Neuromedin B regulates steroidogenesis, cell viability and apoptosis in rabbit Leydig cells

Ma, Zhiyu; Guo, Junpei; Zhang, Ying; Zhang, Youwen; Zhang, Miao; Zong, Rongling; Chen, Fenglei; Zhang, Jinlong

doi:10.1016/j.ygcen.2019.113371

Cited by 2 publications

(1 citation statement)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cell viability was assessed using the Cell Counting Kit-8 (Beyotime Co. Ltd., Shanghai, China) according to the manufacturer's instructions and as previously validated (Ma et al 2020). Briefly, bovine granulosa and theca-derived cells (in a 3:1 ratio) were cultured in 96-well plates (2 × 10 3 cells/well) for 24h, and then treated with 0 or 10 ng.mL -1 TGFB1 in the presence or absence of pre-treatment with SB431542 (10 μΜ) for 48h.…”

Section: Cell Viability Assaymentioning

confidence: 99%

Transforming growth factor-β1 disrupts angiogenesis during the follicular–luteal transition through the Smad–serpin family E member 1 (SERPINE1)/serpin family B member 5 (SERPINB5) signalling pathway in the cow

Yan

et al. 2021

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

Intense angiogenesis is critical for the development of the corpus luteum and is tightly regulated by numerous factors. However, the exact role transforming growth factor-β1 (TGFB1) plays during this follicular–luteal transition remains unclear. This study hypothesised that TGFB1, acting through TGFB receptor 1 (TGFBR1) and Smad2/3 signalling, would suppress angiogenesis during the follicular–luteal transition. Using a serum-free luteinising follicular angiogenesis culture system, TGFB1 (1 and 10ngmL–1) markedly disrupted the formation of capillary-like structures, reducing the endothelial cell network area and the number of branch points (P<0.001 compared with control). Furthermore, TGFB1 activated canonical Smad signalling and inhibited endothelial nitric oxide synthase (NOS3) mRNA expression, but upregulated latent TGFB-binding protein and TGFBR1, serpin family E member 1 (SERPINE1) and serpin family B member 5 (SERPINB5) mRNA expression. SB431542, a TGFBR1 inhibitor, reversed the TGFB1-induced upregulation of SERPINE1 and SERPINB5. In addition, TGFB1 reduced progesterone synthesis by decreasing the expression of steroidogenic acute regulatory protein (STAR), cytochrome P450 family 11 subfamily A member 1 (CYP11A1) and 3β-hydroxysteroid dehydrogenase (HSD3B1) expression. These results show that TGFB1 regulates NOS3, SERPINE1 and SERPINB5 expression via TGFBR1 and Smad2/3 signalling and this could be the mechanism by which TGFB1 suppresses endothelial networks. Thereby, TGFB1 may provide critical homeostatic control of angiogenesis during the follicular–luteal transition. The findings of this study reveal the molecular mechanisms underlying the actions of TGFB1 in early luteinisation, which may lead to novel therapeutic strategies to reverse luteal inadequacy.

show abstract

Section: Cell Viability Assaymentioning

confidence: 99%

Transforming growth factor-β1 disrupts angiogenesis during the follicular–luteal transition through the Smad–serpin family E member 1 (SERPINE1)/serpin family B member 5 (SERPINB5) signalling pathway in the cow

Yan

et al. 2021

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

show abstract

High-Precision Detection of Cellular Drug Response Based on SERS Spectrum and Multivariate Statistical Analysis

Wang

et al. 2023

Biosensors

View full text Add to dashboard Cite

The rapid development of personalized medicine places high demands on the control of drug dose and cellular drug response to provide patients with better curative effects and low side effects. To solve the problem of low detection accuracies of the cell-counting kit-8 (CCK8) method, a detection method based on surface-enhanced Raman spectroscopy (SERS) of cell-secreted proteins was adopted to evaluate the concentration of the anticancer drug cisplatin and the cellular drug response of nasopharyngeal carcinoma. CNE1 and NP69 cell lines were used to evaluate cisplatin response. The results showed that the combination of the SERS spectrum with principal component analysis–linear discriminant analysis could detect the difference in the response of cisplatin with a concentration difference of 1 μg/mL, which considerably exceeded that of CCK8. In addition, the SERS spectral peak intensity of the cell-secreted proteins strongly correlated with the cisplatin concentration. Furthermore, the mass spectrum of the secreted proteins of the nasopharyngeal carcinoma cells was analyzed to verify the results obtained using the SERS spectrum. The results demonstrated that SERS of secreted proteins has great potential for high-precision detection of chemotherapeutic drug response.

show abstract

Neuromedin B regulates steroidogenesis, cell viability and apoptosis in rabbit Leydig cells

Cited by 2 publications

References 22 publications

Transforming growth factor-β1 disrupts angiogenesis during the follicular–luteal transition through the Smad–serpin family E member 1 (SERPINE1)/serpin family B member 5 (SERPINB5) signalling pathway in the cow

Transforming growth factor-β1 disrupts angiogenesis during the follicular–luteal transition through the Smad–serpin family E member 1 (SERPINE1)/serpin family B member 5 (SERPINB5) signalling pathway in the cow

High-Precision Detection of Cellular Drug Response Based on SERS Spectrum and Multivariate Statistical Analysis

Contact Info

Product

Resources

About