Neuron-glia interactions in the release of oxytocin and vasopressin from the rat neural lobe: The role of opioids, other neuropeptides and their receptors

Boersma, C.J.C.; Leeuwen, Fred W. van

doi:10.1016/0306-4522(94)90339-5

Cited by 58 publications

(39 citation statements)

References 137 publications

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“…1A, 2A, B), consistent with a putative association with VP nerve terminals that are enriched in this area, relative to OT terminals that are more commonly found in the narrow outer regions of the NL [13, 14]. The GALP mRNA hybridization signal was quite strong with silver grains overlying individual cells within the NL (fig.…”

Section: Resultsmentioning

confidence: 58%

“…Since many previous studies have shown that osmotic stimulation alters VP and OT content, release and mRNA levels in the posterior lobe of the pituitary [7, 8, 9, 19, 20], and as such stimuli also produce a range of morphological and other biochemical changes within the NL [14, 19, 20], we examined whether dehydration or salt loading would modify GALP gene expression in the pituitary (fig. 1C–F, 2C–F; table 1).…”

Section: Resultsmentioning

confidence: 99%

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Galanin-Like Peptide mRNA in Neural Lobe of Rat Pituitary

2001

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Galanin-like peptide (GALP) was recently identified in the porcine hypothalamus, pituitary gland and gut, and has reported selectivity for the GalR2, c.f. the GalR1 receptor. GALP cDNAs have been cloned from pig, rat and human, and GALP mRNA expression is restricted to the arcuate nucleus in normal rat brain. This study examined the regional and cellular distribution of GALP mRNA in the rat pituitary gland, and subsequently determined the effect of osmotic stimulation on GALP transcript levels. GALP mRNA was not detected in the anterior or intermediate lobes, but moderate levels of GALP mRNA were present in the neural (posterior) lobe, in presumed pituicytes, of normal male and female rats. Osmotic stimulation by dehydration or salt loading produced a time-dependent increase in GALP mRNA levels in the neural lobe. Thus, dehydration for 4 days increased GALP mRNA 40-fold, while salt loading for 4, 7 or 10 days increased GALP levels 14-, 21- and 25-fold, respectively (p ≤ 0.001). Levels of vasopressin (VP) mRNA in the neural lobe were also increased by these treatments, consistent with previous reports. Galanin (GAL) and GalR2 receptor mRNAs were not detected in the neural lobe, under normal or osmotic stimulation conditions. In addition, GALP mRNA levels in the arcuate nucleus were not altered in dehydrated or salt-loaded rats; and GALP mRNA was not detected in magnocellular neurons of the supraoptic or paraventricular nucleus, despite the characteristic up-regulation of VP and GAL mRNA in these cells. In view of the established anatomy and function of VP/oxytocin neurons in the hypothalamo-neurohypophysial system and the role played by pituicytes in their regulation, the likely synthesis/release of GALP by these specialized astrocytes strongly suggests a role for this novel peptide in regulation of pituicyte morphology/function and/or neurohormone release.

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Section: Resultsmentioning

confidence: 58%

Section: Resultsmentioning

confidence: 99%

Galanin-Like Peptide mRNA in Neural Lobe of Rat Pituitary

2001

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“…The secretion of these neuropeptides is controlled by various regulators and may be The pituicytes were earlier presumed to play a role only in the storage of AVP and OXT [10]. However, we have shown, that the pituicytes are able to produce and secrete both AVP and OXT [13] and can be used as a model to investigate the secretion of AVP and OXT.…”

Section: Discussionmentioning

confidence: 75%

“…Besides their glial functions [9], the neurohypophyseal pituicytes may have roles in AVP and OXT expression. AVP and OXT were earlier dogmatic considered to be only stored and not produced in the NH [10], but our group [subsection 1.3] and others [11,12] have demonstrated that the NH is also the site of AVP and OXT expression. We have reported that radioimmunoassay (RIA) and spectrometry reveal that the secretion of these hormones in a NH model in vitro was elevated during 2 weeks [13].…”

Section: Introductionmentioning

confidence: 85%

Study of neurogenic involvement in arginine-vasopressin and oxytocin release under basal and environmentally stimulated conditions

Nagyéri¹

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“…Általánosan elfogadott tény, hogy a NH csak mint a VP raktározás színtere játszik szerepet, itt kerül a vérkeringésbe (22). Témavezetőm, Dr. László A. Ferenc és munkatársai tömegspektroszkópiás módszerrel azonosítottak izolált NH sejtkultúra felülúszó médiumában megjelenő VP-t (23) és leírták, hogy a pituicyták VP termelésre és kiválasztásra képesek (24).…”

Section: A Vazopresszin (Vp) Kiválasztás Centrális Regulációjaunclassified

Orexin jelentősége a vízanyagcsere, valamint a vazopresszin kiválasztás szabályozásában

Kis¹

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Neuron-glia interactions in the release of oxytocin and vasopressin from the rat neural lobe: The role of opioids, other neuropeptides and their receptors

Cited by 58 publications

References 137 publications

Galanin-Like Peptide mRNA in Neural Lobe of Rat Pituitary

Galanin-Like Peptide mRNA in Neural Lobe of Rat Pituitary

Study of neurogenic involvement in arginine-vasopressin and oxytocin release under basal and environmentally stimulated conditions

Orexin jelentősége a vízanyagcsere, valamint a vazopresszin kiválasztás szabályozásában

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