affinity-binding synthetic glucocorticoid, or phorbol 12-myristate-13-acetate (PMA), a phorbol diester, are examined in vitro, using splenocytes from postmetamorphic Xenopus.…”

Section: Introductionmentioning

confidence: 99%

“…the secretion of TNF-· [7], and the regulation of antibody production in mammals [8] and Xenopus [9].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Adrenergic Modulation of Apoptosis in Splenocytes of Xenopus laevis in vitro

Haberfeld

Johnson²,

Ruben³

et al. 1999

Neuroimmunomodulation

Self Cite

View full text Add to dashboard Cite

Impaired and healthy cells undergo suicide using an intrinsic genetic program. Exposure to stress-related α₂- or β-adrenergics for 4 or 20 h in vitro had no effect on apoptosis in splenocytes of adult Xenopus laevis, while a 4-hour coincubation of clonidine, an α₂-agonist, with a calcium ionophore (A23187) or a phorbol diester (PMA), enhanced apoptosis induced by each apoptogen alone. Clonidine did not affect apoptosis stimulated with dexamethasone (DEX), however. Comparable in vitro exposures to isoproterenol, a β-agonist, reduced apoptotic levels stimulated by all three apoptogens alone. Following 20 h coexposure, clonidine no longer affected A23187-induced apoptosis, but reduced PMA-induced apoptosis, while isoproterenol enhanced apoptosis stimulated with both. Neither agonist modulated apoptosis induced by 20 h of exposure to DEX. Thus, adrenergic agonists modulated apoptosis in cells coexposed to A23187 and PMA, in a time-dependent and adrenoceptor class-dependent fashion. These stress-induced products can affect concurrent apoptosis reversibly over time in vitro, and thus possibly in vivo.

show abstract

Section: Introductionmentioning

confidence: 88%

Section: Cell Preparation and Culturementioning

confidence: 99%

Section: Cell Preparation and Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…the secretion of TNF-· [7], and the regulation of antibody production in mammals [8] and Xenopus [9].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Adrenergic Modulation of Apoptosis in Splenocytes of Xenopus laevis in vitro

Haberfeld

Johnson²,

Ruben³

et al. 1999

Neuroimmunomodulation

Self Cite

View full text Add to dashboard Cite

show abstract

Noradrenergic and peptidergic innervation of lymphoid organs in the beluga, Delphinapterus leucas: An anatomical link between the nervous and immune systems

Felten¹,

Olschowka²,

Felten³

1994

Journal of Morphology

View full text Add to dashboard Cite

The presence of peptidergic and noradrenergic sympathetic nerve fibers in specific compartments of both primary and secondary lymphoid organs of the rodent is well established. These nerve fibers directly contact lymphocytes and macrophages, as well as vascular and trabecular smooth muscle. We investigated the noradrenergic and neuropeptide-Y innervation of lymphoid organs in the cetacean, Delphinapterus leucas (beluga whale). The spleen, thymus, tonsil, gut-associated lymphoid tissue, and assorted lymph nodes were collected from five belugas, obtained during sanctioned hunts, and processed for catecholamine fluorescence histochemistry and for tyrosine hydroxylase and neuropeptide-Y immunocytochemistry. Innervation studies revealed fluorescent nerve fibers, tyrosine hydroxylase, and neuropeptide-Y positive nerve fibers in parenchymal lymphoid compartments, where they were closely associated with cells of the immune system, and in vascular and trabecular compartments. In lymphoid zones, tyrosine hydroxylase and neuropeptide-Y positive nerve fibers were observed in the periarteriolar lymphatic sheath and marginal zone of the spleen; in the outermost portion of the cortex, the corticomedullary zone, and medulla of the lymph nodes; in the parafollicular zones, and diffuse lymphocyte layer below the epithelium of the tonsil; in the outermost portion of some thymic lobules; and in the lamina propria of the gut. These findings are similar to those described for other mammals and substantiate an anatomical link between the nervous and immune systems in the beluga, whereby central nervous system activity may influence autonomic outflow to lymphoid organs and effect immunologic reactivity.

show abstract

Sympathetic innervation of the amphibian spleen: Developmental studies in Xenopus laevis

Kinney¹,

Felten²,

Cohen

1996

Developmental & Comparative Immunology

View full text Add to dashboard Cite

Neuroimmunomodulation: Neuroendocrine Regulation of Immunity: The Effects of Noradrenaline inXenopus Laevis, the South African Clawed Toad

Cited by 9 publications

References 26 publications

Adrenergic Modulation of Apoptosis in Splenocytes of <i>Xenopus laevis</i> in vitro

Adrenergic Modulation of Apoptosis in Splenocytes of <i>Xenopus laevis</i> in vitro

Noradrenergic and peptidergic innervation of lymphoid organs in the beluga, Delphinapterus leucas: An anatomical link between the nervous and immune systems

Sympathetic innervation of the amphibian spleen: Developmental studies in Xenopus laevis

Contact Info

Product

Resources

About