Neuromedin-N Is not Released with Neurotensin from Rat Ileum

Cuber, Jean‐Claude; Herrmann, Christine; Kitabgi, Patrick; Bosshard, A.; Bernard, Christine; Nadai, François de; Chayvialle, Jean‐Alain

doi:10.1210/endo-126-3-1584

Cited by 30 publications

(27 citation statements)

References 28 publications

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“…The surgical steps and functional viability of the isolated vascularly perfused rat ileum have been previously reported in detail (13). Male Wistar rats (250 -300 g) were anesthetized with sodium pentobarbitone (50 mg/kg, ip), and the abdomen was opened with a midline incision.…”

Section: Surgical Preparationmentioning

confidence: 99%

“…NT-like immunoreactivity (NT-LI) in the portal effluent was measured with an antiserum specific for intact NT, as previously described (13,14). Briefly, antiserum 29G was obtained in a rabbit after repeated injection of NT conjugated to BSA and was used at a final dilution of 1:200,000.…”

Section: Riasmentioning

confidence: 99%

“…The sensitivity and ID 50 were 0.6 and 3.0 fmol/tube, respectively. HPLC analysis of portal supernatants followed by RIA with antiserum 29G revealed a single peak coeluting with NT-(1-13) (13).…”

Section: Riasmentioning

confidence: 99%

“…Overall, little is known about the comparative responsiveness of these two cell types upon stimulation with the individual components of the intestinal chyme. This was the purpose of the present study conducted with the isolated vascularly perfused rat ileum preparation (13). This model provides a unique opportunity to study the secretion of NT, PYY, and GLP-1 in response to well defined luminal stimuli in a manner that eliminates the influences potentially encountered in vivo.…”

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confidence: 99%

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Peptide YY, Glucagon-Like Peptide-1, and Neurotensin Responses to Luminal Factors in the Isolated Vascularly Perfused Rat Ileum

et al. 1998

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Exposure of the ileum to nutrients markedly inhibits several upper gastrointestinal functions. Hormonal peptides of the ileal wall, i.e. peptide YY (PYY), glucagon-like peptide-1 (GLP-1), and neurotensin (NT), are thought to play a role in this negative feedback mechanism. The present study was conducted to comparatively assess the secretion of PYY, GLP-1, and NT upon luminal infusion of a variety of individual luminal factors in the isolated vascularly perfused rat ileum preparation. PYY, GLP-1, and NT were measured in the portal effluent with specific RIAs. Glucose (250 mM) induced a pronounced release of the three peptides, whereas a physiological concentration of 5 mM did not induce peptide secretion. Peptone (5%, wt/vol) evoked a sustained release of PYY, GLP-1, and NT. Only NT secretion was increased upon luminal administration of 100 mM sodium oleate.Short chain fatty acids (20 mM) evoked an early and transient release of the three peptides. In contrast, taurocholate (20 mM) induced a sustained release of PYY, GLP-1, and NT, but the threshold concentration for peptide release was lower for NT than for PYY or GLP-1. Cellulose or pectin (0.5%, wt/vol) did not modify peptide secretion. In conclusion, glucose and peptone are potent stimulants of PYY, GLP-1, and NT release. Only NT is released upon oleic acid stimulation. Finally, taurocholate is a potent stimulant of the release of the three peptides. Overall, PYY, GLP-1, and NT may participate cooperatively in the ileal brake. As relatively high concentrations of the various stimulants were required to elicit peptide release, it seems likely that this mechanism operates in cases of maldigestion or malabsorption. (Endocrinology 139: 3780 -3786, 1998) U NABSORBED nutrients reaching the ileum influence the functions of the upper gut to increase the efficiency of digestion and absorption. For example, unabsorbed fat or protein in the ileum delays the passage of material through the small intestine. As a delay in small bowel transit increases the contact time between luminal contents and absorptive epithelium, this mechanism may serve to increase the absorption of a meal. This negative feedback mechanism referred to as the ileal brake presumably involves hormonal intermediates. As the ileal mucosa contains several distinct populations of endocrine cells, many studies focused on the effects of the products of these cells on gastrointestinal functions. Neurotensin (NT), the major product of the so-called N cells, and peptide YY (PYY) and glucagon-like peptide 1 (GLP-1), which are cosynthesized in the so-called L cells, have been implicated in these feedback mechanisms.Circulating levels of NT, PYY, and GLP-1 rapidly increase in response to oral ingestion of a mixed meal, thus suggesting that their release is triggered in part by hormonal and/or neural signals originating from the upper small intestine. As NT, PYY, and GLP-1 are contained in open-type cells, nutrients making contact with the ileal mucosa are also capable of eliciting peptide secretion. In...

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Section: Surgical Preparationmentioning

confidence: 99%

Section: Riasmentioning

confidence: 99%

Section: Riasmentioning

confidence: 99%

mentioning

confidence: 99%

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Peptide YY, Glucagon-Like Peptide-1, and Neurotensin Responses to Luminal Factors in the Isolated Vascularly Perfused Rat Ileum

et al. 1998

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“…The operative procedure was similar to that previously described by Cuber et al 20,21 for the isolated, vascu- …”

Section: Animal Preparationmentioning

confidence: 99%

The Effect of Urocortin 1 on Motility in Isolated, Vascularly Perfused Rat Colon

You

Lee

Kim

et al. 2015

Korean J Gastroenterol

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Background/Aims: Urocortin 1, a corticotropin-releasing factor related peptide, increases colonic motility under stressful conditions. We investigated the effect of urocortin 1 on colonic motility using an experimental model with isolated rat colon in which the blood flow and intestinal nerves were preserved. Furthermore, we assessed whether this effect was mediated by adrenergic or cholinergic nerves. Methods: Colonic motility was measured in the proximal and distal parts of resected rat colon. The colon resected from the peritoneum was stabilized, and then urocortin 1 (13.8, 138, 277, and 1,388 pM) was administered via a blood vessel. Motility index was measured in the last 5 min of the 15 min administration of urocortin 1 and expressed as percentage change from baseline. Subsequently, the change in motility was measured by perfusing urocortin 1 in colons pretreated with phentolamine, propranolol, hexamethonium, atropine, or tetrodotoxin. Results: At concentrations of 13.8, 138, 277, and 1,388 pM, urocortin 1 increased the motility of proximal colon (20.4±7.2%, 48.4±20.9%, 67.0±25.8%, and 64.2±20.9%, respectively) and the motility of distal colon (3.3±3.3%, 7.8±7.8%, 71.1±28.6%, and 87.4±32.5%, respectively). The motility induced by urocortin 1 was significantly decreased by atropine to 2.4±2.4% in proximal colon and 3.4±3.4% in distal colon (p＜0.05). However, tetrodotoxin, propranolol, phentolamine, and hexamethonium did not inhibit motility. Conclusions: Urocortin 1 increased colonic motility and it is considered that this effect was directly mediated by local muscarinic cholinergic receptors. (Korean J Gastroenterol 2015;65:283-290)

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Distribution of neurotensin‐containing neurons in the central nervous system of the dog

Atoji

Watanabe

Yamamoto

et al. 1995

J of Comparative Neurology

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The distribution of neurotensin-containing cell bodies and fibers was examined in the central nervous system of the dog using light microscopic immunohistochemistry. A very large population of neurotensin-containing cell bodies was observed in the septal nuclei, nucleus accumbens septi, preoptic areas, bed nucleus of the stria terminalis, olfactory tubercle, entorhinal cortex, ventral subiculum, anterodorsal thalamic nucleus, anteroventral thalamic nucleus, nucleus reuniens, lateral habenular nucleus, parabrachial nucleus, and nucleus of the solitary tract. Extremely dense networks of neurotensin-containing fibers were found in the globus pallidus, hypothalamus, infundibular stalk, ventral tegmental area, periaqueductal gray, interpeduncular nucleus, and spinal nucleus of the trigeminal nerve and substantia gelatinosa. However, the cerebral neocortex and cerebellum were negative for neurotensin in the present study. When the present findings are compared with those in other animals, it is clear that the major species-specific differences in distribution involve three immunonegative regions and four immunopositive regions in the dog: The former are the cerebral neocortex, mammillary body, and hippocampus; the latter are the cell bodies in the pyramidal layer of the olfactory tubercle, the superficial and middle layers of the entorhinal cortex and ventral subiculum, and the nerve fibers in the interpeduncular nucleus. The present study indicates a rather extensive network of neurotensin neurons in the central nervous system of the dog.

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Neuromedin-N Is not Released with Neurotensin from Rat Ileum

Cited by 30 publications

References 28 publications

Peptide YY, Glucagon-Like Peptide-1, and Neurotensin Responses to Luminal Factors in the Isolated Vascularly Perfused Rat Ileum

Peptide YY, Glucagon-Like Peptide-1, and Neurotensin Responses to Luminal Factors in the Isolated Vascularly Perfused Rat Ileum

The Effect of Urocortin 1 on Motility in Isolated, Vascularly Perfused Rat Colon

Distribution of neurotensin‐containing neurons in the central nervous system of the dog

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