Neurokinin-1 Receptors Located in Human Retinoblastoma Cell Lines: Antitumor Action of Its Antagonist, L-732,138

Muñoz, Miguel; Rosso, Marisa; Coveñas, Rafael; Montero, Ignacio; González‐Moles, Miguel Ángel; Robles, María José

doi:10.1167/iovs.05-1591

Cited by 56 publications

(55 citation statements)

References 32 publications

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“…Cell nuclei were lightly counterstained with hematoxylin. Finally, as previously published [21] , in order to determine the specificity of the immunostaining, primary retinoblastoma was used as positive control. As negative control, the primary antibody was omitted, being replaced by nonimmune serum.…”

Section: Immunohistochemistry For Nk-1 Receptors In Human Primary Adementioning

confidence: 99%

The NK-1 Receptor Is Expressed in Human Primary Gastric and Colon Adenocarcinomas and Is Involved in the Antitumor Action of L-733,060 and the Mitogenic Action of Substance P on Human Gastrointestinal Cancer Cell Lines

Rosso¹,

Robles‐Frías²,

Coveñas³

et al. 2008

Tumor Biol

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Background/Aims: It has been demonstrated that substance P (SP) and neurokinin-1 (NK-1) receptor antagonist L-733,060 induces cell proliferation and inhibition, respectively, in several human cancer cell lines. At present, it is unknown whether such actions are exerted on human gastric and colon adenocarcinomas. We carried out an in vitro study of the growth-inhibitory capacity of L-733,060 against human gastric and colon adenocarcinomas. Methods: A coulter counter was used to determine viable cell numbers followed by application of the tetrazolium compound MTS. Immunoblot analysis was used to determine the NK-1 receptors and the DAPI method was applied to demonstrate apoptosis. Immunohistochemistry was used to demonstrate NK-1 receptors in primary human gastric and colon adenocarcinomas. Results: We observed the presence of several NK-1 receptor isoforms in human gastric and colon adenocarcinomas. Nanomolar concentrations of SP increased the growth of both cell lines and micromolar concentrations of L-733,060 inhibited the growth of such cell lines, with and without previous administration of SP. L-733,060 inhibited the growth of the 23132/87 and SW-403 cell lines in a dose-dependent manner. After administration of L-733,060, apoptosis was observed in both cell lines. In both human primary gastric and colon adenocarcinomas, a high density of NK-1 receptors was observed. Immunoreactivity, showing a diffuse cytoplasmic staining, was observed in the epithelial cells of normal and tumor glands and in numerous stromal elements. Conclusions: We demonstrated that NK-1 receptors were expressed in 23132/37 and SW-403 cell lines and in human primary gastric and colon adenocarcinomas, that SP is a mitogen and that the antitumor action of L-733,060 on both human cell lines occurs through the NK-1 receptor. Data also indicate that the cell death observed is produced by apoptosis. These data suggest that the NK-1 receptor is a new and promising target in the treatment of human gastrointestinal adenocarcinomas.

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Section: Immunohistochemistry For Nk-1 Receptors In Human Primary Adementioning

confidence: 99%

The NK-1 Receptor Is Expressed in Human Primary Gastric and Colon Adenocarcinomas and Is Involved in the Antitumor Action of L-733,060 and the Mitogenic Action of Substance P on Human Gastrointestinal Cancer Cell Lines

Rosso¹,

Robles‐Frías²,

Coveñas³

et al. 2008

Tumor Biol

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“…(a) by inhibiting tumor cell proliferation in several tumor cell lines, including breast cancer cells [10,13,14]. NK-1 receptor antagonists induce cellular death by apoptosis [10,14]; (b) by inhibiting tumor neoangiogenesis [15]; and (c) by inhibiting the migration of tumoral cells (e.g., breast carcinoma cells) [9,16].…”

Section: Nk-1 Receptor Antagonists Exert An Antitumor Actionmentioning

confidence: 99%

“…NK-1 receptor antagonists induce cellular death by apoptosis [10,14]; (b) by inhibiting tumor neoangiogenesis [15]; and (c) by inhibiting the migration of tumoral cells (e.g., breast carcinoma cells) [9,16]. NK-1 receptor antagonists also block the mitogenic action exerted by SP on tumor cells.…”

Section: Nk-1 Receptor Antagonists Exert An Antitumor Actionmentioning

confidence: 99%

Paravertebral anesthesia: how substance P and the NK-1 receptor could be involved in regional block and breast cancer recurrence

Muñoz

Rosso

Casinello

et al. 2010

Breast Cancer Res Treat

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“…Indeed, TACR1 inhibitors were discussed as possible anticancer agents. 75 Second, it has been argued that death signaling is commonly impaired in malignancies, so protection of normal cells through targeting of these same pathways may not affect the efficacy of treatment, at least, for some cancers. 76 Third, an acute nephroprotective intervention may be too short to sustain tumor growth.…”

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confidence: 99%

Erratum: An RNA interference screen identifies new avenues for nephroprotection

et al. 2016

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Acute kidney injury is a major public health problem, which is commonly caused by renal ischemia and is associated with a high risk of mortality and long-term disability. Efforts to develop a treatment for this condition have met with very limited success. We used an RNA interference screen to identify genes (BCL2L14, BLOC1S2, C2ORF42, CPT1A, FBP1, GCNT3, RHOB, SCIN, TACR1, and TNFAIP6) whose suppression improves survival of kidney epithelial cells in in vitro models of oxygen and glucose deprivation. Some of the genes also modulate the toxicity of cisplatin, an anticancer agent whose use is currently limited by nephrotoxicity. Furthermore, pharmacological inhibition of TACR1 product NK1R was protective in a model of mouse renal ischemia, attesting to the in vivo relevance of our findings. These data shed new light on the mechanisms of stress response in mammalian cells, and open new avenues to reduce the morbidity and mortality associated with renal injury.

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Neurokinin-1 Receptors Located in Human Retinoblastoma Cell Lines: Antitumor Action of Its Antagonist, L-732,138

Cited by 56 publications

References 32 publications

The NK-1 Receptor Is Expressed in Human Primary Gastric and Colon Adenocarcinomas and Is Involved in the Antitumor Action of L-733,060 and the Mitogenic Action of Substance P on Human Gastrointestinal Cancer Cell Lines

The NK-1 Receptor Is Expressed in Human Primary Gastric and Colon Adenocarcinomas and Is Involved in the Antitumor Action of L-733,060 and the Mitogenic Action of Substance P on Human Gastrointestinal Cancer Cell Lines

Paravertebral anesthesia: how substance P and the NK-1 receptor could be involved in regional block and breast cancer recurrence

Erratum: An RNA interference screen identifies new avenues for nephroprotection

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